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. 1987 Aug;61(8):2428–2437. doi: 10.1128/jvi.61.8.2428-2437.1987

The 65,000-Mr DNA-binding and virion trans-inducing proteins of herpes simplex virus type 1.

H S Marsden, M E Campbell, L Haarr, M C Frame, D S Parris, M Murphy, R G Hope, M T Muller, C M Preston
PMCID: PMC255661  PMID: 3037105

Abstract

The possible identity of the herpes simplex virus type 1 (HSV-1) 65K (65,000-Mr) virion protein which stimulates transcription from immediate-early genes with the HSV-1 65K DNA-binding protein was investigated. The two proteins were found to be distinct by the three separate criteria of immunological reactivity, tryptic peptide fingerprinting, and mobility in two-dimensional gels. Using HSV-1/HSV-2 intertypic recombinants and a serotype-specific antiserum, we located the gene encoding the 65K DNA-binding protein between coordinates 0.574 and 0.682 on the HSV-1 genome. The protein is posttranslationally modified by phosphorylation. In crude extracts of HSV-1-infected cells the 65K trans-inducing protein did not detectably bind to double-stranded calf thymus DNA under the conditions of our assay.

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