Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1992 Oct;60(10):4051–4058. doi: 10.1128/iai.60.10.4051-4058.1992

Adhesion to and invasion of cultured human cells by Bartonella bacilliformis.

E M Hill 1, A Raji 1, M S Valenzuela 1, F Garcia 1, R Hoover 1
PMCID: PMC257435  PMID: 1398917

Abstract

Bartonella bacilliformis was tested for its ability to adhere to and invade tissue culture cell monolayers. The parasite was able to efficiently bind and penetrate human dermal fibroblasts, human laryngeal epithelium, and human umbilical vein endothelial cells. Exposure of the organism to immune serum prepared against a crude Bartonella extract containing cell wall and membranous material resulted in decreased ability of the parasite to invade host cells. There was also an overall reduction in the invasiveness of bartonellae and total host cell association when human laryngeal epithelial cells and human umbilical vein endothelial cells were preexposed to cytochalasin D, indicating an active involvement of host cells in the uptake of bartonellae. Transmission electron microscopy revealed the presence of bartonellae inside and outside intracellular vacuoles. These data suggest that a surface-associated factor is involved in the invasion process and that internalization of the parasite by host cells involves a microfilament-dependent process similar to phagocytosis.

Full text

PDF
4058

Images in this article

4053Image
on p.4053
4054Image
on p.4054
4055Image
on p.4055
4056Image
on p.4056

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Benson L. A., Kar S., McLaughlin G., Ihler G. M. Entry of Bartonella bacilliformis into erythrocytes. Infect Immun. 1986 Nov;54(2):347–353. doi: 10.1128/iai.54.2.347-353.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Clerc P., Baudry B., Sansonetti P. J. Molecular mechanisms of entry, intracellular multiplication and killing of host cells by shigellae. Curr Top Microbiol Immunol. 1988;138:3–13. [PubMed] [Google Scholar]
  3. Clerc P., Sansonetti P. J. Entry of Shigella flexneri into HeLa cells: evidence for directed phagocytosis involving actin polymerization and myosin accumulation. Infect Immun. 1987 Nov;55(11):2681–2688. doi: 10.1128/iai.55.11.2681-2688.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cuadra M., Takano J. The relationship of Bartonella bacilliformis to the red blood cell as revealed by electron microscopy. Blood. 1969 May;33(5):708–716. [PubMed] [Google Scholar]
  5. Elsinghorst E. A., Baron L. S., Kopecko D. J. Penetration of human intestinal epithelial cells by Salmonella: molecular cloning and expression of Salmonella typhi invasion determinants in Escherichia coli. Proc Natl Acad Sci U S A. 1989 Jul;86(13):5173–5177. doi: 10.1073/pnas.86.13.5173. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Finlay B. B., Falkow S. Common themes in microbial pathogenicity. Microbiol Rev. 1989 Jun;53(2):210–230. doi: 10.1128/mr.53.2.210-230.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Finlay B. B., Falkow S. Comparison of the invasion strategies used by Salmonella cholerae-suis, Shigella flexneri and Yersinia enterocolitica to enter cultured animal cells: endosome acidification is not required for bacterial invasion or intracellular replication. Biochimie. 1988 Aug;70(8):1089–1099. doi: 10.1016/0300-9084(88)90271-4. [DOI] [PubMed] [Google Scholar]
  8. Galán J. E., Curtiss R., 3rd Cloning and molecular characterization of genes whose products allow Salmonella typhimurium to penetrate tissue culture cells. Proc Natl Acad Sci U S A. 1989 Aug;86(16):6383–6387. doi: 10.1073/pnas.86.16.6383. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hale T. L., Morris R. E., Bonventre P. F. Shigella infection of henle intestinal epithelial cells: role of the host cell. Infect Immun. 1979 Jun;24(3):887–894. doi: 10.1128/iai.24.3.887-894.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Isberg R. R., Falkow S. A single genetic locus encoded by Yersinia pseudotuberculosis permits invasion of cultured animal cells by Escherichia coli K-12. Nature. 1985 Sep 19;317(6034):262–264. doi: 10.1038/317262a0. [DOI] [PubMed] [Google Scholar]
  11. Jaffe E. A., Nachman R. L., Becker C. G., Minick C. R. Culture of human endothelial cells derived from umbilical veins. Identification by morphologic and immunologic criteria. J Clin Invest. 1973 Nov;52(11):2745–2756. doi: 10.1172/JCI107470. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kihlström E., Nilsson L. Endocytosis of Salmonella typhimurium 395 MS and MR10 by HeLa cells. Acta Pathol Microbiol Scand B. 1977 Oct;85B(5):322–328. doi: 10.1111/j.1699-0463.1977.tb01982.x. [DOI] [PubMed] [Google Scholar]
  13. Mernaugh G., Ihler G. M. Deformation factor: an extracellular protein synthesized by Bartonella bacilliformis that deforms erythrocyte membranes. Infect Immun. 1992 Mar;60(3):937–943. doi: 10.1128/iai.60.3.937-943.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Miller V. L., Falkow S. Evidence for two genetic loci in Yersinia enterocolitica that can promote invasion of epithelial cells. Infect Immun. 1988 May;56(5):1242–1248. doi: 10.1128/iai.56.5.1242-1248.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Pierson D. E., Falkow S. Nonpathogenic isolates of Yersinia enterocolitica do not contain functional inv-homologous sequences. Infect Immun. 1990 Apr;58(4):1059–1064. doi: 10.1128/iai.58.4.1059-1064.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. REYNAFARJE C., RAMOS J. The hemolytic anemia of human bartonellosis. Blood. 1961 May;17:562–578. [PubMed] [Google Scholar]
  17. Sansonetti P. J., Kopecko D. J., Formal S. B. Involvement of a plasmid in the invasive ability of Shigella flexneri. Infect Immun. 1982 Mar;35(3):852–860. doi: 10.1128/iai.35.3.852-860.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Small P. L., Falkow S. Identification of regions on a 230-kilobase plasmid from enteroinvasive Escherichia coli that are required for entry into HEp-2 cells. Infect Immun. 1988 Jan;56(1):225–229. doi: 10.1128/iai.56.1.225-229.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Walker T. S., Winkler H. H. Bartonella bacilliformis: colonial types and erythrocyte adherence. Infect Immun. 1981 Jan;31(1):480–486. doi: 10.1128/iai.31.1.480-486.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Walker T. S., Winkler H. H. Penetration of cultured mouse fibroblasts (L cells) by Rickettsia prowazeki. Infect Immun. 1978 Oct;22(1):200–208. doi: 10.1128/iai.22.1.200-208.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES