Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1992 Jan;60(1):8–12. doi: 10.1128/iai.60.1.8-12.1992

Expression of the C3d-binding protein (CR2) from Candida albicans during experimental candidiasis as measured by lymphoblastogenesis.

M Fukayama 1, E Wadsworth 1, R Calderone 1
PMCID: PMC257495  PMID: 1370279

Abstract

The complement C3d-binding protein (CR2) of Candida albicans has been purified by immunoaffinity chromatography, and its specificity has been characterized by immunoblotting with monoclonal antibodies to the C. albicans CR2 and the mammalian CR2. Recent studies with immunoelectron microscopy indicated that the CR2 was expressed during a systemic infection in a murine model of candidiasis. As a continuation of these observations, the immunogenicity of the C. albicans CR2 was investigated in a lymphoblastogenesis assay. Lymph node cells as well as splenic lymphocytes from mice infected subcutaneously with viable blastoconidia of C. albicans reacted to the C. albicans CR2 to a significantly greater extent than did lymphocytes from uninfected mice (P less than 0.01). The maximum stimulation of splenic lymphocytes by the purified receptor occurred at a concentration of 0.54 micrograms of protein per ml after 72 h of incubation of lymphocytes and receptor. Also, splenocytes from infected or CR2-immunized mice exhibited significantly reduced responses to the T-cell-dependent mitogen phytohemagglutinin (P less than 0.01). These data indicate that lymphocytes from infected mice respond to the C. albicans CR2 in a lymphoproliferation assay to a greater extent than do lymphocytes from uninfected mice, indicating that the CR2 is expressed in vivo.

Full text

PDF
12

Images in this article

10Image
on p.10

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Calderone R. A., Linehan L., Wadsworth E., Sandberg A. L. Identification of C3d receptors on Candida albicans. Infect Immun. 1988 Jan;56(1):252–258. doi: 10.1128/iai.56.1.252-258.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Canales L., Middlemas R. O., 3rd, Louro J. M., South M. A. Immunological observations in chronic mucocutaneous candidiasis. Lancet. 1969 Sep 13;2(7620):567–571. doi: 10.1016/s0140-6736(69)90264-5. [DOI] [PubMed] [Google Scholar]
  3. Cuff C. F., Rogers C. M., Lamb B. J., Rogers T. J. Induction of suppressor cells in vitro by Candida albicans. Cell Immunol. 1986 Jun;100(1):47–56. doi: 10.1016/0008-8749(86)90005-5. [DOI] [PubMed] [Google Scholar]
  4. Cuff C. F., Taub D. D., Rogers T. J. The induction of T-suppressor cells with a soluble extract of Candida albicans. Cell Immunol. 1989 Aug;122(1):71–82. doi: 10.1016/0008-8749(89)90149-4. [DOI] [PubMed] [Google Scholar]
  5. Domer J. E., Garner R. E., Befidi-Mengue R. N. Mannan as an antigen in cell-mediated immunity (CMI) assays and as a modulator of mannan-specific CMI. Infect Immun. 1989 Mar;57(3):693–700. doi: 10.1128/iai.57.3.693-700.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Durandy A., Fischer A., Le Deist F., Drouhet E., Griscelli C. Mannan-specific and mannan-induced T-cell suppressive activity in patients with chronic mucocutaneous candidiasis. J Clin Immunol. 1987 Sep;7(5):400–409. doi: 10.1007/BF00917018. [DOI] [PubMed] [Google Scholar]
  7. Edwards J. E., Jr, Gaither T. A., O'Shea J. J., Rotrosen D., Lawley T. J., Wright S. A., Frank M. M., Green I. Expression of specific binding sites on Candida with functional and antigenic characteristics of human complement receptors. J Immunol. 1986 Dec 1;137(11):3577–3583. [PubMed] [Google Scholar]
  8. Eigentler A., Schulz T. F., Larcher C., Breitwieser E. M., Myones B. L., Petzer A. L., Dierich M. P. C3bi-binding protein on Candida albicans: temperature-dependent expression and relationship to human complement receptor type 3. Infect Immun. 1989 Feb;57(2):616–622. doi: 10.1128/iai.57.2.616-622.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Fischer A., Ballet J. J., Griscelli C. Specific inhibition of in vitro Candida-induced lymphocyte proliferation by polysaccharidic antigens present in the serum of patients with chronic mucocutaneous candidiasis. J Clin Invest. 1978 Nov;62(5):1005–1013. doi: 10.1172/JCI109204. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Gilmore B. J., Retsinas E. M., Lorenz J. S., Hostetter M. K. An iC3b receptor on Candida albicans: structure, function, and correlates for pathogenicity. J Infect Dis. 1988 Jan;157(1):38–46. doi: 10.1093/infdis/157.1.38. [DOI] [PubMed] [Google Scholar]
  11. Hawkes R. Identification of concanavalin A-binding proteins after sodium dodecyl sulfate--gel electrophoresis and protein blotting. Anal Biochem. 1982 Jun;123(1):143–146. doi: 10.1016/0003-2697(82)90634-0. [DOI] [PubMed] [Google Scholar]
  12. Heidenreich F., Dierich M. P. Candida albicans and Candida stellatoidea, in contrast to other Candida species, bind iC3b and C3d but not C3b. Infect Immun. 1985 Nov;50(2):598–600. doi: 10.1128/iai.50.2.598-600.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kanbe T., Li R. K., Wadsworth E., Calderone R. A., Cutler J. E. Evidence for expression of the C3d receptor of Candida albicans in vitro and in vivo obtained by immunofluorescence and immunoelectron microscopy. Infect Immun. 1991 May;59(5):1832–1838. doi: 10.1128/iai.59.5.1832-1838.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kirkpatrick C. H., Smith T. K. Chronic mucocutaneous candidiasis: immunologic and antibiotic therapy. Ann Intern Med. 1974 Mar;80(3):310–320. doi: 10.7326/0003-4819-80-3-310. [DOI] [PubMed] [Google Scholar]
  15. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  16. Lee K. L., Buckley H. R., Campbell C. C. An amino acid liquid synthetic medium for the development of mycelial and yeast forms of Candida Albicans. Sabouraudia. 1975 Jul;13(2):148–153. doi: 10.1080/00362177585190271. [DOI] [PubMed] [Google Scholar]
  17. Linehan L., Wadsworth E., Calderone R. Candida albicans C3d receptor, isolated by using a monoclonal antibody. Infect Immun. 1988 Aug;56(8):1981–1986. doi: 10.1128/iai.56.8.1981-1986.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Manning M., Mitchell T. G. Analysis of cytoplasmic antigens of the yeast and mycelial phases of Candida albicans by two-dimensional electrophoresis. Infect Immun. 1980 Nov;30(2):484–495. doi: 10.1128/iai.30.2.484-495.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Piccolella E., Lombardi G., Morelli R. Generation of suppressor cells in the response of human lymphocytes to a polysaccharide from Candida albicans. J Immunol. 1981 Jun;126(6):2151–2155. [PubMed] [Google Scholar]
  20. Rivas V., Rogers T. J. Studies on the cellular nature of Candida albicans-induced suppression. J Immunol. 1983 Jan;130(1):376–379. [PubMed] [Google Scholar]
  21. Rogers T. J., Balish E. Effect of systemic candidiasis on blastogenesis of lymphocytes from germfree and conventional rats. Infect Immun. 1978 Apr;20(1):142–150. doi: 10.1128/iai.20.1.142-150.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Rogers T. J., Balish E. Suppression of lymphocyte blastogenesis by Candida albicans. Clin Immunol Immunopathol. 1978 Jul;10(3):298–305. doi: 10.1016/0090-1229(78)90185-x. [DOI] [PubMed] [Google Scholar]
  23. Saxena A., Calderone R. Purification and characterization of the extracellular C3d-binding protein of Candida albicans. Infect Immun. 1990 Feb;58(2):309–314. doi: 10.1128/iai.58.2.309-314.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Smail E. H., Jones J. M. Demonstration and solubilization of antigens expressed primarily on the surfaces of Candida albicans germ tubes. Infect Immun. 1984 Jul;45(1):74–81. doi: 10.1128/iai.45.1.74-81.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Tronchin G., Bouchara J. P., Robert R. Dynamic changes of the cell wall surface of Candida albicans associated with germination and adherence. Eur J Cell Biol. 1989 Dec;50(2):285–290. [PubMed] [Google Scholar]
  26. Tronchin G., Bouchara J. P., Robert R., Senet J. M. Adherence of Candida albicans germ tubes to plastic: ultrastructural and molecular studies of fibrillar adhesins. Infect Immun. 1988 Aug;56(8):1987–1993. doi: 10.1128/iai.56.8.1987-1993.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Whelan W. L., Delga J. M., Wadsworth E., Walsh T. J., Kwon-Chung K. J., Calderone R., Lipke P. N. Isolation and characterization of cell surface mutants of Candida albicans. Infect Immun. 1990 Jun;58(6):1552–1557. doi: 10.1128/iai.58.6.1552-1557.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES