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. 1987 Aug;61(8):2627–2630. doi: 10.1128/jvi.61.8.2627-2630.1987

Characterization of capsid and noncapsid proteins of B19 parvovirus propagated in human erythroid bone marrow cell cultures.

K Ozawa, N Young
PMCID: PMC255713  PMID: 3599184

Abstract

The major capsid and noncapsid proteins of the pathogenic parvovirus B19, propagated in vitro, were detected by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, immunoprecipitation, and immunoblot of the erythroid fraction of infected human bone marrow cell cultures. There were two capsid proteins of 58 kilodaltons (kDa; the major species) and 84 kDa (the minor species). Newly synthesized capsid viral proteins were present in the supernatants of infected cultures. The major noncapsid protein of 77 kDa was localized to the nucleus.

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Selected References

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  1. Anderson M. J., Higgins P. G., Davis L. R., Willman J. S., Jones S. E., Kidd I. M., Pattison J. R., Tyrrell D. A. Experimental parvoviral infection in humans. J Infect Dis. 1985 Aug;152(2):257–265. doi: 10.1093/infdis/152.2.257. [DOI] [PubMed] [Google Scholar]
  2. Barbacid M., Lauver A. V., Devare S. G. Biochemical and immunological characterization of polyproteins coded for by the McDonough, Gardner-Arnstein, and Snyder-Theilen strains of feline sarcoma virus. J Virol. 1980 Jan;33(1):196–207. doi: 10.1128/jvi.33.1.196-207.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bloom M. E., Race R. E., Wolfinbarger J. B. Identification of a nonvirion protein of Aleutian disease virus: mink with Aleutian disease have antibody to both virion and nonvirion proteins. J Virol. 1982 Aug;43(2):608–616. doi: 10.1128/jvi.43.2.608-616.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cotmore S. F., McKie V. C., Anderson L. J., Astell C. R., Tattersall P. Identification of the major structural and nonstructural proteins encoded by human parvovirus B19 and mapping of their genes by procaryotic expression of isolated genomic fragments. J Virol. 1986 Nov;60(2):548–557. doi: 10.1128/jvi.60.2.548-557.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cotmore S. F., Sturzenbecker L. J., Tattersall P. The autonomous parvovirus MVM encodes two nonstructural proteins in addition to its capsid polypeptides. Virology. 1983 Sep;129(2):333–343. doi: 10.1016/0042-6822(83)90172-1. [DOI] [PubMed] [Google Scholar]
  6. Cotmore S. F., Tattersall P. Organization of nonstructural genes of the autonomous parvovirus minute virus of mice. J Virol. 1986 Jun;58(3):724–732. doi: 10.1128/jvi.58.3.724-732.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Janik J. E., Huston M. M., Rose J. A. Adeno-associated virus proteins: origin of the capsid components. J Virol. 1984 Nov;52(2):591–597. doi: 10.1128/jvi.52.2.591-597.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  9. Lederman M., Chen K. C., Stout E. R., Bates R. C. Possible sequences for nuclear accumulation of parvoviral proteins. Cell Biol Int Rep. 1986 May;10(5):383–387. doi: 10.1016/0309-1651(86)90010-x. [DOI] [PubMed] [Google Scholar]
  10. Matsunaga Y., Matsuno S. Structural and nonstructural proteins of a rabbit parvovirus. J Virol. 1983 Feb;45(2):627–633. doi: 10.1128/jvi.45.2.627-633.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Mendelson E., Trempe J. P., Carter B. J. Identification of the trans-acting Rep proteins of adeno-associated virus by antibodies to a synthetic oligopeptide. J Virol. 1986 Dec;60(3):823–832. doi: 10.1128/jvi.60.3.823-832.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Ozawa K., Ayub J., Hao Y. S., Kurtzman G., Shimada T., Young N. Novel transcription map for the B19 (human) pathogenic parvovirus. J Virol. 1987 Aug;61(8):2395–2406. doi: 10.1128/jvi.61.8.2395-2406.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Ozawa K., Kurtzman G., Young N. Replication of the B19 parvovirus in human bone marrow cell cultures. Science. 1986 Aug 22;233(4766):883–886. doi: 10.1126/science.3738514. [DOI] [PubMed] [Google Scholar]
  14. Paradiso P. R. Identification of multiple forms of the noncapsid parvovirus protein NCVP1 in H-1 parvovirus-infected cells. J Virol. 1984 Oct;52(1):82–87. doi: 10.1128/jvi.52.1.82-87.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Porter D. D., Porter H. G., Larsen A. E., Hadlow W. J. Immunoenzyme Western blotting analysis of antibody specificity in Aleutian disease of mink, a parvovirus infection. J Virol. 1984 Dec;52(3):745–749. doi: 10.1128/jvi.52.3.745-749.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Shade R. O., Blundell M. C., Cotmore S. F., Tattersall P., Astell C. R. Nucleotide sequence and genome organization of human parvovirus B19 isolated from the serum of a child during aplastic crisis. J Virol. 1986 Jun;58(3):921–936. doi: 10.1128/jvi.58.3.921-936.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Tattersall P., Cawte P. J., Shatkin A. J., Ward D. C. Three structural polypeptides coded for by minite virus of mice, a parvovirus. J Virol. 1976 Oct;20(1):273–289. doi: 10.1128/jvi.20.1.273-289.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Young N., Mortimer P. Viruses and bone marrow failure. Blood. 1984 Apr;63(4):729–737. [PubMed] [Google Scholar]

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