Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1992 Jul;60(7):3011–3014. doi: 10.1128/iai.60.7.3011-3014.1992

Survival of virulent and attenuated strains of Brucella abortus in normal and gamma interferon-activated murine peritoneal macrophages.

S M Jones 1, A J Winter 1
PMCID: PMC257269  PMID: 1612769

Abstract

Virulent Brucella abortus 2308 was phagocytized more readily than attenuated strain 19 following opsonization and survived at significantly higher levels in normal murine peritoneal macrophages and in macrophages treated with gamma interferon. Activation of macrophages with gamma interferon greatly inhibited intracellular replication of strain 2308 but did not result in its elimination. These data support the hypothesis that persistent infection of the host requires the ability of antibody-opsonized B. abortus to survive in activated macrophages.

Full text

PDF
3013

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ackermann M. R., Cheville N. F., Deyoe B. L. Bovine ileal dome lymphoepithelial cells: endocytosis and transport of Brucella abortus strain 19. Vet Pathol. 1988 Jan;25(1):28–35. doi: 10.1177/030098588802500104. [DOI] [PubMed] [Google Scholar]
  2. Anderson T. D., Cheville N. F., Meador V. P. Pathogenesis of placentitis in the goat inoculated with Brucella abortus. II. Ultrastructural studies. Vet Pathol. 1986 May;23(3):227–239. doi: 10.1177/030098588602300302. [DOI] [PubMed] [Google Scholar]
  3. Anderson T. D., Cheville N. F. Ultrastructural morphometric analysis of Brucella abortus-infected trophoblasts in experimental placentitis. Bacterial replication occurs in rough endoplasmic reticulum. Am J Pathol. 1986 Aug;124(2):226–237. [PMC free article] [PubMed] [Google Scholar]
  4. Araya L. N., Winter A. J. Comparative protection of mice against virulent and attenuated strains of Brucella abortus by passive transfer of immune T cells or serum. Infect Immun. 1990 Jan;58(1):254–256. doi: 10.1128/iai.58.1.254-256.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bertram T. A., Canning P. C., Roth J. A. Preferential inhibition of primary granule release from bovine neutrophils by a Brucella abortus extract. Infect Immun. 1986 Apr;52(1):285–292. doi: 10.1128/iai.52.1.285-292.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Dees C., Fountain M. W., Taylor J. R., Schultz R. D. Enhanced intraphagocytic killing of Brucella abortus in bovine mononuclear cells by liposomes-containing gentamicin. Vet Immunol Immunopathol. 1985 Jan;8(1-2):171–182. doi: 10.1016/0165-2427(85)90120-5. [DOI] [PubMed] [Google Scholar]
  7. Detilleux P. G., Cheville N. F., Deyoe B. L. Pathogenesis of Brucella abortus in chicken embryos. Vet Pathol. 1988 Mar;25(2):138–146. doi: 10.1177/030098588802500206. [DOI] [PubMed] [Google Scholar]
  8. Detilleux P. G., Deyoe B. L., Cheville N. F. Entry and intracellular localization of Brucella spp. in Vero cells: fluorescence and electron microscopy. Vet Pathol. 1990 Sep;27(5):317–328. doi: 10.1177/030098589002700503. [DOI] [PubMed] [Google Scholar]
  9. Detilleux P. G., Deyoe B. L., Cheville N. F. Penetration and intracellular growth of Brucella abortus in nonphagocytic cells in vitro. Infect Immun. 1990 Jul;58(7):2320–2328. doi: 10.1128/iai.58.7.2320-2328.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Enright F. M., Araya L. N., Elzer P. H., Rowe G. E., Winter A. J. Comparative histopathology in BALB/c mice infected with virulent and attenuated strains of Brucella abortus. Vet Immunol Immunopathol. 1990 Oct;26(2):171–182. doi: 10.1016/0165-2427(90)90065-z. [DOI] [PubMed] [Google Scholar]
  11. Frenchick P. J., Markham R. J., Cochrane A. H. Inhibition of phagosome-lysosome fusion in macrophages by soluble extracts of virulent Brucella abortus. Am J Vet Res. 1985 Feb;46(2):332–335. [PubMed] [Google Scholar]
  12. Harmon B. G., Adams L. G., Frey M. Survival of rough and smooth strains of Brucella abortus in bovine mammary gland macrophages. Am J Vet Res. 1988 Jul;49(7):1092–1097. [PubMed] [Google Scholar]
  13. Kreutzer D. L., Dreyfus L. A., Robertson D. C. Interaction of polymorphonuclear leukocytes with smooth and rough strains of Brucella abortus. Infect Immun. 1979 Mar;23(3):737–742. doi: 10.1128/iai.23.3.737-742.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Meador V. P., Deyoe B. L. Intracellular localization of Brucella abortus in bovine placenta. Vet Pathol. 1989 Nov;26(6):513–515. doi: 10.1177/030098588902600609. [DOI] [PubMed] [Google Scholar]
  15. Montaraz J. A., Winter A. J. Comparison of living and nonliving vaccines for Brucella abortus in BALB/c mice. Infect Immun. 1986 Aug;53(2):245–251. doi: 10.1128/iai.53.2.245-251.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Murray H. W., Spitalny G. L., Nathan C. F. Activation of mouse peritoneal macrophages in vitro and in vivo by interferon-gamma. J Immunol. 1985 Mar;134(3):1619–1622. [PubMed] [Google Scholar]
  17. Nesthus I., Haneberg B., Glette J., Solberg C. O. The influence of antimicrobial agents on macrophage-associated Staphylococcus aureus. Acta Pathol Microbiol Immunol Scand B. 1985 Jun;93(3):189–194. doi: 10.1111/j.1699-0463.1985.tb02875.x. [DOI] [PubMed] [Google Scholar]
  18. Price R. E., Templeton J. W., Adams L. G. Survival of smooth, rough and transposon mutant strains of Brucella abortus in bovine mammary macrophages. Vet Immunol Immunopathol. 1990 Dec;26(4):353–365. doi: 10.1016/0165-2427(90)90119-d. [DOI] [PubMed] [Google Scholar]
  19. Tilney L. G., Portnoy D. A. Actin filaments and the growth, movement, and spread of the intracellular bacterial parasite, Listeria monocytogenes. J Cell Biol. 1989 Oct;109(4 Pt 1):1597–1608. doi: 10.1083/jcb.109.4.1597. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Winter A. J., Duncan J. R., Santisteban C. G., Douglas J. T., Adams L. G. Capacity of passively administered antibody to prevent establishment of Brucella abortus infection in mice. Infect Immun. 1989 Nov;57(11):3438–3444. doi: 10.1128/iai.57.11.3438-3444.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Young E. J., Borchert M., Kretzer F. L., Musher D. M. Phagocytosis and killing of Brucella by human polymorphonuclear leukocytes. J Infect Dis. 1985 Apr;151(4):682–690. doi: 10.1093/infdis/151.4.682. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES