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. 1982 Apr;42(1):292–296. doi: 10.1128/jvi.42.1.292-296.1982

New way to isolate simian virus 40 nucleoprotein complexes from infected cells: use of a thiol-specific reagent.

F M Boyce, O Sundin, J Barsoum, A Varshavsky
PMCID: PMC256070  PMID: 6283146

Abstract

A new method for the isolation of simian virus 40 nucleoprotein complexes from nuclei of lytically infected cells is described. The method is based on the addition of a thiol-specific reagent, 5'5'-dithiobis(2-nitrobenzoic acid), to lysis and extraction buffers. By inhibiting an uncoating activity during simian virus 40 extraction, 5'5'-dithiobis (2-nitrobenzoic acid) allows the use of efficient extraction buffers, such as one containing Triton X-100 and EDTA, for the isolation of native simian virus 40 minichromosomes and virion-type structures. Use of the method is illustrated by following encapsidation of simian virus 40 minichromosomes in a pulse-chase experiment. Since 5'5'-dithiobis (2-nitrobenzoic acid) is an inhibitor of many different enzymes, the 5',5'-dithiobis (2-nitrobenzoic acid) extraction technique may be useful for the isolation of not only papovaviruses but also other viruses and possibly cellular chromatin.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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