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. 1984 Jan;49(1):171–177. doi: 10.1128/jvi.49.1.171-177.1984

Amplification of a specific set of intracisternal A-particle genes in a mouse plasmacytoma.

G L Shen-Ong, M D Cole
PMCID: PMC255438  PMID: 6690709

Abstract

Intracisternal A-particles (IAPs) derive from endogenous retrovirus-like genes that are present in ca. 1,000 copies per haploid mouse genome. A particular IAP sequence variant that represents only 2% of the endogenous genes was found to be the predominant transcript in five out of seven plasmacytoma lines examined, and these genes have been amplified two- to fourfold in MOPC 315. Restriction enzyme digests of normal and tumor DNAs revealed a set of conserved sites within the population of amplified genes which could be used to generate a consensus restriction enzyme map of the amplified IAP genome. Molecular clones of IAP genes related to those amplified in MOPC 315 were isolated from gene libraries of MOPC 315 and MOPC 104E DNAs. Each clone was characterized for the presence of the restriction enzyme sites conserved in the amplified genes. Of 26 clones isolated from MOPC 315 DNA, 14 contained all of the conserved sites, whereas none of the clones isolated from MOPC 104E DNA contained all of the sites. These results suggested that approximately one-half of the genes isolated from MOPC 315 DNA were members of the amplified IAP gene population, representing newly acquired proviruses in the tumor DNA. Unique cellular flanking sequences were isolated from four of the MOPC 315 clones and four of the MOPC 104E clones. Probes from three of the MOPC 315 clones detected restriction fragments that were unique to MOPC 315 DNA and equivalent in size to the cloned gene, demonstrating that these three IAP genes had integrated into new sites in the plasmacytoma DNA. The other five clones were found to be endogenous to the BALB/c genome. Thus, IAPs represent a significant source of insertional mutations for any cell expressing the retrovirus-like particles. Furthermore, these results suggest that only one or a small number of endogenous IAP sequences are activated to give the homogeneous population of amplified genes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andersson J., Buxbaum J., Citronbaum R., Douglas S., Forni L., Melchers F., Pernis B., Stott D. IgM-producing tumors in the BALB-c mouse: a model for B-cell maturation. J Exp Med. 1974 Sep 1;140(3):742–763. doi: 10.1084/jem.140.3.742. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Benton W. D., Davis R. W. Screening lambdagt recombinant clones by hybridization to single plaques in situ. Science. 1977 Apr 8;196(4286):180–182. doi: 10.1126/science.322279. [DOI] [PubMed] [Google Scholar]
  3. Biczysko W., Pienkowski M., Solter D., Koprowski H. Virus particles in early mouse embryos. J Natl Cancer Inst. 1973 Sep;51(3):1041–1050. doi: 10.1093/jnci/51.3.1041. [DOI] [PubMed] [Google Scholar]
  4. Blattner F. R., Williams B. G., Blechl A. E., Denniston-Thompson K., Faber H. E., Furlong L., Grunwald D. J., Kiefer D. O., Moore D. D., Schumm J. W. Charon phages: safer derivatives of bacteriophage lambda for DNA cloning. Science. 1977 Apr 8;196(4286):161–169. doi: 10.1126/science.847462. [DOI] [PubMed] [Google Scholar]
  5. Calarco P. G., Szollosi D. Intracisternal A particles in ova and preimplantation stages of the mouse. Nat New Biol. 1973 May 16;243(124):91–93. [PubMed] [Google Scholar]
  6. Chase D. G., Pikó L. Expression of A- and C-type particles in early mouse embryos. J Natl Cancer Inst. 1973 Dec;51(6):1971–1975. doi: 10.1093/jnci/51.6.1971. [DOI] [PubMed] [Google Scholar]
  7. DALTON A. J., POTTER M., MERWIN R. M. Some ultrastructural characteristics of a series of primary and transplanted plasma-cell tumors of the mouse. J Natl Cancer Inst. 1961 May;26:1221–1267. [PubMed] [Google Scholar]
