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. 1982 May;42(2):730–733. doi: 10.1128/jvi.42.2.730-733.1982

Emergence of simian virus 40 variants during serial passage of plaque isolates.

L C Norkin, S M Tirrell
PMCID: PMC256899  PMID: 6283180

Abstract

Three serial passage series of simian virus 40 (SV40) in CV-1 cells were initiated by infection directly from the same wild-type plaque isolate, three series were initiated by infection with another plaque isolate, and two series were initiated with each of two other plaque isolates. Aberrant SV40 genomes were not detected in any of the passage series until after the fifty undiluted passage, and each series generated a different array of variant genomes. The results show that the variants were not present in the original plaque isolates but, instead, were randomly generated during subsequent high-input multiplicity passages. Although many of the aberrant viral genomes in each passage series contained reiterations of the SV40 origin of replication and some also contained host cell sequences, there was no indication that SV40 is predisposed toward generating any particular variant.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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