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. 1981 Nov;40(2):508–515. doi: 10.1128/jvi.40.2.508-515.1981

Herpes simplex virus type 1 and 2 intracellular p40: type-specific and cross-reactive antigenic determinants on peptides generated by partial proteolysis.

C J Heilman Jr, M Zweig, B Hampar
PMCID: PMC256653  PMID: 6172597

Abstract

Intracellular p40 is a class of protein ranging in molecular weight from 39,000 to 45,000 that is immunoprecipitated from herpes simplex virus type 1 (HSV-1)- and HSV-2-infected cell extracts by mouse monoclonal antibodies or guinea pig antisera against HSV-1 and HSV-2 nucleocapsid p40. Analysis by a two-dimensional gel system showed that HSV-1 and HSV-2 intracellular p40 each consisted of three major components. However, these HSV-1 and HSV-2 proteins differed in charge and size. Analysis of Staphylococcus aureus V8 protease partial digests by two-dimensional gel electrophoresis indicated that none of the peptides of HSV-1 and HSV-2 intracellular p40 were identical. Immunoprecipitation of the partial digest products of intracellular p40-1 and p40-2 with homologous and heterologous guinea pig antisera resulted in the precipitation of various combinations of peptides indicating the presence of either type-specific or cross-reactive antigenic determinants.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ames G. F., Nikaido K. Two-dimensional gel electrophoresis of membrane proteins. Biochemistry. 1976 Feb 10;15(3):616–623. doi: 10.1021/bi00648a026. [DOI] [PubMed] [Google Scholar]
  2. Breithaupt T. B., Nystrom I. E., Hodges D. H., Jr, Babitch J. A. Composition of an isoelectric focusing gel yielding a broad pH gradient. Anal Biochem. 1978 Feb;84(2):579–582. doi: 10.1016/0003-2697(78)90078-7. [DOI] [PubMed] [Google Scholar]
  3. Cleveland D. W., Fischer S. G., Kirschner M. W., Laemmli U. K. Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis. J Biol Chem. 1977 Feb 10;252(3):1102–1106. [PubMed] [Google Scholar]
  4. Heilman C. J., Jr, Zweig M., Stephenson J. R., Hampar B. Isolation of a nucleocapsid polypeptide of herpes simplex virus types 1 and 2 possessing immunologically type-specific and cross-reactive determinants. J Virol. 1979 Jan;29(1):34–42. doi: 10.1128/jvi.29.1.34-42.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Honess R. W., Powell K. L., Robinson D. J., Sim C., Watson D. H. Type specific and type common antigens in cells infected with herpes simplex virus type 1 and on the surfaces of naked and enveloped particles of the virus. J Gen Virol. 1974 Feb;22(2):159–169. doi: 10.1099/0022-1317-22-2-159. [DOI] [PubMed] [Google Scholar]
  6. Honess R. W., Roizman B. Proteins specified by herpes simplex virus. XI. Identification and relative molar rates of synthesis of structural and nonstructural herpes virus polypeptides in the infected cell. J Virol. 1973 Dec;12(6):1347–1365. doi: 10.1128/jvi.12.6.1347-1365.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. O'Farrell P. H. High resolution two-dimensional electrophoresis of proteins. J Biol Chem. 1975 May 25;250(10):4007–4021. [PMC free article] [PubMed] [Google Scholar]
  8. O'Farrell P. H., O'Farrell P. Z. Two-dimensional polyacrylamide gel electrophoretic fractionation. Methods Cell Biol. 1977;16:407–420. doi: 10.1016/s0091-679x(08)60116-8. [DOI] [PubMed] [Google Scholar]
  9. Pereira L., Klassen T., Baringer J. R. Type-common and type-specific monoclonal antibody to herpes simplex virus type 1. Infect Immun. 1980 Aug;29(2):724–732. doi: 10.1128/iai.29.2.724-732.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Powell K. L., Courtney R. J. Polypeptide synthesized in herpes simplex virus type 2-infected HEp-2 cells. Virology. 1975 Jul;66(1):217–228. doi: 10.1016/0042-6822(75)90192-0. [DOI] [PubMed] [Google Scholar]
  11. Powell K. L., Watson D. H. Some structural antigens of herpes simplex virus type 1. J Gen Virol. 1975 Nov;29(2):167–178. doi: 10.1099/0022-1317-29-2-167. [DOI] [PubMed] [Google Scholar]
  12. Schneweis K. E., Nahmias A. J. Antigens of Herpes simplex virus type 1 and 2-immunodiffusion and inhibition passive hemagglutination studies. Z Immunitatsforsch Exp Klin Immunol. 1971 Jun;141(5):471–487. [PubMed] [Google Scholar]
  13. Sim C., Watson D. H. The role of type specific and cross reacting structural antigens in the neutralization of herpes simplex virus types 1 and 2. J Gen Virol. 1973 May;19(2):217–233. doi: 10.1099/0022-1317-19-2-217. [DOI] [PubMed] [Google Scholar]
  14. Skinner G. R., Thouless M. E., Trueman S., Edwards J., Gibbs A. J. Serological relatedness of herpes simplex viruses. Type-specificity of antibody response. Immunology. 1976 Sep;31(3):481–494. [PMC free article] [PubMed] [Google Scholar]
  15. Zweig M., Heilman C. J., Jr, Rabin H., Hampar B. Shared antigenic determinants between two distinct classes of proteins in cells infected with herpes simplex virus. J Virol. 1980 Sep;35(3):644–652. doi: 10.1128/jvi.35.3.644-652.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Zweig M., Heilman C. J., Jr, Rabin H., Hopkins R. F., 3rd, Neubauer R. H., Hampar B. Production of monoclonal antibodies against nucleocapsid proteins of herpes simplex virus types 1 and 2. J Virol. 1979 Nov;32(2):676–678. doi: 10.1128/jvi.32.2.676-678.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]

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