Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1985 Feb;53(2):672–678. doi: 10.1128/jvi.53.2.672-678.1985

Adenovirus 3 fiber polypeptide gene: implications for the structure of the fiber protein.

C Signäs, G Akusjärvi, U Pettersson
PMCID: PMC254684  PMID: 2982041

Abstract

The nucleotide sequence of a 1,330-base-pair-long DNA segment located between map coordinates 88.5 and 92.3 in the adenovirus type 3 (Ad3) genome was determined. Transcripts from the r-strand of the region were mapped by S1 nuclease analysis and by in vitro translation of RNA, selected by filter hybridization. The results revealed that the sequenced region encodes the Ad3 fiber polypeptide with a molecular weight of 34,800. A comparison between the predicted amino acid sequences of the Ad3 and the Ad2 fiber polypeptides revealed that they have almost identical secondary structures, consisting of a tail, a shaft, and a knob. A striking difference between Ad2 and Ad3 fibers was that the shaft of the Ad3 fiber was significantly shorter, containing only 6 repeat units compared with 22 in the Ad2 fiber. The secondary structure suggests that the fiber is a dimeric structure, as proposed earlier (N.M. Green, N.G. Wrigley, W.C. Russell, S.R. Martin, and A.D. McLachlan, EMBO J. 2:1357-1365, 1983), with the size of the polypeptide determining the length of the fiber protein.

Full text

PDF
677

Images in this article

676Image
on p.676
676Image
on p.676

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Berk A. J., Sharp P. A. Structure of the adenovirus 2 early mRNAs. Cell. 1978 Jul;14(3):695–711. doi: 10.1016/0092-8674(78)90252-0. [DOI] [PubMed] [Google Scholar]
  2. Boudin M. L., Boulanger P. Assembly of adenovirus penton base and fiber. Virology. 1982 Jan 30;116(2):589–604. doi: 10.1016/0042-6822(82)90151-9. [DOI] [PubMed] [Google Scholar]
  3. Chee-Sheung C. C., Ginsberg H. S. Characterization of a temperature-sensitive fiber mutant of type 5 adenovirus and effect of the mutation on virion assembly. J Virol. 1982 Jun;42(3):932–950. doi: 10.1128/jvi.42.3.932-950.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Devaux C., Zulauf M., Boulanger P., Jacrot B. Molecular weight of adenovirus serotype 2 capsomers. A new characterization. J Mol Biol. 1982 Apr 25;156(4):927–939. doi: 10.1016/0022-2836(82)90148-6. [DOI] [PubMed] [Google Scholar]
  5. Dorsett P. H., G8nsberg H. S. Characterization of type 5 adenovirus fiber protein. J Virol. 1975 Jan;15(1):208–216. doi: 10.1128/jvi.15.1.208-216.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Edvardsson B., Everitt E., Jörnvall H., Prage L., Philipson L. Intermediates in adenovirus assembly. J Virol. 1976 Aug;19(2):533–547. doi: 10.1128/jvi.19.2.533-547.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gingeras T. R., Sciaky D., Gelinas R. E., Bing-Dong J., Yen C. E., Kelly M. M., Bullock P. A., Parsons B. L., O'Neill K. E., Roberts R. J. Nucleotide sequences from the adenovirus-2 genome. J Biol Chem. 1982 Nov 25;257(22):13475–13491. [PubMed] [Google Scholar]
  8. Green N. M., Wrigley N. G., Russell W. C., Martin S. R., McLachlan A. D. Evidence for a repeating cross-beta sheet structure in the adenovirus fibre. EMBO J. 1983;2(8):1357–1365. doi: 10.1002/j.1460-2075.1983.tb01592.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Grunstein M., Hogness D. S. Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene. Proc Natl Acad Sci U S A. 1975 Oct;72(10):3961–3965. doi: 10.1073/pnas.72.10.3961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hérissé J., Galibert F. Nucleotide sequence of the EcoRI E fragment of adenovirus 2 genome. Nucleic Acids Res. 1981 Mar 11;9(5):1229–1240. doi: 10.1093/nar/9.5.1229. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hérissé J., Rigolet M., de Dinechin S. D., Galibert F. Nucleotide sequence of adenovirus 2 DNA fragment encoding for the carboxylic region of the fiber protein and the entire E4 region. Nucleic Acids Res. 1981 Aug 25;9(16):4023–4042. doi: 10.1093/nar/9.16.4023. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Ishibashi M., Maizel J. V., Jr The polypeptides of adenovirus. VI. Early and late glycopolypeptides. Virology. 1974 Apr;58(2):345–361. doi: 10.1016/0042-6822(74)90070-1. [DOI] [PubMed] [Google Scholar]
  13. Kilpatrick B. A., Gelinas R. E., Broker T. R., Chow L. T. Comparison of late mRNA splicing among class B and class C adenoviruses. J Virol. 1979 Jun;30(3):899–912. doi: 10.1128/jvi.30.3.899-912.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Le Moullec J. M., Akusjärvi G., Stålhandske P., Pettersson U., Chambraud B., Gilardi P., Nasri M., Perricaudet M. Polyadenylic acid addition sites in the adenovirus type 2 major late transcription unit. J Virol. 1983 Oct;48(1):127–134. doi: 10.1128/jvi.48.1.127-134.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Maizel J. V., Jr, White D. O., Scharff M. D. The polypeptides of adenovirus. II. Soluble proteins, cores, top components and the structure of the virion. Virology. 1968 Sep;36(1):126–136. doi: 10.1016/0042-6822(68)90122-0. [DOI] [PubMed] [Google Scholar]
  16. Maxam A. M., Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. doi: 10.1016/s0076-6879(80)65059-9. [DOI] [PubMed] [Google Scholar]
  17. McGrogan M., Spector D. J., Goldenberg C. J., Halbert D., Raskas H. J. Purification of specific adenovirus 2 RNAs by preparative hybridization and selective thermal elution. Nucleic Acids Res. 1979 Feb;6(2):593–607. doi: 10.1093/nar/6.2.583. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Norrby E. The relationship between the soluble antigens and the virion of adenovirus type 3. IV. Immunological complexity of soluble components. Virology. 1969 Apr;37(4):565–576. doi: 10.1016/0042-6822(69)90274-8. [DOI] [PubMed] [Google Scholar]
  19. Norrby E. The structural and functional diversity of Adenovirus capsid components. J Gen Virol. 1969 Sep;5(2):221–236. doi: 10.1099/0022-1317-5-2-221. [DOI] [PubMed] [Google Scholar]
  20. Pettersson U., Sambrook J. Amount of viral DNA in the genome of cells transformed by adenovirus type 2. J Mol Biol. 1973 Jan;73(1):125–130. doi: 10.1016/0022-2836(73)90164-2. [DOI] [PubMed] [Google Scholar]
  21. Pettersson U., Tibbetts C., Philipson L. Hybridization maps of early and late messenger RNA sequences on the adenovirus type 2 genome. J Mol Biol. 1976 Mar 15;101(4):479–501. doi: 10.1016/0022-2836(76)90241-2. [DOI] [PubMed] [Google Scholar]
  22. Sanger F., Coulson A. R. The use of thin acrylamide gels for DNA sequencing. FEBS Lett. 1978 Mar 1;87(1):107–110. doi: 10.1016/0014-5793(78)80145-8. [DOI] [PubMed] [Google Scholar]
  23. Stålhandske P., Persson H., Perricaudet M., Philipson L., Pettersson U. Structure of three spliced mRNAs from region E3 of adenovirus type 2. Gene. 1983 May-Jun;22(2-3):157–165. doi: 10.1016/0378-1119(83)90099-9. [DOI] [PubMed] [Google Scholar]
  24. Sunquist B., Pettersson U., Thelander L., Philipson L. Structural proteins of adenoviruses. IX. Molecular weight and subunit composition of adenovirus type 2 fiber. Virology. 1973 Jan;51(1):252–256. doi: 10.1016/0042-6822(73)90389-9. [DOI] [PubMed] [Google Scholar]
  25. Svensson C., Pettersson U., Akusjärvi G. Splicing of adenovirus 2 early region 1A mRNAs is non-sequential. J Mol Biol. 1983 Apr 15;165(3):475–495. doi: 10.1016/s0022-2836(83)80214-9. [DOI] [PubMed] [Google Scholar]
  26. Tanaka T., Weisblum B. Construction of a colicin E1-R factor composite plasmid in vitro: means for amplification of deoxyribonucleic acid. J Bacteriol. 1975 Jan;121(1):354–362. doi: 10.1128/jb.121.1.354-362.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Tibbetts C. Physical organization of subgroup B human adenovirus genomes. J Virol. 1977 Nov;24(2):564–579. doi: 10.1128/jvi.24.2.564-579.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Wadell G. Classification of human adenoviruses by SDS-polyacrylamide gel electrophoresis of structural polypeptides. Intervirology. 1979;11(1):47–57. doi: 10.1159/000149011. [DOI] [PubMed] [Google Scholar]
  29. Weaver R. F., Weissmann C. Mapping of RNA by a modification of the Berk-Sharp procedure: the 5' termini of 15 S beta-globin mRNA precursor and mature 10 s beta-globin mRNA have identical map coordinates. Nucleic Acids Res. 1979 Nov 10;7(5):1175–1193. doi: 10.1093/nar/7.5.1175. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES