Abstract
The production and storage of explosives has resulted in the environmental accumulation of 2,4,6-trinitrotoluene (TNT). The biotransformation products of the nitroaromatic compound TNT and metabolites in mammalian cells in culture and their cytotoxicity are studied. We report after our analysis by reverse phase high performance liquid chromatography (HPLC) that the most prevalent biotransformation product of TNT in the NG108 neuroblastoma cells is primarily monoamino-dinitrotoluene (2Am-DNT). It causes toxic effects based on trypan blue exclusion and LDH-release colorimetric assays.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Brooks L. R., Jacobson R. W., Warren S. H., Kohan M. J., Donnelly K. C., George S. E. Mutagenicity of HPLC-fractionated urinary metabolites from 2,4,6-trinitrotoluene-treated Fischer 344 rats. Environ Mol Mutagen. 1997;30(3):298–302. [PubMed] [Google Scholar]
- Dutta S. K., Verma M. C-myc, c-fos, and c-ras coding sequences are conserved from yeast to mammals. Cancer Biochem Biophys. 1993 Jun;13(3):181–185. [PubMed] [Google Scholar]
- Rickert D. E., Butterworth B. E., Popp J. A. Dinitrotoluene: acute toxicity, oncogenicity, genotoxicity, and metabolism. Crit Rev Toxicol. 1984;13(3):217–234. doi: 10.3109/10408448409003373. [DOI] [PubMed] [Google Scholar]
- Sinensky M. C., Leiser A. L., Babich H. Oxidative stress aspects of the cytotoxicity of carbamide peroxide: in vitro studies. Toxicol Lett. 1995 Jan;75(1-3):101–109. doi: 10.1016/0378-4274(94)03168-7. [DOI] [PubMed] [Google Scholar]