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. 1981 Dec;40(3):971–976. doi: 10.1128/jvi.40.3.971-976.1981

Env gene products of AKR dual-tropic viruses: examination of peptide maps and cell surface expression.

N G Famulari, K J English
PMCID: PMC256712  PMID: 6275131

Abstract

The env gene products of nine AKR dual-tropic murine leukemia viruses were compared by peptide mapping and were assayed for expression on the cell surface of infected fibroblasts. Seven virus isolates expressed the env gene polyprotein on the cell surface. The env gene products of six of the seven viruses had identical peptide maps. The analysis of structure and expression of env gene products carried out in this study characterizes a subset of dual-tropic murine leukemia viruses shown by others to be thymotropic.

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Selected References

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  1. Cleveland D. W., Fischer S. G., Kirschner M. W., Laemmli U. K. Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis. J Biol Chem. 1977 Feb 10;252(3):1102–1106. [PubMed] [Google Scholar]
  2. Cloyd M. W., Hartley J. W., Rowe W. P. Cell-surface antigens associated with recombinant mink cell focus-inducing murine leukemia viruses. J Exp Med. 1979 Mar 1;149(3):702–712. doi: 10.1084/jem.149.3.702. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cloyd M. W., Hartley J. W., Rowe W. P. Lymphomagenicity of recombinant mink cell focus-inducing murine leukemia viruses. J Exp Med. 1980 Mar 1;151(3):542–552. doi: 10.1084/jem.151.3.542. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Elder J. H., Gautsch J. W., Jensen F. C., Lerner R. A., Hartley J. W., Rowe W. P. Biochemical evidence that MCF murine leukemia viruses are envelope (env) gene recombinants. Proc Natl Acad Sci U S A. 1977 Oct;74(10):4676–4680. doi: 10.1073/pnas.74.10.4676. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Famulari N. G., Jelalian K. Cell surface expression of the env gene polyprotein of dual-tropic mink cell focus-forming murine leukemia virus. J Virol. 1979 Jun;30(3):720–728. doi: 10.1128/jvi.30.3.720-728.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Famulari N. G., Tung J. S., O'Donnell P. V., Fleissner E. Murine leukemia virus env-gene expression in preleukemic thymocytes and leukemia cells of AKR strain mice. Cold Spring Harb Symp Quant Biol. 1980;44(Pt 2):1281–1287. doi: 10.1101/sqb.1980.044.01.140. [DOI] [PubMed] [Google Scholar]
  7. Hartley J. W., Wolford N. K., Old L. J., Rowe W. P. A new class of murine leukemia virus associated with development of spontaneous lymphomas. Proc Natl Acad Sci U S A. 1977 Feb;74(2):789–792. doi: 10.1073/pnas.74.2.789. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hays E. F., Vredevoe D. L. A discrepancy in XC and oncogenicity assays for murine leukemia virus in AKR mice. Cancer Res. 1977 Mar;37(3):726–730. [PubMed] [Google Scholar]
  9. Kawashima K., Ikeda H., Stockert E., Takahashi T., Old L. J. Age-related changes in cell surface antigens of preleukemic AKR thymocytes. J Exp Med. 1976 Jul 1;144(1):193–208. doi: 10.1084/jem.144.1.193. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kemp M. C., Famulari N. G., Compans R. W. Glycoproteins of murine leukemia viruses. III. Glycosylation of env precursor glycoproteins. J Virol. 1981 Aug;39(2):463–470. doi: 10.1128/jvi.39.2.463-470.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kemp M. C., Famulari N. G., O'Donnell P. V., Compans R. W. Glycopeptides of murine leukemia viruses. II. Comparison of xenotropic and dual-tropic viruses. J Virol. 1980 Apr;34(1):154–161. doi: 10.1128/jvi.34.1.154-161.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lung M. L., Hering C., Hartley J. W., Rowe W. P., Hopkins N. Analysis of the genomes of mink cell focus-inducing murine type-C viruses: a progress report. Cold Spring Harb Symp Quant Biol. 1980;44(Pt 2):1269–1274. doi: 10.1101/sqb.1980.044.01.138. [DOI] [PubMed] [Google Scholar]
  13. Nowinski R. C., Hays E. F. Oncogenicity of AKR endogenous leukemia viruses. J Virol. 1978 Jul;27(1):13–18. doi: 10.1128/jvi.27.1.13-18.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. O'Donnell P. V., Stockert E., Obata Y., DeLeo A. B., Old L. J. Murine-leukemia-virus-related cell-surface antigens as serological markers of AKR ecotropic, xenotropic, and dualtropic viruses. Cold Spring Harb Symp Quant Biol. 1980;44(Pt 2):1255–1264. doi: 10.1101/sqb.1980.044.01.136. [DOI] [PubMed] [Google Scholar]
  15. O'Donnell P. V., Stockert E., Obata Y., Old L. J. Leukemogenic properties of AKR dualtropic (MCF) viruses: amplification of murine leukemia virus-related antigens on thymocytes and acceleration of leukemia development in AKR mice. Virology. 1981 Jul 30;112(2):548–563. doi: 10.1016/0042-6822(81)90301-9. [DOI] [PubMed] [Google Scholar]
  16. Rommelaere J., Faller D. V., Hopkins N. Characterization and mapping of RNase T1-resistant oligonucleotides derived from the genomes of Akv and MCF murine leukemia viruses. Proc Natl Acad Sci U S A. 1978 Jan;75(1):495–499. doi: 10.1073/pnas.75.1.495. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Rosner M. R., Grinna L. S., Robbins P. W. Differences in glycosylation patterns of closely related murine leukemia viruses. Proc Natl Acad Sci U S A. 1980 Jan;77(1):67–71. doi: 10.1073/pnas.77.1.67. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Stockert E., DeLeo A. B., O'Donnell P. V., Obata Y., Old L. J. G(AKSL2): a new cell surface antigen of the mouse related to the dualtropic mink cell focus-inducing class of murine leukemia virus detected by naturally occurring antibody. J Exp Med. 1979 Jan 1;149(1):200–215. doi: 10.1084/jem.149.1.200. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Tung J. S., Fleissner E. Amplified env and gag products on AKR cells. Origin from different murine leukemia virus genomes. J Exp Med. 1980 Apr 1;151(4):975–979. doi: 10.1084/jem.151.4.975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Witte O. N., Wirth D. F. Structure of the murine leukemia virus envelope glycoprotein precursor. J Virol. 1979 Feb;29(2):735–743. doi: 10.1128/jvi.29.2.735-743.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]

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