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. 1983 May;46(2):632–637. doi: 10.1128/jvi.46.2.632-637.1983

Isolation of a nondefective recombinant between adenovirus type 5 and early region 1A of adenovirus type 12.

K Shiroki, I Saito, K Maruyama, H Shimojo
PMCID: PMC255168  PMID: 6842682

Abstract

A nondefective recombinant between adenovirus type 5 (Ad5) and type 12 (Ad12), rc-1 (Ad5 dl312, carrying the Ad12 E1A gene), was isolated from hamster cell foci transformed by a defective recombinant, rcB-1 (dl312, carrying the Ad12 E1 gene). The recombinant rc-1 grew in human embryo kidney and KB cells in the absence of helper and synthesized Ad12 T antigen g, the product of the E1A gene. The genome of rc-1 has a deletion between 79.9 and 82.5 map units of Ad5 dl312 DNA with an insertion of 0.1 to 5.5 map units of Ad12 DNA at the deletion site. The mRNAs of Ad12 E1A were transcribed from the Ad12 E1A promoter, and unusual RNAs were abundantly transcribed from the Ad5 E3 promoter on the opposite strand. The frequency of cell transformation with rc-1 was lower than those with Ad5 and Ad12 wild types.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Berk A. J., Sharp P. A. Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids. Cell. 1977 Nov;12(3):721–732. doi: 10.1016/0092-8674(77)90272-0. [DOI] [PubMed] [Google Scholar]
  2. Bernards R., Houweling A., Schrier P. I., Bos J. L., Van der Eb A. J. Characterization of cells transformed by Ad5/Ad12 hybrid early region I plasmids. Virology. 1982 Jul 30;120(2):422–432. doi: 10.1016/0042-6822(82)90042-3. [DOI] [PubMed] [Google Scholar]
  3. Brusca J. S., Chinnadurai G. Transforming genes among three different oncogenic subgroups of human adenoviruses have similar replicative functions. J Virol. 1981 Jul;39(1):300–305. doi: 10.1128/jvi.39.1.300-305.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chow L. T., Roberts J. M., Lewis J. B., Broker T. R. A map of cytoplasmic RNA transcripts from lytic adenovirus type 2, determined by electron microscopy of RNA:DNA hybrids. Cell. 1977 Aug;11(4):819–836. doi: 10.1016/0092-8674(77)90294-x. [DOI] [PubMed] [Google Scholar]
  5. Freeman A. E., Black P. H., Wolford R., Huebner R. J. Adenovirus type 12-rat embryo transformation system. J Virol. 1967 Apr;1(2):362–367. doi: 10.1128/jvi.1.2.362-367.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gallimore P. H., Paraskeva C. A study to determine the reasons for differences in the tumorigenicity of rat cell lines transformed by adenovirus 2 and adenovirus 12. Cold Spring Harb Symp Quant Biol. 1980;44(Pt 1):703–713. doi: 10.1101/sqb.1980.044.01.075. [DOI] [PubMed] [Google Scholar]
  7. Graham F. L., Smiley J., Russell W. C., Nairn R. Characteristics of a human cell line transformed by DNA from human adenovirus type 5. J Gen Virol. 1977 Jul;36(1):59–74. doi: 10.1099/0022-1317-36-1-59. [DOI] [PubMed] [Google Scholar]
  8. Jones N., Shenk T. Isolation of adenovirus type 5 host range deletion mutants defective for transformation of rat embryo cells. Cell. 1979 Jul;17(3):683–689. doi: 10.1016/0092-8674(79)90275-7. [DOI] [PubMed] [Google Scholar]
  9. Kimura G., Itagaki A., Summers J. Rat cell line 3y1 and its virogenic polyoma- and sv40- transformed derivatives. Int J Cancer. 1975 Apr 15;15(4):694–706. doi: 10.1002/ijc.2910150419. [DOI] [PubMed] [Google Scholar]
  10. Lewis A. M., Jr, Levin M. J., Wiese W. H., Crumpacker C. S., Henry P. H. A nondefective (competent) adenovirus-SV40 hybrid isolated from the AD.2-SV40 hybrid population. Proc Natl Acad Sci U S A. 1969 Aug;63(4):1128–1135. doi: 10.1073/pnas.63.4.1128. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Maruyama K., Hiwasa T., Oda K. I. Characterization of flat revertant cells isolated from simian virus 40-transformed mouse and rat cells which contain multiple copies of viral genomes. J Virol. 1981 Mar;37(3):1028–1043. doi: 10.1128/jvi.37.3.1028-1043.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Rowe D. T., Graham F. L. Complementation of adenovirus type 5 host range mutants by adenovirus type 12 in coinfected HeLa and BHK-21 cells. J Virol. 1981 Apr;38(1):191–197. doi: 10.1128/jvi.38.1.191-197.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Saito I., Sato J., Handa H., Shiroki K., Shimojo H. Mapping of RNAs transcribed from adenovirus type 12 early and VA RNA regions. Virology. 1981 Oct 30;114(2):379–398. doi: 10.1016/0042-6822(81)90219-1. [DOI] [PubMed] [Google Scholar]
  14. Segawa K., Saito I., Shiroki K., Shimojo H. In vitro translation of adenovirus type 12-specific mRNA complementary to the transforming gene. Virology. 1980 Nov;107(1):61–70. doi: 10.1016/0042-6822(80)90272-x. [DOI] [PubMed] [Google Scholar]
  15. Shiroki K., Maruyama K., Saito I., Fukui Y., Yazaki K., Shimojo H. Dependence of tumor-forming capacities of cells transformed by recombinants between adenovirus types 5 and 12 on expression of early region 1. J Virol. 1982 May;42(2):708–718. doi: 10.1128/jvi.42.2.708-718.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Shiroki K., Saito I., Maruyama K., Fukui Y., Imatani Y., Oda K. I., Shimojo H. Expression of adenovirus type 12 early region 1 in KB cells transformed by recombinants containing the gene. J Virol. 1983 Mar;45(3):1074–1082. doi: 10.1128/jvi.45.3.1074-1082.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  18. TRENTIN J. J., YABE Y., TAYLOR G. The quest for human cancer viruses. Science. 1962 Sep 14;137(3533):835–841. doi: 10.1126/science.137.3533.835. [DOI] [PubMed] [Google Scholar]
  19. Williams J., Ho Y. S., Galos R. Evidence for functional relatedness of products encoded by the transforming sequences of human adenovirus types 5 and 12. Virology. 1981 Apr 15;110(1):208–212. doi: 10.1016/0042-6822(81)90023-4. [DOI] [PubMed] [Google Scholar]

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