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. 1982 Apr;42(1):301–305. doi: 10.1128/jvi.42.1.301-305.1982

Mechanism of replication of bacteriophage phi X174 XIX. Initiation of phi X174 viral strand DNA synthesis at internal sites on the genome.

M Matthes, P J Weisbeek, D T Denhardt
PMCID: PMC256072  PMID: 6211552

Abstract

Bacteriophage phi X174 viral strand DNA molecules shorter than genome length found late in the infectious cycle in Escherichia coli were 5' end labeled with 32P. Hybridization of the 32P-labeled molecules to restriction enzyme fragments of phi X replicative form DNA revealed an excess of phi X molecules whose 5' ends mapped in HaeIII fragments Z3 and Z4 in comparison with fragments Z1 and Z2. This suggests that initiation of phi X174 viral strand DNA synthesis may occur at internal sites on the complementary strand. There are several appropriately located sequences that might serve as n' (factor Y) recognition sequences and thereby facilitate discontinuous synthesis of the viral strand.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arai K., Kornberg A. A general priming system employing only dnaB protein and primase for DNA replication. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4308–4312. doi: 10.1073/pnas.76.9.4308. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Arai K., Kornberg A. Unique primed start of phage phi X174 DNA replication and mobility of the primosome in a direction opposite chain synthesis. Proc Natl Acad Sci U S A. 1981 Jan;78(1):69–73. doi: 10.1073/pnas.78.1.69. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Dubeau L., Denhardt D. T. The mechanism of replication of phi X 174. XVIII. Gene A and A* proteins of phi X 174 bind tightly to phi X 174 replicative form DNA. Biochim Biophys Acta. 1981 Mar 26;653(1):52–60. doi: 10.1016/0005-2787(81)90103-9. [DOI] [PubMed] [Google Scholar]
  4. Eisenberg S. Cleavage of phi X174 single-stranded DNA by gene A protein and formation of a tight protein-DNA complex. J Virol. 1980 Aug;35(2):409–413. doi: 10.1128/jvi.35.2.409-413.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Eisenberg S., Finer M. Cleavage and circularization of single-stranded DNA: a novel enzymatic activity of phi X174 A* protein. Nucleic Acids Res. 1980 Nov 25;8(22):5305–5315. doi: 10.1093/nar/8.22.5305. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Eisenberg S., Kornberg A. Purification and characterization of phiX174 gene A protein. A multifunctional enzyme of duplex DNA replication. J Biol Chem. 1979 Jun 25;254(12):5328–5332. [PubMed] [Google Scholar]
  7. Gilbert W., Dressler D. DNA replication: the rolling circle model. Cold Spring Harb Symp Quant Biol. 1968;33:473–484. doi: 10.1101/sqb.1968.033.01.055. [DOI] [PubMed] [Google Scholar]
  8. Ikeda J. E., Yudelevich A., Shimamoto N., Hurwitz J. Role of polymeric forms of the bacteriophage phi X174 coded gene A protein in phi XRFI DNA cleavage. J Biol Chem. 1979 Oct 10;254(19):9416–9428. [PubMed] [Google Scholar]
  9. Langeveld S. A., van Mansfeld A. D., de Winter J. M., Weisbeek P. J. Cleavage of single-stranded DNA by the A and A* proteins of bacteriophage phi X174. Nucleic Acids Res. 1979 Dec 20;7(8):2177–2188. doi: 10.1093/nar/7.8.2177. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Langeveld S. A., van Mansfeld A. D., van der Ende A., van de Pol J. H., van Arkel G. A., Weisbeek P. J. The nuclease specificity of the bacteriophage phi X174 A* protein. Nucleic Acids Res. 1981 Feb 11;9(3):545–562. doi: 10.1093/nar/9.3.545. