Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1987 Apr;61(4):1240–1243. doi: 10.1128/jvi.61.4.1240-1243.1987

Complete interaction of cellular 56,000- and 32,000-Mr proteins with simian virus 40 small-t antigen in productively infected cells.

K Rundell
PMCID: PMC254086  PMID: 3029419

Abstract

Two cellular proteins are found to be complexed with simian virus 40 small-t antigen in cellular extracts. The complex is a relatively unstable but dynamic one which can dissociate and reform in extracts. In extracts of permissive monkey kidney cells, the small-t antigen appeared to be present in excess, whereas the cellular proteins were nearly entirely committed to the complex in permissive monkey kidney cells.

Full text

PDF
1241

Images in this article

1241Image
on p.1241
1241Image
on p.1241
1242Image
on p.1242
1242Image
on p.1242

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bossert A., Mulgaonkar P., Rundell K. Interaction of simian virus 40 small-T antigen produced in bacteria with 56K and 32K proteins of animal cells. J Virol. 1985 Oct;56(1):325–327. doi: 10.1128/jvi.56.1.325-327.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bouck N., Beales N., Shenk T., Berg P., di Mayorca G. New region of the simian virus 40 genome required for efficient viral transformation. Proc Natl Acad Sci U S A. 1978 May;75(5):2473–2477. doi: 10.1073/pnas.75.5.2473. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Brugge J., Yonemoto W., Darrow D. Interaction between the Rous sarcoma virus transforming protein and two cellular phosphoproteins: analysis of the turnover and distribution of this complex. Mol Cell Biol. 1983 Jan;3(1):9–19. doi: 10.1128/mcb.3.1.9. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gilead Z., Jeng Y. H., Wold W. S., Sugawara K., Rho H. M., Harter M. L., Green M. Immunological identification of two adenovirus 2-induced early proteins possibly involved in cell transformation. Nature. 1976 Nov 18;264(5583):263–266. doi: 10.1038/264263a0. [DOI] [PubMed] [Google Scholar]
  5. Harlow E., Crawford L. V., Pim D. C., Williamson N. M. Monoclonal antibodies specific for simian virus 40 tumor antigens. J Virol. 1981 Sep;39(3):861–869. doi: 10.1128/jvi.39.3.861-869.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  7. Martin R. G., Setlow V. P., Edwards C. A., Vembu D. The roles of the simian virus 40 tumor antigens in transformation of Chinese hamster lung cells. Cell. 1979 Jul;17(3):635–643. doi: 10.1016/0092-8674(79)90271-x. [DOI] [PubMed] [Google Scholar]
  8. Renz C., Rundell K. Simian virus 40 small-t antigen-induced theophylline resistance is not mediated by cyclic AMP. J Virol. 1985 Jun;54(3):876–878. doi: 10.1128/jvi.54.3.876-878.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Rubin H., Figge J., Bladon M. T., Chen L. B., Ellman M., Bikel I., Farrell M., Livingston D. M. Role of small t antigen in the acute transforming activity of SV40. Cell. 1982 Sep;30(2):469–480. doi: 10.1016/0092-8674(82)90244-6. [DOI] [PubMed] [Google Scholar]
  10. Rundell K., Cox J. Simian virus 40 t antigen affects the sensitivity of cellular DNA synthesis to theophylline. J Virol. 1979 Apr;30(1):394–396. doi: 10.1128/jvi.30.1.394-396.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Rundell K., Major E. O., Lampert M. Association of cellular 56,000- and 32,000-molecular-weight protein with BK virus and polyoma virus t-antigens. J Virol. 1981 Mar;37(3):1090–1093. doi: 10.1128/jvi.37.3.1090-1093.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Rundell K. Presence in growth-arrested cells of cellular proteins that interact with simian virus 40 small-t antigen. J Virol. 1982 Jun;42(3):1135–1137. doi: 10.1128/jvi.42.3.1135-1137.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Rundell K., Yang Y. C. SV40 antitumor sera that directly immunoprecipitate small-t-associated cellular proteins. Virology. 1981 Oct 15;114(1):297–301. doi: 10.1016/0042-6822(81)90281-6. [DOI] [PubMed] [Google Scholar]
  14. Schaffhausen B. S., Silver J. E., Benjamin T. L. Tumor antigen(s) in cell productively infected by wild-type polyoma virus and mutant NG-18. Proc Natl Acad Sci U S A. 1978 Jan;75(1):79–83. doi: 10.1073/pnas.75.1.79. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Shenk T. E., Carbon J., Berg P. Construction and analysis of viable deletion mutants of simian virus 40. J Virol. 1976 May;18(2):664–671. doi: 10.1128/jvi.18.2.664-671.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Sleigh M. J., Topp W. C., Hanich R., Sambrook J. F. Mutants of SV40 with an altered small t protein are reduced in their ability to transform cells. Cell. 1978 May;14(1):79–88. doi: 10.1016/0092-8674(78)90303-3. [DOI] [PubMed] [Google Scholar]
  17. Yang Y. C., Hearing P., Rundell K. Cellular proteins associated with simian virus 40 early gene products in newly infected cells. J Virol. 1979 Oct;32(1):147–154. doi: 10.1128/jvi.32.1.147-154.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES