Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1985 Apr;54(1):219–223. doi: 10.1128/jvi.54.1.219-223.1985

Persistence of echovirus 6 in cloned human cells.

J P Gibson, V F Righthand
PMCID: PMC254780  PMID: 3973978

Abstract

Establishment of a persistent infection by echovirus 6 in cloned human WISH cells (PI) was demonstrated. The cloned human WISH cells were maintained for 3.0 years (over 125 passages) and released virus continuously without cellular destruction. Neither temperature-sensitive virus mutants nor interferon appears to play a role in either establishment or maintenance of viral persistence. The majority of the virus produced by cloned human WISH cells is defective (2 X 10(6) particles per PFU) and differs from standard virus in its polypeptide profile and its inability to attach to parental WISH cells.

Full text

PDF
222

Images in this article

220Image
on p.220
221Image
on p.221

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ACKERMANN W. W., KURTZ H. Observations concerning a persisting infection of HeLa cells with poliomyelitis virus. J Exp Med. 1955 Nov 1;102(5):555–565. doi: 10.1084/jem.102.5.555. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BARRON A. L., KARZON D. T. STUDIES OF MUTANTS OF ECHOVIRUS 6. I. BIOLOGIC AND SEROLOGIC CHARACTERISTICS. Am J Epidemiol. 1965 May;81:323–332. doi: 10.1093/oxfordjournals.aje.a120518. [DOI] [PubMed] [Google Scholar]
  3. CROWELL R. L., SYVERTON J. T. The mammalian cell-virus relationship. VI. Sustained infection of HeLa cells by Coxsackie B3 virus and effect on superinfection. J Exp Med. 1961 Feb 1;113:419–435. doi: 10.1084/jem.113.2.419. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Davis L. E., Bodian D., Price D., Butler I. J., Vickers J. H. Chronic progressive poliomyelitis secondary to vaccination of an immunodeficient child. N Engl J Med. 1977 Aug 4;297(5):241–245. doi: 10.1056/NEJM197708042970503. [DOI] [PubMed] [Google Scholar]
  5. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  6. Oakley B. R., Kirsch D. R., Morris N. R. A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels. Anal Biochem. 1980 Jul 1;105(2):361–363. doi: 10.1016/0003-2697(80)90470-4. [DOI] [PubMed] [Google Scholar]
  7. PACSA S. Poliovirus-carrying lines of HeLa cells: their establishment and sensitivity to viruses. Acta Microbiol Acad Sci Hung. 1961;8:329–332. [PubMed] [Google Scholar]
  8. Puck T. T., Marcus P. I. A RAPID METHOD FOR VIABLE CELL TITRATION AND CLONE PRODUCTION WITH HELA CELLS IN TISSUE CULTURE: THE USE OF X-IRRADIATED CELLS TO SUPPLY CONDITIONING FACTORS. Proc Natl Acad Sci U S A. 1955 Jul 15;41(7):432–437. doi: 10.1073/pnas.41.7.432. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Righthand F. In vitro interactions between virus and enhancer. Infect Immun. 1972 Nov;6(5):805–809. doi: 10.1128/iai.6.5.805-809.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. TAKEMOTO K. K., HABEL K. Virus-cell relationship in a carrier culture of HeLa cells and Coxsackie A9 virus. Virology. 1959 Jan;7(1):28–44. doi: 10.1016/0042-6822(59)90175-8. [DOI] [PubMed] [Google Scholar]
  11. VOGT M., DULBECCO R. Properties of a HeLa cell culture with increased resistance to poliomyelitis virus. Virology. 1958 Jun;5(3):425–434. doi: 10.1016/0042-6822(58)90037-0. [DOI] [PubMed] [Google Scholar]
  12. Vallbracht A., Hofmann L., Wurster K. G., Flehmig B. Persistent infection of human fibroblasts by hepatitis A virus. J Gen Virol. 1984 Mar;65(Pt 3):609–615. doi: 10.1099/0022-1317-65-3-609. [DOI] [PubMed] [Google Scholar]
  13. WALKER D. L. THE VIRAL CARRIER STATE IN ANIMAL CELL CULTURES. Prog Med Virol. 1964;6:111–148. [PubMed] [Google Scholar]
  14. Wilfert C. M., Buckley R. H., Mohanakumar T., Griffith J. F., Katz S. L., Whisnant J. K., Eggleston P. A., Moore M., Treadwell E., Oxman M. N. Persistent and fatal central-nervous-system ECHOvirus infections in patients with agammaglobulinemia. N Engl J Med. 1977 Jun 30;296(26):1485–1489. doi: 10.1056/NEJM197706302962601. [DOI] [PubMed] [Google Scholar]
  15. Wyatt H. V. Poliomyelitis in hypogammaglobulinemics. J Infect Dis. 1973 Dec;128(6):802–806. doi: 10.1093/infdis/128.6.802. [DOI] [PubMed] [Google Scholar]
  16. Yoneyama T., Hagiwara A., Hara M., Shimojo H. Alteration in oligonucleotide fingerprint patterns of the viral genome in poliovirus type 2 isolated from paralytic patients. Infect Immun. 1982 Jul;37(1):46–53. doi: 10.1128/iai.37.1.46-53.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES