Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1987 Mar;61(3):667–672. doi: 10.1128/jvi.61.3.667-672.1987

Unusual transcripts in La Crosse virus-infected cells and the site for nucleocapsid assembly.

R Raju, D Kolakofsky
PMCID: PMC254005  PMID: 3806796

Abstract

The La Crosse virus S genome segment is known to code for two plus-strand transcripts, free S-mRNA (nucleotide [nt] -15 to 886) and encapsidated antigenome RNA (nt 1 to 983). Early in infection only these plus-strand transcripts could be detected, but at later times plus-strand RNAs representing nt 1 to 886 in an encapsidated form and nt -15 to 983 as a free RNA could also be seen, as well as S-mRNA in an encapsidated form. The encapsidated S-mRNA became relatively important at later times because the free S-mRNA turned over rapidly after 5 h postinfection. The existence of these unusual RNAs and their presence as either free or encapsidated species suggests that the site for nucleocapsid assembly is located at the 5' ends of the genome and antigenome chains.

Full text

PDF
670

Images in this article

668Image
on p.668
669Image
on p.669
670Image
on p.670
671Image
on p.671
672Image
on p.672

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bellocq C., Raju R., Patterson J., Kolakofsky D. Translational requirement of La Crosse virus S-mRNA synthesis: in vitro studies. J Virol. 1987 Jan;61(1):87–95. doi: 10.1128/jvi.61.1.87-95.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bishop D. H., Gay M. E., Matsuoko Y. Nonviral heterogeneous sequences are present at the 5' ends of one species of snowshoe hare bunyavirus S complementary RNA. Nucleic Acids Res. 1983 Sep 24;11(18):6409–6418. doi: 10.1093/nar/11.18.6409. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Blumberg B. M., Giorgi C., Rose K., Kolakofsky D. Preparation and analysis of the nucleocapsid proteins of vesicular stomatitis virus and sendai virus, and analysis of the sendai virus leader-NP gene region. J Gen Virol. 1984 Apr;65(Pt 4):769–779. doi: 10.1099/0022-1317-65-4-769. [DOI] [PubMed] [Google Scholar]
  4. Bouloy M., Vialat P., Girard M., Pardigon N. A transcript from the S segment of the Germiston bunyavirus is uncapped and codes for the nucleoprotein and a nonstructural protein. J Virol. 1984 Mar;49(3):717–723. doi: 10.1128/jvi.49.3.717-723.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Chirgwin J. M., Przybyla A. E., MacDonald R. J., Rutter W. J. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry. 1979 Nov 27;18(24):5294–5299. doi: 10.1021/bi00591a005. [DOI] [PubMed] [Google Scholar]
  6. Grubman M. J., Shafritz D. A. Identification and characterization of messenger ribonucleoprotein complexes from vesicular stomatitis virus-infected HeLa cells. Virology. 1977 Aug;81(1):1–16. doi: 10.1016/0042-6822(77)90053-8. [DOI] [PubMed] [Google Scholar]
  7. Krug R. M. Priming of influenza viral RNA transcription by capped heterologous RNAs. Curr Top Microbiol Immunol. 1981;93:125–149. doi: 10.1007/978-3-642-68123-3_6. [DOI] [PubMed] [Google Scholar]
  8. Obijeski J. F., Bishop D. H., Palmer E. L., Murphy F. A. Segmented genome and nucleocapsid of La Crosse virus. J Virol. 1976 Dec;20(3):664–675. doi: 10.1128/jvi.20.3.664-675.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Obijeski J. F., Murphy F. A. Bunyaviridae: recent biochemical developments. J Gen Virol. 1977 Oct;37(1):1–14. doi: 10.1099/0022-1317-37-1-1. [DOI] [PubMed] [Google Scholar]
  10. Patterson J. L., Holloway B., Kolakofsky D. La Crosse virions contain a primer-stimulated RNA polymerase and a methylated cap-dependent endonuclease. J Virol. 1984 Oct;52(1):215–222. doi: 10.1128/jvi.52.1.215-222.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Patterson J. L., Kolakofsky D. Characterization of La Crosse virus small-genome transcripts. J Virol. 1984 Mar;49(3):680–685. doi: 10.1128/jvi.49.3.680-685.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Raju R., Kolakofsky D. Inhibitors of protein synthesis inhibit both La Crosse virus S-mRNA and S genome syntheses in vivo. Virus Res. 1986 Jul;5(1):1–9. doi: 10.1016/0168-1702(86)90061-4. [DOI] [PubMed] [Google Scholar]
  13. Rosen C. A., Ennis H. L., Cohen P. S. Translational control of vesicular stomatitis virus protein synthesis: isolation of an mRNA-sequestering particle. J Virol. 1982 Dec;44(3):932–938. doi: 10.1128/jvi.44.3.932-938.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Rossier C., Patterson J., Kolakofsky D. La Crosse virus small genome mRNA is made in the cytoplasm. J Virol. 1986 May;58(2):647–650. doi: 10.1128/jvi.58.2.647-650.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Sanger F., Coulson A. R. The use of thin acrylamide gels for DNA sequencing. FEBS Lett. 1978 Mar 1;87(1):107–110. doi: 10.1016/0014-5793(78)80145-8. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES