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. 1982 Oct;44(1):256–262. doi: 10.1128/jvi.44.1.256-262.1982

Genetic specificity of DNA synthesized in the absence of T4 bacteriophage gene 44 protein.

A W Kozinski, S K Ling
PMCID: PMC256260  PMID: 6982976

Abstract

Upon infection of Escherichia coli B with T4 phage with DO amber mutation in gene 44, a minimal amount of phage DNA is synthesized. This progeny DNA is, for the most part, covalently attached to the parental DNA. Analysis of the genetic representation of this DNA was performed by hybridization to cloned genetic segments. It was shown that areas preferentially replicated differ from origins observed in "normal" replication: under normal conditions, there is a strong origin in the genetic area of genes 50-5 and lack of initiation within the group of genes 40-43 and 35-52. In contrast, in the absence of the gene 44 protein, the genetic area of 50-5 is underrepresented, genes 35-36, tRNA, and genes 40-41 are the most prominent among progeny DNA, and the area of gene 39 is least represented. Since the area of gene 35 is known from the genetic data or other to be a high-frequency recombination area, and since the area of gene 39 is known to display a low frequency of recombination, we postulate that the observed uptake of label occurs at the site-specific recombinational intersections.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Delius H., Howe C., Kozinski A. W. Structure of the replicating DNA from bacteriophage T4. Proc Natl Acad Sci U S A. 1971 Dec;68(12):3049–3053. doi: 10.1073/pnas.68.12.3049. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Halpern M. E., Mattson T., Kozinski A. W. Origins of phage T4 DNA replication as revealed by hybridization to cloned genes. Proc Natl Acad Sci U S A. 1979 Dec;76(12):6137–6141. doi: 10.1073/pnas.76.12.6137. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Halpern M., Mattson T., Kozinski A. W. Late events in T4 bacteriophage DNA replication. III. Specificity of DNA reinitiation as revealed by hybridization to cloned genetic fragments. J Virol. 1982 May;42(2):422–431. doi: 10.1128/jvi.42.2.422-431.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Howe C. C., Buckley P. J., Carlson K. M., Kozinski A. W. Multiple and specific initiation of T4 DNA replication. J Virol. 1973 Jul;12(1):130–148. doi: 10.1128/jvi.12.1.130-148.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. KOZINSKI A. W., SZYBALSKI W. Dispersive transfer of the parental DNA molecule to the progeny of phage phiX-174. Virology. 1959 Oct;9:260–274. doi: 10.1016/0042-6822(59)90119-9. [DOI] [PubMed] [Google Scholar]
  6. Kozinski A. W., Doermann A. H., Kozinski P. B. Absence of interparental recombination in multiplicity reconstitution from incomplete bacteriophage T4 genomes. J Virol. 1976 Jun;18(3):873–884. doi: 10.1128/jvi.18.3.873-884.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kozinski A. W., Felgenhauer Z. Z. Molecular recombination in T4 bacteriophage deoxyribonucleic acid. II. Single-strand breaks and exposure of uncomplemented areas as a prerequisite for recombination. J Virol. 1967 Dec;1(6):1193–1202. doi: 10.1128/jvi.1.6.1193-1202.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kozinski A. W., Ling S. K., Hutchinson N., Halpern M. E., Mattson T. Differential amplification of specific areas of phage T4 genome as revealed by hybridization to cloned genetic segments. Proc Natl Acad Sci U S A. 1980 Sep;77(9):5064–5068. doi: 10.1073/pnas.77.9.5064. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Ling S. K., Vogelbacker H. H., Restifo L. L., Mattson T., Kozinski A. W. Partial replication of UV-irradiated T4 bacteriophage DNA results in amplification of specific genetic areas. J Virol. 1981 Nov;40(2):403–410. doi: 10.1128/jvi.40.2.403-410.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Mosig G. A map of distances along the DNA molecule of phage T4. Genetics. 1968 Jun;59(2):137–151. doi: 10.1093/genetics/59.2.137. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Murray R. E., Mathews C. K. Addition of nucleotides to parental DNA early in infection by bacteriophage T4. J Mol Biol. 1969 Sep 14;44(2):233–248. doi: 10.1016/0022-2836(69)90172-7. [DOI] [PubMed] [Google Scholar]
  12. Scofield M. S., Collinsworth W. L., Mathews C. K. Continued synthesis of bacterial DNA after infection by bacteriophage T4. J Virol. 1974 Apr;13(4):847–857. doi: 10.1128/jvi.13.4.847-857.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Velten J., Fukada K., Abelson J. In vitro construction of bacteriophage lambda and plasmid DNA molecules containing DNA fragments from bacteriophage T4. Gene. 1976;1(1):93–106. doi: 10.1016/0378-1119(76)90009-3. [DOI] [PubMed] [Google Scholar]
  14. Young E. T., Mattson T., Selzer G., Van Houwe G., Bolle A., Epstein R. Bacteriophage T4 gene transcription studied by hybridization to cloned restriction fragments. J Mol Biol. 1980 Apr 15;138(3):423–445. doi: 10.1016/s0022-2836(80)80011-8. [DOI] [PubMed] [Google Scholar]

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