Abstract
Studies on trachoma have been carried out on Taiwan since 1958 by the US Naval Medical Research Unit No. 2. Routine methods for the collection of virus specimens, storage and processing of successful virus isolation are presented in this paper.
A special virus isolation study on clinically active cases has shown that 49% positive diagnoses were made at the first examination, 58% at the second and 63% at the third. Detection of virus positive specimens by inclusion bodies alone would have diagnosed only 19%, 30% and 34% of the cases. Combining results of both tests increased the positive diagnosis to 57%, 64% and 68% respectively. Administration of oxytetracycline ophthalmic ointment for two series of intermittent treatments reduced virus isolations by 48% in a single test. The clinical findings in the treated cases showed only minor changes.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BERNKOPF H., NISHMI M., MAYTHAR B., FEITELBERG I. Trachoma infection in a human volunteer produced by egg-cultured virus. AMA Arch Ophthalmol. 1959 Jul;62(1):33–34. doi: 10.1001/archopht.1959.04220010037004. [DOI] [PubMed] [Google Scholar]
- COLLIER L. H., SOWA J. Isolation of trachoma virus in embryonate eggs. Lancet. 1958 May 10;1(7028):993–996. doi: 10.1016/s0140-6736(58)91800-2. [DOI] [PubMed] [Google Scholar]
- GRAYSTON J. T., WANG S. P., JOHNSTON P. B. Etiologic studies of trachoma on Taiwan. Proc Soc Exp Biol Med. 1960 Mar;103:596–599. doi: 10.3181/00379727-103-25607. [DOI] [PubMed] [Google Scholar]
- GRAYSTON J. T., WANG S. P., WOOLRIDGE R. L., YANG Y. F., JOHNSTON P. B. Trachoma-studies of etiology, laboratory diagnosis, and prevention. J Am Med Assoc. 1960 Apr 9;172:1577–1586. doi: 10.1001/jama.1960.03020150001001. [DOI] [PubMed] [Google Scholar]
- HANNA L., THYGESON P., JAWETZ E. Elementary-body virus isolated from clinical trachoma in California. Science. 1959 Nov 13;130(3385):1339–1340. doi: 10.1126/science.130.3385.1339. [DOI] [PubMed] [Google Scholar]
- MANN I., GREER C. H., PERRET D., MCLEAN C. Experimental trachoma produced by a West Australian virus. Br J Ophthalmol. 1960 Nov;44:641–648. doi: 10.1136/bjo.44.11.641. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SNYDER J. C., PAGE R. C., MURRAY E. S., DAGGY R. H., BELL S. D., Jr, NICHOLS R. L., HADDAD N. A., HANNA A. T., McCOMB D. Observations on the etiology of trachoma. Am J Ophthalmol. 1959 Sep;48(3):325–329. doi: 10.1016/0002-9394(59)90275-2. [DOI] [PubMed] [Google Scholar]
- SOWA J., COLLIER L. H. Isolation of trachoma virus from patients in West Africa. J Hyg (Lond) 1960 Mar;58:99–108. doi: 10.1017/s0022172400038158. [DOI] [PMC free article] [PubMed] [Google Scholar]
- TANG F. F., CHANG H. L., HUANG Y. T., WANG K. C. Studies on the etiology of trachoma with special reference to isolation of the virus in chick embryo. Chin Med J. 1957 Jun;75(6):429–447. [PubMed] [Google Scholar]