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Machida Y., Okazaki T., Okazaki R. Discontinuous replication of replicative form DNA from bacteriophage phiX174. Proc Natl Acad Sci U S A. 1977 Jul;74(7):2776–2779. doi: 10.1073/pnas.74.7.2776. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Matthes M., Denhardt D. T. Mechanism of replication of bacteriophage phi X174 XX. Sensitivity of nascent DNA to single-strand-specific nucleases. J Virol. 1982 Apr;42(1):12–19. doi: 10.1128/jvi.42.1.12-19.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Matthes M., Denhardt D. T. The mechanism of replication of phi x174 DNA. XVI. Evidence that the phi x174 viral strand is synthesized discontinuously. J Mol Biol. 1980 Jan 5;136(1):45–63. doi: 10.1016/0022-2836(80)90365-4. [DOI] [PubMed] [Google Scholar]
  14. McFadden G., Denhardt D. T. Mechanism of replication of phi x174 single-stranded DNA. IX. Requirement for the Escherichia coli dnaG protein. J Virol. 1974 Nov;14(5):1070–1075. doi: 10.1128/jvi.14.5.1070-1075.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. McFadden G., Denhardt D. T. The mechanism of replication of phiX174 DNA. XIII. Discontinuous synthesis of the complementary strand in an Escherichia coli host with a temperature-sensitive polynucleotide ligase. J Mol Biol. 1975 Nov 25;99(1):125–142. doi: 10.1016/s0022-2836(75)80163-x. [DOI] [PubMed] [Google Scholar]
  16. Miyamoto C., Denhardt D. T. Evidence for the presence of ribonucleotides at the 5' termini of some DNA molecules isolated from Escherichia coli polAex2. J Mol Biol. 1977 Nov;116(4):681–707. doi: 10.1016/0022-2836(77)90266-2. [DOI] [PubMed] [Google Scholar]
  17. Scott J. F., Kornberg A. Purification of the rep protein of Escherichia coli. An ATPase which separates duplex DNA strands in advance of replication. J Biol Chem. 1978 May 10;253(9):3292–3297. [PubMed] [Google Scholar]
  18. Shlomai J., Kornberg A. An Escherichia coli replication protein that recognizes a unique sequence within a hairpin region in phi X174 DNA. Proc Natl Acad Sci U S A. 1980 Feb;77(2):799–803. doi: 10.1073/pnas.77.2.799. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Sinsheimer R. L. Bacteriophage phi-X174 and related viruses. Prog Nucleic Acid Res Mol Biol. 1968;8:115–169. [PubMed] [Google Scholar]
  20. Sumida-Yasumoto C., Ikeda J. E., Benz E., Marians K. J., Vicuna R., Sugrue S., Zipursky S. L., Hurwitz J. Replication of phiX174 DNA: in vitro synthesis of phiX RFI DNA and circular, single-stranded DNA. Cold Spring Harb Symp Quant Biol. 1979;43(Pt 1):311–329. doi: 10.1101/sqb.1979.043.01.038. [DOI] [PubMed] [Google Scholar]
  21. Takahashi S., Hours C., Chu A., Denhardt D. T. The rep mutation. VI. Purification and properties of the Escherichia coli rep protein, DNA helicase III. Can J Biochem. 1979 Jun;57(6):855–866. doi: 10.1139/o79-105. [DOI] [PubMed] [Google Scholar]
  22. Wickner S., Hurwitz J. Association of phiX174 DNA-dependent ATPase activity with an Escherichia coli protein, replication factor Y, required for in vitro synthesis of phiX174 DNA. Proc Natl Acad Sci U S A. 1975 Sep;72(9):3342–3346. doi: 10.1073/pnas.72.9.3342. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Zipursky S. L., Marians K. J. Identification of two Escherichia coli factor Y effector sites near the origins of replication of the plasmids (ColE1 and pBR322. Proc Natl Acad Sci U S A. 1980 Nov;77(11):6521–6525. doi: 10.1073/pnas.77.11.6521. [DOI] [PMC free article] [PubMed] [Google Scholar]

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