Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1982 Feb;41(2):686–691. doi: 10.1128/jvi.41.2.686-691.1982

Limited transcription of the herpes simplex virus genome when latent in human sensory ganglia.

D A Galloway, C M Fenoglio, J K McDougall
PMCID: PMC256797  PMID: 6281477

Abstract

Human paravertebral ganglia were examined for the presence of specific sequences of viral RNA by in situ cytological hybridization, using as probes individual fragments of herpes simplex virus type 2 DNA. Ganglia were obtained at autopsy from 40 patients, and 14 were positive for herpes simplex virus RNA. Transcripts from the left-hand 30% of the viral genome were detected in all of the herpes simplex virus-positive cases. RNA homologous to other sequences from the L component were present less frequently, and no RNA from the S component was detected. These results argue that specific transcription of the herpes simplex virus genome occurs in latently infected human ganglion cells.

Full text

PDF
690

Images in this article

688Image
on p.688
689Image
on p.689

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Galloway D. A., Fenoglio C., Shevchuk M., McDougall J. K. Detection of herpes simplex RNA in human sensory ganglia. Virology. 1979 May;95(1):265–268. doi: 10.1016/0042-6822(79)90429-x. [DOI] [PubMed] [Google Scholar]
  2. Galloway D. A., Swain M. Cloning of Herpes simplex virus 2 DNA fragments in a plasmid vector. Gene. 1980 Nov;11(3-4):253–257. doi: 10.1016/0378-1119(80)90065-7. [DOI] [PubMed] [Google Scholar]
  3. Lofgren K. W., Stevens J. G., Marsden H. S., Subak-Sharpe J. H. Temperature-sensitive mutants of herpes simplex virus differ in the capacity to establish latent infections in mice. Virology. 1977 Jan;76(1):440–443. doi: 10.1016/0042-6822(77)90319-1. [DOI] [PubMed] [Google Scholar]
  4. Maniatis T., Kee S. G., Efstratiadis A., Kafatos F. C. Amplification and characterization of a beta-globin gene synthesized in vitro. Cell. 1976 Jun;8(2):163–182. doi: 10.1016/0092-8674(76)90001-5. [DOI] [PubMed] [Google Scholar]
  5. McDougall J. K., Galloway D. A., Fenoglio C. M. Cervical carcinoma: detection of herpes simplex virus RNA in cells undergoing neoplastic change. Int J Cancer. 1980 Jan 15;25(1):1–8. doi: 10.1002/ijc.2910250102. [DOI] [PubMed] [Google Scholar]
  6. McDougall J. K., Masse T. H., Galloway D. A. Location and cloning of the herpes simplex virus type 2 thymidine kinase gene. J Virol. 1980 Mar;33(3):1221–1224. doi: 10.1128/jvi.33.3.1221-1224.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Preston C. M. Abnormal properties of an immediate early polypeptide in cells infected with the herpes simplex virus type 1 mutant tsK. J Virol. 1979 Nov;32(2):357–369. doi: 10.1128/jvi.32.2.357-369.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Puga A., Rosenthal J. D., Openshaw H., Notkins A. L. Herpes simplex virus DNA and mRNA sequences in acutely and chronically infected trigeminal ganglia of mice. Virology. 1978 Aug;89(1):102–111. doi: 10.1016/0042-6822(78)90044-2. [DOI] [PubMed] [Google Scholar]
  9. Rigby P. W., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. doi: 10.1016/0022-2836(77)90052-3. [DOI] [PubMed] [Google Scholar]
  10. Sekizawa T., Openshaw H., Wohlenberg C., Notkins A. L. Latency of herpes simplex virus in absence of neutralizing antibody: model for reactivation. Science. 1980 Nov 28;210(4473):1026–1028. doi: 10.1126/science.6254149. [DOI] [PubMed] [Google Scholar]
  11. Stevens J. G., Cook M. L. Latent herpes simplex virus in spinal ganglia of mice. Science. 1971 Aug 27;173(3999):843–845. doi: 10.1126/science.173.3999.843. [DOI] [PubMed] [Google Scholar]
  12. Stevens J. G., Cook M. L. Maintenance of latent herpetic infection: an apparent role for anti-viral IgG. J Immunol. 1974 Dec;113(6):1685–1693. [PubMed] [Google Scholar]
  13. Tenser R. B., Dunstan M. E. Herpes simplex virus thymidine kinase expression in infection of the trigeminal ganglion. Virology. 1979 Dec;99(2):417–422. doi: 10.1016/0042-6822(79)90021-7. [DOI] [PubMed] [Google Scholar]
  14. Walboomers J. M., Schegget J. T. A new method for the isolation of herpes simplex virus type 2 DNA. Virology. 1976 Oct 1;74(1):256–258. doi: 10.1016/0042-6822(76)90151-3. [DOI] [PubMed] [Google Scholar]
  15. Walz M. A., Price R. W., Notkins A. L. Latent ganglionic infection with herpes simplex virus types 1 and 2: viral reactivation in vivo after neurectomy. Science. 1974 Jun 14;184(4142):1185–1187. doi: 10.1126/science.184.4142.1185. [DOI] [PubMed] [Google Scholar]
  16. Warren K. G., Koprowski H., Lonsdale D. M., Brown S. M., Subak-Sharpe J. H. The polypeptide and the DNA restriction enzyme profiles of spontaneous isolates of herpes simplex virus type 1 from explants of human trigeminal, superior cervical and vagus ganglia. J Gen Virol. 1979 Apr;43(1):151–171. doi: 10.1099/0022-1317-43-1-151. [DOI] [PubMed] [Google Scholar]
  17. Watson K., Stevens J. G., Cook M. L., Subak-Sharpe J. H. Latency competence of thirteen HSV-1 temperature-sensitive mutants. J Gen Virol. 1980 Jul;49(1):149–159. doi: 10.1099/0022-1317-49-1-149. [DOI] [PubMed] [Google Scholar]
  18. Wigler M., Silverstein S., Lee L. S., Pellicer A., Cheng Y. c., Axel R. Transfer of purified herpes virus thymidine kinase gene to cultured mouse cells. Cell. 1977 May;11(1):223–232. doi: 10.1016/0092-8674(77)90333-6. [DOI] [PubMed] [Google Scholar]
  19. Wilkie N. M., Clements J. B., Boll W., Mantei N., Lonsdale D., Weissmann C. Hybrid plasmids containing an active thymidine kinase gene of Herpes simplex virus 1. Nucleic Acids Res. 1979 Oct 25;7(4):859–877. doi: 10.1093/nar/7.4.859. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Yamamoto H., Walz M. A., Notkins A. L. Viral-specific thymidine kinase in sensory ganglia of mice infected with herpes simplex virus. Virology. 1977 Feb;76(2):866–869. doi: 10.1016/0042-6822(77)90267-7. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES