Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1987 Sep;61(9):2764–2769. doi: 10.1128/jvi.61.9.2764-2769.1987

Role of pseudorabies virus glycoprotein gI in virus release from infected cells.

T C Mettenleiter, C Schreurs, F Zuckermann, T Ben-Porat
PMCID: PMC255784  PMID: 3039168

Abstract

The Bartha vaccine strain of pseudorabies virus has a deletion in the short unique (Us) region of its genome which includes the genes that code for glycoproteins gI and gp63 (E. Petrovskis, J. G. Timmins, T. M. Gierman, and L. E. Post, J. Virol. 60:1166-1169, 1986). Restoration of an intact Us to the Bartha strain enhances its ability to be released from infected rabbit kidney cells and increases the size of the plaques formed on these cells (T. Ben-Porat, J. M. DeMarchi, J. Pendrys, R. A. Veach, and A. S. Kaplan, J. Virol. 57:191-196, 1986). To determine which gene function plays a role in virus release from rabbit kidney cells, deletions were introduced into the genomes of both wild-type virus and the "rescued" Bartha strain (Bartha strain to which an intact Us had been restored) that abolish the expression of either the gI gene alone or both gI and gp63 genes. The effect of these deletions on the phenotype of the viruses was studied. Deletion mutants of wild-type virus defective in either gI or gI and gp63 behave like wild-type virus with respect to virus release and plaque size on rabbit kidney cells. Deletion of gI from the rescued Bartha strain, however, strongly affects virus release and causes a decrease in plaque size. We conclude that gI affects virus release but that at least one other viral function also affects this process. This function is defective in the Bartha strain but not in wild-type virus; in its absence gI is essential to efficient release of the virus from rabbit kidney cells.

Full text

PDF
2765

Images in this article

2765Image
on p.2765
2766Image
on p.2766
2766Image
on p.2766
2766Image
on p.2766
2768Image
on p.2768

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ben-Porat T., DeMarchi J., Pendrys J., Veach R. A., Kaplan A. S. Proteins specified by the short unique region of the genome of pseudorabies virus play a role in the release of virions from certain cells. J Virol. 1986 Jan;57(1):191–196. doi: 10.1128/jvi.57.1.191-196.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Ben-Porat T., Kaplan A. S. A comparison of two populations of defective, interfering pseudorabies virus particles. Virology. 1976 Jul 15;72(2):471–479. doi: 10.1016/0042-6822(76)90175-6. [DOI] [PubMed] [Google Scholar]
  3. Berns A., van den Ouweland A., Quint W., van Oirschot J., Gielkens A. Presence of markers for virulence in the unique short region or repeat region or both of pseudorabies hybrid viruses. J Virol. 1985 Jan;53(1):89–93. doi: 10.1128/jvi.53.1.89-93.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. EAGLE H. Amino acid metabolism in mammalian cell cultures. Science. 1959 Aug 21;130(3373):432–437. doi: 10.1126/science.130.3373.432. [DOI] [PubMed] [Google Scholar]
  5. Gielkens A. L., Van Oirschot J. T., Berns A. J. Genome differences among field isolates and vaccine strains of pseudorabies virus. J Gen Virol. 1985 Jan;66(Pt 1):69–82. doi: 10.1099/0022-1317-66-1-69. [DOI] [PubMed] [Google Scholar]
  6. Hampl H., Ben-Porat T., Ehrlicher L., Habermehl K. O., Kaplan A. S. Characterization of the envelope proteins of pseudorabies virus. J Virol. 1984 Nov;52(2):583–590. doi: 10.1128/jvi.52.2.583-590.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. KAPLAN A. S., BEN-PORAT T. The action of 5-fluorouracil on the nucleic acid metabolism of pseudorabies virus-infected and noninfected rabbit kidney cells. Virology. 1961 Jan;13:78–92. doi: 10.1016/0042-6822(61)90034-4. [DOI] [PubMed] [Google Scholar]
  8. KAPLAN A. S., VATTER A. E. A comparison of herpes simplex and pseudorabies viruses. Virology. 1959 Apr;7(4):394–407. doi: 10.1016/0042-6822(59)90068-6. [DOI] [PubMed] [Google Scholar]
  9. Kessler S. W. Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A. J Immunol. 1975 Dec;115(6):1617–1624. [PubMed] [Google Scholar]
  10. Lomniczi B., Blankenship M. L., Ben-Porat T. Deletions in the genomes of pseudorabies virus vaccine strains and existence of four isomers of the genomes. J Virol. 1984 Mar;49(3):970–979. doi: 10.1128/jvi.49.3.970-979.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lomniczi B., Watanabe S., Ben-Porat T., Kaplan A. S. Genetic basis of the neurovirulence of pseudorabies virus. J Virol. 1984 Oct;52(1):198–205. doi: 10.1128/jvi.52.1.198-205.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lomniczi B., Watanabe S., Ben-Porat T., Kaplan A. S. Genome location and identification of functions defective in the Bartha vaccine strain of pseudorabies virus. J Virol. 1987 Mar;61(3):796–801. doi: 10.1128/jvi.61.3.796-801.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Mettenleiter T. C., Lukacs N., Rziha H. J. Mapping of the structural gene of pseudorabies virus glycoprotein A and identification of two non-glycosylated precursor polypeptides. J Virol. 1985 Jan;53(1):52–57. doi: 10.1128/jvi.53.1.52-57.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Mettenleiter T. C., Lukàcs N., Rziha H. J. Pseudorabies virus avirulent strains fail to express a major glycoprotein. J Virol. 1985 Oct;56(1):307–311. doi: 10.1128/jvi.56.1.307-311.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Mettenleiter T. C., Schreurs C., Thiel H. J., Rziha H. J. Variability of pseudorabies virus glycoprotein I expression. Virology. 1987 May;158(1):141–146. doi: 10.1016/0042-6822(87)90247-9. [DOI] [PubMed] [Google Scholar]
  16. Petrovskis E. A., Timmins J. G., Gierman T. M., Post L. E. Deletions in vaccine strains of pseudorabies virus and their effect on synthesis of glycoprotein gp63. J Virol. 1986 Dec;60(3):1166–1169. doi: 10.1128/jvi.60.3.1166-1169.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Petrovskis E. A., Timmins J. G., Post L. E. Use of lambda gt11 to isolate genes for two pseudorabies virus glycoproteins with homology to herpes simplex virus and varicella-zoster virus glycoproteins. J Virol. 1986 Oct;60(1):185–193. doi: 10.1128/jvi.60.1.185-193.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Platt K. B., Maré C. J., Hinz P. N. Differentiation of vaccine strains and field isolates of pseudorabies (Aujeszky's disease) virus: trypsin sensitivity and mouse virulence markers. Arch Virol. 1980;63(2):107–114. doi: 10.1007/BF01320767. [DOI] [PubMed] [Google Scholar]
  19. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  20. Villarreal L. P., Berg P. Hybridization in situ of SV40 plaques: detection of recombinant SV40 virus carrying specific sequences of nonviral DNA. Science. 1977 Apr 8;196(4286):183–185. doi: 10.1126/science.191907. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES