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. 1982 Jun;42(3):847–853. doi: 10.1128/jvi.42.3.847-853.1982

Characterization of two RNA polymerase activities induced by mouse hepatitis virus.

P R Brayton, M M Lai, C D Patton, S A Stohlman
PMCID: PMC256918  PMID: 6285000

Abstract

RNA-dependent RNA polymerase activity was found in mouse hepatitis virus strain A59 (MHV-A59)-infected cells. The enzyme was induced in the infected cells and could not be detected in the MHV-A59 virion. Two peaks of RNA polymerase activity, one early and the other late in infection, were detected. These polymerase activities were in temporal sequence with early and late virus-specific RNA synthesis. Both of them were found to be associated with membrane fractions. There were significant differences in the enzymatic properties of the two polymerases. The early polymerase, but not the late polymerase, could be activated by potassium ions in the absence of magnesium ions and also had a lower optimum pH than the late polymerase. It was therefore probable that the enzymes represent two different species of RNA polymerase and perform different roles in virus-specific RNA synthesis. The effects of cycloheximide on MHV-specific RNA synthesis were determined. Continuous protein synthesis was required for both early and late RNA synthesis and might also be required for shutoff of early RNA synthesis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson R., Cheley S., Haworth-Hatherell E. Comparison of polypeptides of two strains of murine hepatitis virus. Virology. 1979 Sep;97(2):492–494. doi: 10.1016/0042-6822(79)90363-5. [DOI] [PubMed] [Google Scholar]
  2. Baltimore D. Expression of animal virus genomes. Bacteriol Rev. 1971 Sep;35(3):235–241. doi: 10.1128/br.35.3.235-241.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bond C. W., Leibowitz J. L., Robb J. A. Pathogenic murine coronaviruses. II. Characterization of virus-specific proteins of murine coronaviruses JHMV and A59V. Virology. 1979 Apr 30;94(2):371–384. doi: 10.1016/0042-6822(79)90468-9. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Brayton P. R., Ganges R. G., Stohlman S. A. Host cell nuclear function and murine hepatitis virus replication. J Gen Virol. 1981 Oct;56(Pt 2):457–460. doi: 10.1099/0022-1317-56-2-457. [DOI] [PubMed] [Google Scholar]
  5. Caliguiri L. A. Analysis of RNA associated with the poliovirus RNA replication complexes. Virology. 1974 Apr;58(2):526–535. doi: 10.1016/0042-6822(74)90086-5. [DOI] [PubMed] [Google Scholar]
  6. Girard M. In vitro synthesis of poliovirus ribonucleic acid: role of the replicative intermediate. J Virol. 1969 Apr;3(4):376–384. doi: 10.1128/jvi.3.4.376-384.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Huang A. S., Baltimore D., Bratt M. A. Ribonucleic acid polymerase in virions of Newcastle disease virus: comparison with the vesicular stomatitis virus polymerase. J Virol. 1971 Mar;7(3):389–394. doi: 10.1128/jvi.7.3.389-394.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Lai M. M., Brayton P. R., Armen R. C., Patton C. D., Pugh C., Stohlman S. A. Mouse hepatitis virus A59: mRNA structure and genetic localization of the sequence divergence from hepatotropic strain MHV-3. J Virol. 1981 Sep;39(3):823–834. doi: 10.1128/jvi.39.3.823-834.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lai M. M., Stohlman S. A. Comparative analysis of RNA genomes of mouse hepatitis viruses. J Virol. 1981 May;38(2):661–670. doi: 10.1128/jvi.38.2.661-670.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Lai M. M., Stohlman S. A. RNA of mouse hepatitis virus. J Virol. 1978 May;26(2):236–242. doi: 10.1128/jvi.26.2.236-242.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Michel M. R., Gomatos P. J. Semliki forest virus-specific RNAs synthesized in vitro by enzyme from infected BHK cells. J Virol. 1973 Jun;11(6):900–914. doi: 10.1128/jvi.11.6.900-914.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Polatnick J., Arlinghaus R. B. Foot-and-mouth disease virus-induced ribonucleic acid polymerase in baby hamster kidney cells. Virology. 1967 Apr;31(4):601–608. doi: 10.1016/0042-6822(67)90188-2. [DOI] [PubMed] [Google Scholar]
  13. Segal S., Sreevalsan T. Sindbis virus replicative intermediates: purification and characterization. Virology. 1974 Jun;59(2):428–442. [PubMed] [Google Scholar]
  14. Siddell S., Wege H., Barthel A., ter Meulen V. Coronavirus JHM: intracellular protein synthesis. J Gen Virol. 1981 Mar;53(Pt 1):145–155. doi: 10.1099/0022-1317-53-1-145. [DOI] [PubMed] [Google Scholar]
  15. Spaan W. J., Rottier P. J., Horzinek M. C., van der Zeijst B. A. Isolation and identification of virus-specific mRNAs in cells infected with mouse hepatitis virus (MHV-A59). Virology. 1981 Jan 30;108(2):424–434. doi: 10.1016/0042-6822(81)90449-9. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Sreevalsan T., Yin F. H. Sindbis virus-induced viral ribonucleic acid polymerase. J Virol. 1969 Jun;3(6):599–604. doi: 10.1128/jvi.3.6.599-604.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Sturman L. S. I. Structural proteins: effects of preparative conditions on the migration of protein in polyacrylamide gels. Virology. 1977 Apr;77(2):637–649. doi: 10.1016/0042-6822(77)90488-3. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Tyrrell D. A., Alexander D. J., Almeida J. D., Cunningham C. H., Easterday B. C., Garwes D. J., Hierholzer J. C., Kapikian A., Macnaughton M. R., McIntosh K. Coronaviridae: second report. Intervirology. 1978;10(6):321–328. doi: 10.1159/000148996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Wege H., Müller A., ter Meulen V. Genomic RNA of the murine coronavirus JHM. J Gen Virol. 1978 Nov;41(2):217–227. doi: 10.1099/0022-1317-41-2-217. [DOI] [PubMed] [Google Scholar]
  20. Wege H., Siddell S., Sturm M., Ter Meulen V. Coronavirus JHM: characterization of intracellular viral RNA. J Gen Virol. 1981 May;54(Pt 1):213–217. doi: 10.1099/0022-1317-54-1-213. [DOI] [PubMed] [Google Scholar]
  21. Wilhelmsen K. C., Leibowitz J. L., Bond C. W., Robb J. A. The replication of murine coronaviruses in enucleated cells. Virology. 1981 Apr 15;110(1):225–230. doi: 10.1016/0042-6822(81)90027-1. [DOI] [PMC free article] [PubMed] [Google Scholar]

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