Skip to main content
Journal of Virology logoLink to Journal of Virology
. 1983 Apr;46(1):137–142. doi: 10.1128/jvi.46.1.137-142.1983

Structural and Intracellular Proteins of the Nonoccluded Baculovirus HZ-1

John P Burand 1, Brad Stiles 1, H Alan Wood 1
PMCID: PMC255101  PMID: 16789238

Abstract

A plaque-purified isolate of the baculovirus HZ-1 was used to examine the kinetics of replication of this persistent, nonoccluded virus in TN-368 cells. Twenty-eight virus structural proteins ranging in molecular weight from 153,000 to 14,000 were identified. Fourteen of these proteins were found to be glycosylated. The sequence of appearance of the 37 virus-induced intracellular polypeptides was determined by pulse-labeling with [35S]methionine. N-[3H]acetylglucosamine, [3H]mannose, and the glycosylation inhibitor tunicamycin were used to detect virus structural glycoproteins. Post-transcriptional modification of two virus-induced proteins was detected.

Full text

PDF
137

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Butters T. D., Hughes R. C., Vischer P. Steps in the biosynthesis of mosquito cell membrane glycoproteins and the effects of tunicamycin. Biochim Biophys Acta. 1981 Feb 6;640(3):672–686. doi: 10.1016/0005-2736(81)90097-3. [DOI] [PubMed] [Google Scholar]
  2. Carstens E. B., Tjia S. T., Doerfler W. Infection of Spodoptera frugiperda cells with Autographa californica nuclear polyhedrosis virus I. Synthesis of intracellular proteins after virus infection. Virology. 1979 Dec;99(2):386–398. doi: 10.1016/0042-6822(79)90017-5. [DOI] [PubMed] [Google Scholar]
  3. Granados R. R., Nguyen T., Cato B. An insect cell line persistently infected with a baculovirus-like particle. Intervirology. 1978;10(5):309–317. doi: 10.1159/000148993. [DOI] [PubMed] [Google Scholar]
  4. Hink W. F. Established insect cell line from the cabbage looper, Trichoplusia ni. Nature. 1970 May 2;226(5244):466–467. doi: 10.1038/226466b0. [DOI] [PubMed] [Google Scholar]
  5. Huang Y. S., Hedberg M., Kawanishi C. Y. Characterization of the DNA of a Nonoccluded Baculovirus, Hz-1V. J Virol. 1982 Jul;43(1):174–181. doi: 10.1128/jvi.43.1.174-181.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  7. Laskey R. A. The use of intensifying screens or organic scintillators for visualizing radioactive molecules resolved by gel electrophoresis. Methods Enzymol. 1980;65(1):363–371. doi: 10.1016/s0076-6879(80)65047-2. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES