Skip to main content
Journal of Virology logoLink to Journal of Virology
. 1987 Feb;61(2):597–599. doi: 10.1128/jvi.61.2.597-599.1987

Identification of the gene encoding an RNA polymerase-binding protein of bacteriophage T4.

K P Williams, G A Kassavetis, F S Esch, E P Geiduschek
PMCID: PMC253987  PMID: 3806793

Abstract

One of five bacteriophage T4-specified proteins that bind to host RNA polymerase core has been purified and partially sequenced. A mixed oligonucleotide, based on the amino acid sequence, was used to probe genomic restriction fragments. The gene for this protein, previously designated the 15K protein, has been located between T4 genes 45 and 46 and designated rpbA.

Full text

PDF
597

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Esch F. S., Ling N. C., Böhlen P. Microisolation of neuropeptides. Methods Enzymol. 1983;103:72–89. doi: 10.1016/s0076-6879(83)03007-4. [DOI] [PubMed] [Google Scholar]
  2. Esch F. S. Polypeptide microsequence analysis with the commercially available gas-phase sequencer. Anal Biochem. 1984 Jan;136(1):39–47. doi: 10.1016/0003-2697(84)90305-1. [DOI] [PubMed] [Google Scholar]
  3. Esch F., Baird A., Ling N., Ueno N., Hill F., Denoroy L., Klepper R., Gospodarowicz D., Böhlen P., Guillemin R. Primary structure of bovine pituitary basic fibroblast growth factor (FGF) and comparison with the amino-terminal sequence of bovine brain acidic FGF. Proc Natl Acad Sci U S A. 1985 Oct;82(19):6507–6511. doi: 10.1073/pnas.82.19.6507. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hercules K., Sauerbier W. Two modes of in vivo transcription for genes 43 and 45 of phage T4. J Virol. 1974 Aug;14(2):341–348. doi: 10.1128/jvi.14.2.341-348.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Horvitz H. R. Polypeptide bound to the host RNA polymerase is specified by T4 control gene 33. Nat New Biol. 1973 Aug 1;244(135):137–140. doi: 10.1038/newbio244137a0. [DOI] [PubMed] [Google Scholar]
  6. Hsu T., Wei R. X., Dawson M., Karam J. D. Identification of two new bacteriophage T4 genes that may have roles in transcription and DNA replication. J Virol. 1987 Feb;61(2):366–374. doi: 10.1128/jvi.61.2.366-374.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Jacobs K. A., Albright L. M., Shibata D. K., Geiduschek E. P. Genetic complementation by cloned bacteriophage T4 late genes. J Virol. 1981 Jul;39(1):31–45. doi: 10.1128/jvi.39.1.31-45.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kassavetis G. A., Geiduschek E. P. Defining a bacteriophage T4 late promoter: bacteriophage T4 gene 55 protein suffices for directing late promoter recognition. Proc Natl Acad Sci U S A. 1984 Aug;81(16):5101–5105. doi: 10.1073/pnas.81.16.5101. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kessler C., Neumaier P. S., Wolf W. Recognition sequences of restriction endonucleases and methylases--a review. Gene. 1985;33(1):1–102. doi: 10.1016/0378-1119(85)90119-2. [DOI] [PubMed] [Google Scholar]
  10. Malik S., Dimitrov M., Goldfarb A. Initiation of transcription by bacteriophage T4-modified RNA polymerase independently of host sigma factor. J Mol Biol. 1985 Sep 5;185(1):83–91. doi: 10.1016/0022-2836(85)90184-6. [DOI] [PubMed] [Google Scholar]
  11. Malik S., Goldfarb A. The effect of a bacteriophage T4-induced polypeptide on host RNA polymerase interaction with promoters. J Biol Chem. 1984 Nov 10;259(21):13292–13297. [PubMed] [Google Scholar]
  12. Ratner D. Letter to the editor: Bacteriophage T4 transcriptional control gene 55 codes for a protein bound to Escherichia coli RNA polymerase. J Mol Biol. 1974 Nov 15;89(4):803–807. doi: 10.1016/0022-2836(74)90054-0. [DOI] [PubMed] [Google Scholar]
  13. Ratner D. The interaction bacterial and phage proteins with immobilized Escherichia coli RNA polymerase. J Mol Biol. 1974 Sep 15;88(2):373–383. doi: 10.1016/0022-2836(74)90488-4. [DOI] [PubMed] [Google Scholar]
  14. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  15. Spicer E. K., Noble J. A., Nossal N. G., Konigsberg W. H., Williams K. R. Bacteriophage T4 gene 45. Sequences of the structural gene and its protein product. J Biol Chem. 1982 Aug 10;257(15):8972–8979. [PubMed] [Google Scholar]
  16. Stevens A. Deoxyribonucleic acid dependent ribonucleic acid polymerases from two T4 phage-infected systems. Biochemistry. 1974 Jan 29;13(3):493–503. doi: 10.1021/bi00700a015. [DOI] [PubMed] [Google Scholar]
  17. Stevens A. New small polypeptides associated with DNA-dependent RNA polymerase of Escherichia coli after infection with bacteriophage T4. Proc Natl Acad Sci U S A. 1972 Mar;69(3):603–607. doi: 10.1073/pnas.69.3.603. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES