Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1983 Dec;48(3):779–784. doi: 10.1128/jvi.48.3.779-784.1983

Nucleotide sequences of gag-pol regions that determine the Fv-1 host range property of BALB/c N-tropic and B-tropic murine leukemia viruses.

C Y Ou, L R Boone, C K Koh, R W Tennant, W K Yang
PMCID: PMC255411  PMID: 6313971

Abstract

Previously, in vitro recombinant DNA studies demonstrated that genetic determinants of N-tropism and B-tropism, or Fv-1-related host range properties of murine leukemia viruses, were located in a BamHI-HindIII DNA segment derived from the 5' portion of the cloned viral genome. We sequenced this segment and its immediate 5' region from cloned DNA of two BALB/c mouse C-type viruses (WN1802N and WN1802B) and found base differences at 12 positions out of the otherwise identical 1,390-base-pair sequences. Analysis of the most likely reading frame showed that 6 of the 12 base differences would result in four encoded amino acid changes, three of which occur at positions 109 (glutamine in WN1802N versus threonine in WN1802B), 110 (arginine in WN1802N versus glutamic acid in WN1802B), and 159 (glutamic acid in WN1802N versus glycine in WN1802B) of the p30 protein. The remaining one is located at position 36 (threonine in WN1802N versus isoleucine in WN1802B) of the viral polymerase protein. Significant conformational alteration of the p30 protein could be predicted from these amino acid changes.

Full text

PDF
784

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Benade L. E., Barbacid M. Polymorphism among the major core proteins of C57BL B-tropic murine leukemia viruses. Virology. 1980 Oct 30;106(2):387–390. doi: 10.1016/0042-6822(80)90261-5. [DOI] [PubMed] [Google Scholar]
  2. Benade L. E., Ihle J. N., Declève A. Serological characterization of B-tropic viruses of C57BL mice: possible origin by recombination of endogenous N-tropic and xenotropic viruses. Proc Natl Acad Sci U S A. 1978 Sep;75(9):4553–4557. doi: 10.1073/pnas.75.9.4553. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Boone L. R., Myer F. E., Yang D. M., Kiggans J. O., Koh C., Tennant R. W., Yang W. K. Analysis of recombinant DNA clones of the endogenous BALB/c murine leukemia virus WN1802N: variation in long terminal repeat length. J Virol. 1983 Jan;45(1):484–488. doi: 10.1128/jvi.45.1.484-488.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Boone L. R., Myer F. E., Yang D. M., Ou C. Y., Koh C. K., Roberson L. E., Tennant R. W., Yang W. K. Reversal of Fv-1 host range by in vitro restriction endonuclease fragment exchange between molecular clones of N-tropic and B-tropic murine leukemia virus genomes. J Virol. 1983 Oct;48(1):110–119. doi: 10.1128/jvi.48.1.110-119.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Buchhagen D. L., Stutman O., Fleissner E. Chromatographic Separation and Antigenic Analysis of Proteins of the Oncornaviruses IV. Biochemical Typing of Murine Viral Proteins. J Virol. 1975 May;15(5):1148–1157. doi: 10.1128/jvi.15.5.1148-1157.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Chinsky J., Soeiro R. Fv-1 host restriction of Friend leukemia virus: analysis of unintegrated proviral DNA. J Virol. 1981 Oct;40(1):45–55. doi: 10.1128/jvi.40.1.45-55.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Chou P. Y., Fasman G. D. Prediction of protein conformation. Biochemistry. 1974 Jan 15;13(2):222–245. doi: 10.1021/bi00699a002. [DOI] [PubMed] [Google Scholar]
  8. Declève A., Niwa O., Gelmann E., Kaplan H. S. Replication kinetics of N- and B-tropic murine leukemia viruses on permissive and nonpermissive cells in vitro. Virology. 1975 Jun;65(2):320–332. doi: 10.1016/0042-6822(75)90038-0. [DOI] [PubMed] [Google Scholar]
  9. Faller D. V., Hopkins N. RNase T1-resistant oligonucleotides of B-tropic murine leukemia virus from BALB/c and five of its NB-tropic derivatives. J Virol. 1977 Jul;23(1):188–195. doi: 10.1128/jvi.23.1.188-195.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Faller D. V., Hopkins N. T1 oligonucleotide maps of N-, B-, and B leads to NB-tropic murine leukemia viruses derived from BALB/c. J Virol. 1978 Apr;26(1):143–152. doi: 10.1128/jvi.26.1.143-152.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Gautsch J. W., Elder J. H., Jensen F. C., Lerner R. A. In vitro construction of a B-tropic virus by recombination: B-tropism is a cryptic phenotype of xenotropic murine retroviruses. Proc Natl Acad Sci U S A. 1980 May;77(5):2989–2993. doi: 10.1073/pnas.77.5.2989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Gautsch J. W., Elder J. H., Schindler J., Jensen F. C., Lerner R. A. Structural markers on core protein p30 of murine leukemia virus: functional correlation with Fv-1 tropism. Proc Natl Acad Sci U S A. 1978 Sep;75(9):4170–4174. doi: 10.1073/pnas.75.9.4170. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hartley J. W., Rowe W. P., Capps W. I., Huebner R. J. Isolation of naturally occurring viruses of the murine leukemia virus group in tissue culture. J Virol. 1969 Feb;3(2):126–132. doi: 10.1128/jvi.3.2.126-132.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Hartley J. W., Rowe W. P., Huebner R. J. Host-range restrictions of murine leukemia viruses in mouse embryo cell cultures. J Virol. 1970 Feb;5(2):221–225. doi: 10.1128/jvi.5.2.221-225.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Hopkins N., Schindler J., Hynes R. Six-NB-tropic murine leukemia viruses derived from a B-tropic virus of BALB/c have altered p30. J Virol. 1977 Jan;21(1):309–318. doi: 10.1128/jvi.21.1.309-318.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Hopp T. P., Woods K. R. Prediction of protein antigenic determinants from amino acid sequences. Proc Natl Acad Sci U S A. 1981 Jun;78(6):3824–3828. doi: 10.1073/pnas.78.6.3824. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Jolicoeur P., Baltimore D. Effect of Fv-1 gene product on proviral DNA formation and integration in cells infected with murine leukemia viruses. Proc Natl Acad Sci U S A. 1976 Jul;73(7):2236–2240. doi: 10.1073/pnas.73.7.2236. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Jolicoeur P., Rassart E. Effect of Fv-1 gene product on synthesis of linear and supercoiled viral DNA in cells infected with murine leukemia virus. J Virol. 1980 Jan;33(1):183–195. doi: 10.1128/jvi.33.1.183-195.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Liou R. S., Boone L. R., Kiggans J. O., Yang D. M., Wang T. W., Tennant R. W., Yang W. K. Molecular cloning and analysis of the endogenous retrovirus chemically induced from RFM/Un mouse cell cultures. J Virol. 1983 Apr;46(1):288–292. doi: 10.1128/jvi.46.1.288-292.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. MOLONEY J. B. Biological studies on a lymphoid-leukemia virus extracted from sarcoma 37. I. Origin and introductory investigations. J Natl Cancer Inst. 1960 Apr;24:933–951. [PubMed] [Google Scholar]
  21. Maxam A. M., Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. doi: 10.1016/s0076-6879(80)65059-9. [DOI] [PubMed] [Google Scholar]
  22. Pincus T., Hartley J. W., Rowe W. P. A major genetic locus affecting resistance to infection with murine leukemia viruses. I. Tissue culture studies of naturally occurring viruses. J Exp Med. 1971 Jun 1;133(6):1219–1233. doi: 10.1084/jem.133.6.1219. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Pincus T., Hartley J. W., Rowe W. P. A major genetic locus affecting resistance to infection with murine leukemia viruses. IV. Dose-response relationships in Fv-1-sensitive and resistant cell cultures. Virology. 1975 Jun;65(2):333–342. doi: 10.1016/0042-6822(75)90039-2. [DOI] [PubMed] [Google Scholar]
  24. Pincus T., Rowe W. P., Lilly F. A major genetic locus affecting resistance to infection with murine leukemia viruses. II. Apparent identity to a major locus described for resistance to friend murine leukemia virus. J Exp Med. 1971 Jun 1;133(6):1234–1241. doi: 10.1084/jem.133.6.1234. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. RAUSCHER F. J. A virus-induced disease of mice characterized by erythrocytopoiesis and lymphoid leukemia. J Natl Cancer Inst. 1962 Sep;29:515–543. [PubMed] [Google Scholar]
  26. Reddy E. P., Smith M. J., Aaronson S. A. Complete nucleotide sequence and organization of the Moloney murine sarcoma virus genome. Science. 1981 Oct 23;214(4519):445–450. doi: 10.1126/science.6170110. [DOI] [PubMed] [Google Scholar]
  27. Rein A., Kashmiri S. V., Bassin R. H., Gerwin B. L., Duran-Troise G. Phenotypic mixing between N- and B-tropic murine leukemia viruses: infectious particles with dual sensitivity to Fv-1 restriction. Cell. 1976 Mar;7(3):373–379. doi: 10.1016/0092-8674(76)90166-5. [DOI] [PubMed] [Google Scholar]
  28. Robbins K. C., Cabradilla C. D., Stephenson J. R., Aaronson S. A. Segregation of genetic information for a B-tropic leukemia virus with the structural locus for BALB:virus-1. Proc Natl Acad Sci U S A. 1977 Jul;74(7):2953–2957. doi: 10.1073/pnas.74.7.2953. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Rommelaere J., Donis-Keller H., Hopkins N. RNA sequencing provides evidence for allelism of determinants of the N-, B- or NB-tropism of murine leukemia viruses. Cell. 1979 Jan;16(1):43–50. doi: 10.1016/0092-8674(79)90186-7. [DOI] [PubMed] [Google Scholar]
  30. Schindler J., Hynes R., Hopkins N. Evidence for recombination between N- and B-tropic murine leukemia viruses: analysis of three virion proteins by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. J Virol. 1977 Sep;23(3):700–700. doi: 10.1128/jvi.23.3.700-.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Shinnick T. M., Lerner R. A., Sutcliffe J. G. Nucleotide sequence of Moloney murine leukaemia virus. Nature. 1981 Oct 15;293(5833):543–548. doi: 10.1038/293543a0. [DOI] [PubMed] [Google Scholar]
  32. Sveda M. M., Soeiro R. Host restriction of Friend leukemia virus: synthesis and integration of the provirus. Proc Natl Acad Sci U S A. 1976 Jul;73(7):2356–2360. doi: 10.1073/pnas.73.7.2356. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Tennant R. W., Otten J. A., Brown A., Yang W. K., Kennel S. J. Characterization of Fv-1 host range strains of murine retroviruses by titration and p30 protein characteristics. Virology. 1979 Dec;99(2):349–357. doi: 10.1016/0042-6822(79)90014-x. [DOI] [PubMed] [Google Scholar]
  34. Van Beveren C., van Straaten F., Galleshaw J. A., Verma I. M. Nucleotide sequence of the genome of a murine sarcoma virus. Cell. 1981 Nov;27(1 Pt 2):97–108. doi: 10.1016/0092-8674(81)90364-0. [DOI] [PubMed] [Google Scholar]
  35. Yang W. K., Boone L. R., Tennant R. W., Brown A. Restriction of murine leukemia viruses by Fv-1: a model for studying host genetic control of retroviral gene movement and leukemogenesis. Prog Nucleic Acid Res Mol Biol. 1983;29:175–192. doi: 10.1016/s0079-6603(08)60446-8. [DOI] [PubMed] [Google Scholar]
  36. Yang W. K., Kiggans J. O., Yang D. M., Ou C. Y., Tennant R. W., Brown A., Bassin R. H. Synthesis and circularization of N- and B-tropic retroviral DNA Fv-1 permissive and restrictive mouse cells. Proc Natl Acad Sci U S A. 1980 May;77(5):2994–2998. doi: 10.1073/pnas.77.5.2994. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES