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. 1988 Nov;62(11):4410–4413. doi: 10.1128/jvi.62.11.4410-4413.1988

Transactivating potential of the 3' open reading frame of murine mammary tumor virus.

P van Klaveren 1, P Bentvelzen 1
PMCID: PMC253885  PMID: 2459415

Abstract

The procaryotic chloramphenicol acetyltransferase (CAT) gene controlled by the murine mammary tumor virus (MMTV) promoter shows reduced activity in a rat mammary tumor cell line after infection with MMTV but to a considerably lesser extent than the CAT gene controlled by a heterologous promoter, indicating trans-acting regulation of promoter activity by MMTV. Cotransfection of vectors capable of expressing RNA from the 3' open reading frame (orf) of MMTV with the CAT-MMTV construct resulted in enhanced CAT activity, suggesting that orf carries a transactivating potential. Since transactivation was also found with a vector containing only orf and part of the viral env gene, it was concluded that a separate transcriptional unit exists for the orf message.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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