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. 1983 Nov;48(2):534–541. doi: 10.1128/jvi.48.2.534-541.1983

Major polypeptide of duck hepatitis B surface antigen particles.

P L Marion, S S Knight, M A Feitelson, L S Oshiro, W S Robinson
PMCID: PMC255377  PMID: 6620467

Abstract

The 40- to 50-nm pleomorphic particles found in the sera of domestic Pekin ducks infected with duck hepatitis B virus were purified by rate zonal and isopycnic centrifugation. Sodium dodecyl sulfate-polyacrylamide gel electrophoretic polypeptide analysis of these particles, called duck hepatitis B surface antigen particles, revealed the major component to be a single 17,500-dalton polypeptide. This result is in contrast to polypeptide analyses of the surface antigens of related mammalian viruses, including hepatitis B, in which a major doublet of polypeptides is seen with molecular weights ranging from 23,000 to 29,000. Tryptic maps of 17,500-dalton polypeptide resembled that of the major non-glycosylated polypeptide of the adw subtype of hepatitis B surface antigen. A serological assay for antibody to the purified duck virus particles is also described.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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