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. 1987 Jan;61(1):113–118. doi: 10.1128/jvi.61.1.113-118.1987

Prohead core of bacteriophage T4 can act as an intermediate in the T4 head assembly pathway.

A Kuhn, B Keller, M Maeder, F Traub
PMCID: PMC255215  PMID: 3537341

Abstract

Bacteriophage T4 assembly was impaired in Escherichia coli hdB3-1 at an incubation temperature below 30 degrees C. Naked prohead cores (head scaffold) bound to the inner surface of the plasma membrane accumulated, and the major shell protein (gp23) precipitated into visible intracellular aggregates in the cytoplasm. Shifting the temperature to 42 degrees C allowed newly synthesized gp23 to assemble around the accumulated cores. We conclude that synchronous assembly of the scaffold and shell is not obligatory and that naked cores can serve as intermediates in the T4 assembly pathway.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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