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. 1984 May;50(2):316–324. doi: 10.1128/jvi.50.2.316-324.1984

Replication of simian herpesvirus SA8 and identification of viral polypeptides in infected cells.

R Eberle, J K Hilliard
PMCID: PMC255623  PMID: 6708170

Abstract

The replication of the simian herpesvirus SA8 in Vero cells was examined. The time course of replication of the simian herpesvirus SA8 was found to be similar to that of the herpes simplex viruses. Infectious progeny virions were first detectable by 6 h postinfection and were readily released into the extracellular fluids beginning at 9 h postinfection. All cell lines tested, with the exception of Madin-Darby canine kidney cells, were permissive for SA8. Analysis of SA8-infected cells by sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed over 40 infected cell polypeptides ranging in molecular weight from 158,000 to less than 10,000. Of these proteins, 23 were present in virions. Three classes of infected cell polypeptides could be identified based on the kinetics of their synthesis. Post-translational processing of several SA8-induced proteins was also observed in pulse-chase experiments. Six distinct SA8-specific glycoproteins ranging from 118,000 to 19,500 daltons were also identified in infected cells. Of these glycoproteins, five were present in virions.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Balachandran N., Harnish D., Rawls W. E., Bacchetti S. Glycoproteins of herpes simplex virus type 2 as defined by monoclonal antibodies. J Virol. 1982 Oct;44(1):344–355. doi: 10.1128/jvi.44.1.344-355.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Barahona H., Melendez L. V., Melnick J. L. A compendium of herpesviruses isolated from non-human primates. Intervirology. 1974;3(3):175–192. doi: 10.1159/000149754. [DOI] [PubMed] [Google Scholar]
  3. Bittlingmaier K., Schneider D., Falke D. Uptake of [3H] thymidine and cell DNA synthesis during the early multiplication phase of herpesvirus hominis in BHK cells. Biochim Biophys Acta. 1975 Nov 4;407(4):384–391. doi: 10.1016/0005-2787(75)90290-7. [DOI] [PubMed] [Google Scholar]
  4. Bookout J. B., Levy C. C. Comparative examination of the polypeptides of herpes simplex virus: types 1 and 2. Virology. 1980 Feb;101(1):198–216. doi: 10.1016/0042-6822(80)90496-1. [DOI] [PubMed] [Google Scholar]
  5. Boulter E. A. The isolation of monkey B virus (Herpesvirus simiae) from the trigeminal ganglia of a healthy seropositive rhesus monkey. J Biol Stand. 1975;3(3):279–280. doi: 10.1016/0092-1157(75)90031-1. [DOI] [PubMed] [Google Scholar]
  6. Bryan B. L., Espana C. D., Emmons R. W., Vijayan N., Hoeprich P. D. Recovery from encephalomyelitis caused by Herpesvirus simiae. Report of a case. Arch Intern Med. 1975 Jun;135(6):868–870. [PubMed] [Google Scholar]
  7. DAVIDSON W. L., HUMMELER K. B virus infection in man. Ann N Y Acad Sci. 1960 May 12;85:970–979. doi: 10.1111/j.1749-6632.1960.tb50017.x. [DOI] [PubMed] [Google Scholar]
  8. Eberle R., Courtney R. J. Preparation and characterization of specific antisera to individual glycoprotein antigens comprising the major glycoprotein region of herpes simplex virus type 1. J Virol. 1980 Sep;35(3):902–917. doi: 10.1128/jvi.35.3.902-917.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Eberle R., Courtney R. J. gA and gB glycoproteins of herpes simplex virus type 1: two forms of a single polypeptide. J Virol. 1980 Dec;36(3):665–675. doi: 10.1128/jvi.36.3.665-675.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Gibson W., Roizman B. Proteins specified by herpes simplex virus. Staining and radiolabeling properties of B capsid and virion proteins in polyacrylamide gels. J Virol. 1974 Jan;13(1):155–165. doi: 10.1128/jvi.13.1.155-165.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hampar B., Stevens D. A., Martos L. M., Ablashi D. V., Burroughs M. A., Wells G. A. Correlation between the neutralizing activity of human serum against Herpes simplex virus and a simian herpesvirus (SA8). J Immunol. 1969 Feb;102(2):397–403. [PubMed] [Google Scholar]
  12. Heilman C. J., Jr, Zweig M., Stephenson J. R., Hampar B. Isolation of a nucleocapsid polypeptide of herpes simplex virus types 1 and 2 possessing immunologically type-specific and cross-reactive determinants. J Virol. 1979 Jan;29(1):34–42. doi: 10.1128/jvi.29.1.34-42.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Honess R. W., Roizman B. Proteins specified by herpes simplex virus. XI. Identification and relative molar rates of synthesis of structural and nonstructural herpes virus polypeptides in the infected cell. J Virol. 1973 Dec;12(6):1347–1365. doi: 10.1128/jvi.12.6.1347-1365.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. KEEBLE S. A. B virus infection in monkeys. Ann N Y Acad Sci. 1960 May 12;85:960–969. doi: 10.1111/j.1749-6632.1960.tb50016.x. [DOI] [PubMed] [Google Scholar]
  15. Kalter S. S., Ablashi D., Espana C., Heberling R. L., Hull R. N., Lennette E. H., Malherbe H. H., McConnell S., Yohn D. S. Simian virus nomenclature, 1980. Intervirology. 1980;13(6):317–330. doi: 10.1159/000149142. [DOI] [PubMed] [Google Scholar]
  16. Kalter S. S., Heberling R. L. Comparative virology of primates. Bacteriol Rev. 1971 Sep;35(3):310–364. doi: 10.1128/br.35.3.310-364.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Kalter S. S., Weiss S. A., Heberling R. L., Guajardo J. E., Smith G. C., 3rd The isolation of herpesvirus from trigeminal ganglia of normal baboons (Papio cynocephalus). Lab Anim Sci. 1978 Dec;28(6):705–709. [PubMed] [Google Scholar]
  18. Killington R. A., Yeo J., Honess R., Watson D. H., Duncan B. E., Halliburton I. W., Mumford J. Comparative analyses of the proteins and antigens of five herpesviruses. J Gen Virol. 1977 Nov;37(2):297–310. doi: 10.1099/0022-1317-37-2-297. [DOI] [PubMed] [Google Scholar]
  19. MALHERBE H., HARWIN R. Seven viruses isolated from the vervet monkey. Br J Exp Pathol. 1957 Oct;38(5):539–541. [PMC free article] [PubMed] [Google Scholar]
  20. MALHERBE H., HARWIN R. The cytopathic effects of vervet monkey viruses. S Afr Med J. 1963 Apr 20;37:407–411. [PubMed] [Google Scholar]
  21. MELNICK J. L., BANKER D. D. Isolation of B virus (herpes group) from the central nervous system of a rhesus monkey. J Exp Med. 1954 Aug 1;100(2):181–194. doi: 10.1084/jem.100.2.181. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Malherbe H., Strickland-Cholmley M. Simian herpesvirus SA8 from a baboon. Lancet. 1969 Dec 27;2(7635):1427–1427. doi: 10.1016/s0140-6736(69)90972-6. [DOI] [PubMed] [Google Scholar]
  23. McCarthy K., Tosolini F. A. A review of primate herpes viruses. Proc R Soc Med. 1975 Mar;68(3):145–150. [PMC free article] [PubMed] [Google Scholar]
  24. Misra V., Blumenthal R. M., Babiuk L. A. Proteins Specified by bovine herpesvirus 1 (infectious bovine rhinotracheitis virus). J Virol. 1981 Nov;40(2):367–378. doi: 10.1128/jvi.40.2.367-378.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Perdue M. L., Kemp M. C., Randall C. C., O'Callaghan D. J. Studies of the molecular anatomy of the L-M cell strain of equine herpes virus type 1: proteins of the nucleocapsid and intact virion. Virology. 1974 May;59(1):201–216. doi: 10.1016/0042-6822(74)90216-5. [DOI] [PubMed] [Google Scholar]
  26. Pereira L., Cassai E., Honess R. W., Roizman B., Terni M., Nahmias A. Variability in the structural polypeptides of herpes simplex virus 1 strains: potential application in molecular epidemiology. Infect Immun. 1976 Jan;13(1):211–220. doi: 10.1128/iai.13.1.211-220.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Pereira L., Dondero D., Norrild B., Roizman B. Differential immunologic reactivity and processing of glycoproteins gA and gB of herpes simplex virus types 1 and 2 made in Vero and HEp-2 cells. Proc Natl Acad Sci U S A. 1981 Aug;78(8):5202–5206. doi: 10.1073/pnas.78.8.5202. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Powell K. L., Courtney R. J. Polypeptide synthesized in herpes simplex virus type 2-infected HEp-2 cells. Virology. 1975 Jul;66(1):217–228. doi: 10.1016/0042-6822(75)90192-0. [DOI] [PubMed] [Google Scholar]
  29. Randall R. E., Honess R. W., O'Hare P. Proteins specified by herpesvirus saimiri: identification and properties of virus-specific polypeptides in productively infected cells. J Gen Virol. 1983 Jan;64(Pt 1):19–35. doi: 10.1099/0022-1317-64-1-19. [DOI] [PubMed] [Google Scholar]
  30. Spear P. G. Membrane proteins specified by herpes simplex viruses. I. Identification of four glycoprotein precursors and their products in type 1-infected cells. J Virol. 1976 Mar;17(3):991–1008. doi: 10.1128/jvi.17.3.991-1008.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Stevely W. S. Virus-induced proteins in pseudorabies-infected cells. II. Proteins of the virion and nucleocapsid. J Virol. 1975 Oct;16(4):944–950. doi: 10.1128/jvi.16.4.944-950.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Stevens D. A., Pincus T., Burroughs M. A., Hampar B. Serologic relationship o a simian Herpes virus (SA8) and Herpes simplex virus: heterogeneity in the degree of reciprocal cross-reactivity shown by rabbit 7S and 19S antibodies. J Immunol. 1968 Nov;101(5):979–983. [PubMed] [Google Scholar]

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