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. 1992 Sep;60(9):3921–3924. doi: 10.1128/iai.60.9.3921-3924.1992

Identification of an acute-phase reactant in murine infections with Trypanosoma brucei.

S Z Shapiro 1, S J Black 1
PMCID: PMC257411  PMID: 1500201

Abstract

A 42-kDa protein appeared at a much higher concentration in plasma from Trypanosoma brucei-resistant (C57BL/6) mice after infection than in plasma from trypanosome-susceptible (C3H/He) mice. This protein was purified by sequential steps of gel filtration, protein A-Sepharose affinity chromatography, isoelectric focusing, and ammonium sulfate precipitation. The purified protein was identified as a subunit of the acute-phase reactant haptoglobin. Causes of elevated plasma haptoglobin and its implications for resistance to trypanosomiasis are discussed.

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Selected References

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  1. Baumann H., Jahreis G. P., Morella K. K., Won K. A., Pruitt S. C., Jones V. E., Prowse K. R. Transcriptional regulation through cytokine and glucocorticoid response elements of rat acute phase plasma protein genes by C/EBP and JunB. J Biol Chem. 1991 Oct 25;266(30):20390–20399. [PubMed] [Google Scholar]
  2. Baumann H., Morella K. K., Jahreis G. P., Marinković S. Distinct regulation of the interleukin-1 and interleukin-6 response elements of the rat haptoglobin gene in rat and human hepatoma cells. Mol Cell Biol. 1990 Nov;10(11):5967–5976. doi: 10.1128/mcb.10.11.5967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Baumann H., Prowse K. R., Marinković S., Won K. A., Jahreis G. P. Stimulation of hepatic acute phase response by cytokines and glucocorticoids. Ann N Y Acad Sci. 1989;557:280-95, discussion 295-6. doi: 10.1111/j.1749-6632.1989.tb24021.x. [DOI] [PubMed] [Google Scholar]
  4. Baumann H., Schendel P. Interleukin-11 regulates the hepatic expression of the same plasma protein genes as interleukin-6. J Biol Chem. 1991 Oct 25;266(30):20424–20427. [PubMed] [Google Scholar]
  5. Black S. J., Sendashonga C. N., Webster P., Koch G. L., Shapiro S. Z. Regulation of parasite-specific antibody responses in resistant (C57BL/6) and susceptible (C3H/HE) mice infected with Trypanosoma (trypanozoon) brucei brucei. Parasite Immunol. 1986 Sep;8(5):425–442. doi: 10.1111/j.1365-3024.1986.tb00859.x. [DOI] [PubMed] [Google Scholar]
  6. Burnette W. N. "Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate--polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Anal Biochem. 1981 Apr;112(2):195–203. doi: 10.1016/0003-2697(81)90281-5. [DOI] [PubMed] [Google Scholar]
  7. Clayton C. E., Ogilvie B. M., Askonas B. A. Trypanosoma brucei infection in nude mice: B lymphocyte function is suppressed in the absence of T lymphocytes. Parasite Immunol. 1979 Spring;1(1):39–48. doi: 10.1111/j.1365-3024.1979.tb00694.x. [DOI] [PubMed] [Google Scholar]
  8. Esievo K. A., Saror D. I., Adegoke O. O. Depleted serum haptoglobin in acute bovine trypanosomiasis. Vet Parasitol. 1984 Sep;15(3-4):181–185. doi: 10.1016/0304-4017(84)90070-0. [DOI] [PubMed] [Google Scholar]
  9. Greenblatt H. C., Diggs C. L., Rosenstreich D. L. Trypanosoma rhodesiense: analysis of the genetic control of resistance among mice. Infect Immun. 1984 Apr;44(1):107–111. doi: 10.1128/iai.44.1.107-111.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Jones J. F., Hancock G. E. Trypanosomiasis in mice with naturally occurring immunodeficiencies. Infect Immun. 1983 Nov;42(2):848–851. doi: 10.1128/iai.42.2.848-851.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Natsuka S., Isshiki H., Akira S., Kishimoto T. Augmentation of haptoglobin production in Hep3B cell line by a nuclear factor NF-IL6. FEBS Lett. 1991 Oct 7;291(1):58–62. doi: 10.1016/0014-5793(91)81103-f. [DOI] [PubMed] [Google Scholar]
  12. Oh S. K., Kim S. H., Walker J. E. Interference with immune response at the level of generating effector cells by tumor-associated haptoglobin. J Natl Cancer Inst. 1990 Jun 6;82(11):934–940. doi: 10.1093/jnci/82.11.934. [DOI] [PubMed] [Google Scholar]
  13. Pepys M. B., Baltz M. L. Acute phase proteins with special reference to C-reactive protein and related proteins (pentaxins) and serum amyloid A protein. Adv Immunol. 1983;34:141–212. doi: 10.1016/s0065-2776(08)60379-x. [DOI] [PubMed] [Google Scholar]
  14. Prowse K. R., Baumann H. Interleukin-1 and interleukin-6 stimulate acute-phase protein production in primary mouse hepatocytes. J Leukoc Biol. 1989 Jan;45(1):55–61. doi: 10.1002/jlb.45.1.55. [DOI] [PubMed] [Google Scholar]
  15. Shapiro S. Z., Fujisaki K., Morzaria S. P., Webster P., Fujinaga T., Spooner P. R., Irvin A. D. A life-cycle stage-specific antigen of Theileria parva recognized by anti-macroschizont monoclonal antibodies. Parasitology. 1987 Feb;94(Pt 1):29–37. doi: 10.1017/s0031182000053427. [DOI] [PubMed] [Google Scholar]
  16. Sileghem M., Makumyaviri A. M., Le Ray D., Hamers R., De Baetselier P. Modulation of lymphokine production during experimental Trypanosoma brucei infection in inbred mouse strains. Ann Soc Belg Med Trop. 1987;67(2):129–135. [PubMed] [Google Scholar]
  17. Webster P., Shapiro S. Z. Trypanosoma brucei: a membrane-associated protein in coated endocytotic vesicles. Exp Parasitol. 1990 Feb;70(2):154–163. doi: 10.1016/0014-4894(90)90096-u. [DOI] [PubMed] [Google Scholar]
  18. de Gee A. L., Sonnenfeld G., Mansfield J. M. Genetics of resistance to the African trypanosomes. V. Qualitative and quantitative differences in interferon production among susceptible and resistant mouse strains. J Immunol. 1985 Apr;134(4):2723–2726. [PubMed] [Google Scholar]

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