  8. DE HARVEN E., FRIEND C. Electron microscope study of a cell-free induced leukemia of the mouse: a preliminary report. J Biophys Biochem Cytol. 1958 Mar 25;4(2):151–156. doi: 10.1083/jcb.4.2.151. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Davis R. W., Thomas M., Cameron J., St John T. P., Scherer S., Padgett R. A. Rapid DNA isolations for enzymatic and hybridization analysis. Methods Enzymol. 1980;65(1):404–411. doi: 10.1016/s0076-6879(80)65051-4. [DOI] [PubMed] [Google Scholar]
  10. Derman E., Krauter K., Walling L., Weinberger C., Ray M., Darnell J. E., Jr Transcriptional control in the production of liver-specific mRNAs. Cell. 1981 Mar;23(3):731–739. doi: 10.1016/0092-8674(81)90436-0. [DOI] [PubMed] [Google Scholar]
  11. FRIEDLAENDER M., MOORE D. H. Occurrence of bodies within endoplasmic reticulum of Ehrlich ascites tumor cells. Proc Soc Exp Biol Med. 1956 Aug-Sep;92(4):828–831. doi: 10.3181/00379727-92-22627. [DOI] [PubMed] [Google Scholar]
  12. Hayward W. S., Neel B. G., Astrin S. M. Activation of a cellular onc gene by promoter insertion in ALV-induced lymphoid leukosis. Nature. 1981 Apr 9;290(5806):475–480. doi: 10.1038/290475a0. [DOI] [PubMed] [Google Scholar]
  13. Kuff E. L., Feenstra A., Lueders K., Rechavi G., Givol D., Canaani E. Homology between an endogenous viral LTR and sequences inserted in an activated cellular oncogene. Nature. 1983 Apr 7;302(5908):547–548. doi: 10.1038/302547a0. [DOI] [PubMed] [Google Scholar]
  14. Kuff E. L., Feenstra A., Lueders K., Smith L., Hawley R., Hozumi N., Shulman M. Intracisternal A-particle genes as movable elements in the mouse genome. Proc Natl Acad Sci U S A. 1983 Apr;80(7):1992–1996. doi: 10.1073/pnas.80.7.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Lueders K. K., Kuff E. L. Sequences associated with intracisternal A particles are reiterated in the mouse genome. Cell. 1977 Dec;12(4):963–972. doi: 10.1016/0092-8674(77)90161-1. [DOI] [PubMed] [Google Scholar]
  16. McIntimif K. R., Asofsky R. M., Potter M., Kuff E. L. Macroglobulin-Producing Plasma-Cell Tumor in Mice: Identification of a New Light Chain. Science. 1965 Oct 15;150(3694):361–363. doi: 10.1126/science.150.3694.361. [DOI] [PubMed] [Google Scholar]
  17. Ono M., Cole M. D., White A. T., Huang R. C. Sequence organization of cloned intracisternal A particle genes. Cell. 1980 Sep;21(2):465–473. doi: 10.1016/0092-8674(80)90483-3. [DOI] [PubMed] [Google Scholar]
  18. Paterson B. M., Segal S., Lueders K. K., Kuff E. L. RNA associated with murine intracisternal type A particles codes for the main particle protein. J Virol. 1978 Jul;27(1):118–126. doi: 10.1128/jvi.27.1.118-126.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Rave N., Crkvenjakov R., Boedtker H. Identification of procollagen mRNAs transferred to diazobenzyloxymethyl paper from formaldehyde agarose gels. Nucleic Acids Res. 1979 Aug 10;6(11):3559–3567. doi: 10.1093/nar/6.11.3559. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Shen-Ong G. L., Cole M. D. Differing populations of intracisternal A-particle genes in myeloma tumors and mouse subspecies. J Virol. 1982 May;42(2):411–421. doi: 10.1128/jvi.42.2.411-421.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Thomas P. S. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201–5205. doi: 10.1073/pnas.77.9.5201. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Wahl G. M., Stern M., Stark G. R. Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate. Proc Natl Acad Sci U S A. 1979 Aug;76(8):3683–3687. doi: 10.1073/pnas.76.8.3683. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Wivel N. A., Smith G. H. Distribution of intracisternal A-particles in a variety of normal and neoplastic mouse tissues. Int J Cancer. 1971 Jan 15;7(1):167–175. doi: 10.1002/ijc.2910070119. [DOI] [PubMed] [Google Scholar]

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