Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1991 Apr;59(4):1387–1393. doi: 10.1128/iai.59.4.1387-1393.1991

Recombinant derivatives of Pasteurella multocida toxin: candidates for a vaccine against progressive atrophic rhinitis.

S K Petersen 1, N T Foged 1, A Bording 1, J P Nielsen 1, H K Riemann 1, P L Frandsen 1
PMCID: PMC257854  PMID: 1706320

Abstract

Potential vaccine components for protection against atrophic rhinitis in pigs were developed. This was achieved by deletion mutagenesis of the gene encoding the Pasteurella multocida toxin. Four purified toxin derivatives lacking different and widely separated regions in the amino acid sequence were characterized by a lack of toxic activity. One such component was shown to induce efficient protection of vaccinated female mice and their offspring against challenge with purified P. multocida toxin.

Full text

PDF

Images in this article

1390Image
on p.1390
1390Image
on p.1390

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Buys W. E., Smith H. E., Kamps A. M., Kamp E. M., Smits M. A. Sequence of the dermonecrotic toxin of Pasteurella multocida ssp. multocida. Nucleic Acids Res. 1990 May 11;18(9):2815–2816. doi: 10.1093/nar/18.9.2815. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Chanter N., Rutter J. M., Mackenzie A. Partial purification of an osteolytic toxin from Pasteurella multocida. J Gen Microbiol. 1986 Apr;132(4):1089–1097. doi: 10.1099/00221287-132-4-1089. [DOI] [PubMed] [Google Scholar]
  3. Cheville N. F., Rimler R. B., Thurston J. R. A toxin from Pasteurella multocida type D causes acute hepatic necrosis in pigs. Vet Pathol. 1988 Nov;25(6):518–520. doi: 10.1177/030098588802500617. [DOI] [PubMed] [Google Scholar]
  4. Foged N. T., Nielsen J. P., Jorsal S. E. Protection against progressive atrophic rhinitis by vaccination with Pasteurella multocida toxin purified by monoclonal antibodies. Vet Rec. 1989 Jul 1;125(1):7–11. doi: 10.1136/vr.125.1.7. [DOI] [PubMed] [Google Scholar]
  5. Foged N. T. Quantitation and purification of the Pasteurella multocida toxin by using monoclonal antibodies. Infect Immun. 1988 Aug;56(8):1901–1906. doi: 10.1128/iai.56.8.1901-1906.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kamps A. M., Kamp E. M., Smits M. A. Cloning and expression of the dermonecrotic toxin gene of Pasteurella multocida ssp. multocida in Escherichia coli. FEMS Microbiol Lett. 1990 Jan 15;55(1-2):187–190. doi: 10.1016/0378-1097(90)90192-s. [DOI] [PubMed] [Google Scholar]
  7. Larsen J. E., Gerdes K., Light J., Molin S. Low-copy-number plasmid-cloning vectors amplifiable by derepression of an inserted foreign promoter. Gene. 1984 Apr;28(1):45–54. doi: 10.1016/0378-1119(84)90086-6. [DOI] [PubMed] [Google Scholar]
  8. Lax A. J., Chanter N. Cloning of the toxin gene from Pasteurella multocida and its role in atrophic rhinitis. J Gen Microbiol. 1990 Jan;136(1):81–87. doi: 10.1099/00221287-136-1-81. [DOI] [PubMed] [Google Scholar]
  9. Morrissey J. H. Silver stain for proteins in polyacrylamide gels: a modified procedure with enhanced uniform sensitivity. Anal Biochem. 1981 Nov 1;117(2):307–310. doi: 10.1016/0003-2697(81)90783-1. [DOI] [PubMed] [Google Scholar]
  10. Nakai T., Sawata A., Kume K. Intracellular locations of dermonecrotic toxins in Pasteurella multocida and in Bordetella bronchiseptica. Am J Vet Res. 1985 Apr;46(4):870–874. [PubMed] [Google Scholar]
  11. Pedersen K. B., Elling F. The pathogenesis of atrophic rhinitis in pigs induced by toxigenic Pasteurella multocida. J Comp Pathol. 1984 Apr;94(2):203–214. doi: 10.1016/0021-9975(84)90041-0. [DOI] [PubMed] [Google Scholar]
  12. Petersen S. K., Foged N. T. Cloning and expression of the Pasteurella multocida toxin gene, toxA, in Escherichia coli. Infect Immun. 1989 Dec;57(12):3907–3913. doi: 10.1128/iai.57.12.3907-3913.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Petersen S. K. The complete nucleotide sequence of the Pasteurella multocida toxin gene and evidence for a transcriptional repressor, TxaR. Mol Microbiol. 1990 May;4(5):821–830. doi: 10.1111/j.1365-2958.1990.tb00652.x. [DOI] [PubMed] [Google Scholar]
  14. Rozengurt E., Higgins T., Chanter N., Lax A. J., Staddon J. M. Pasteurella multocida toxin: potent mitogen for cultured fibroblasts. Proc Natl Acad Sci U S A. 1990 Jan;87(1):123–127. doi: 10.1073/pnas.87.1.123. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Rutter J. M., Luther P. D. Cell culture assay for toxigenic Pasteurella multocida from atrophic rhinitis of pigs. Vet Rec. 1984 Apr 21;114(16):393–396. doi: 10.1136/vr.114.16.393. [DOI] [PubMed] [Google Scholar]
  16. Rutter J. M., Mackenzie A. Pathogenesis of atrophic rhinitis in pigs: a new perspective. Vet Rec. 1984 Jan 28;114(4):89–90. doi: 10.1136/vr.114.4.89. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES