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. 1991 May;59(5):1690–1696. doi: 10.1128/iai.59.5.1690-1696.1991

The alpha-L-(1----2)-trirhamnopyranoside epitope on the group-specific polysaccharide of group B streptococci.

F Michon 1, R Chalifour 1, R Feldman 1, M Wessels 1, D L Kasper 1, A Gamian 1, V Pozsgay 1, H J Jennings 1
PMCID: PMC257903  PMID: 1708356

Abstract

A number of epitope specificities associated with the group antigen (group B polysaccharide) of group B streptococci have been identified in a polyclonal antiserum induced in rabbits by a nonencapsulated variant strain of group B streptococci. This was achieved by using a series of oligosaccharide inhibitors, obtained by both synthetic and degradative procedures, to inhibit the binding of the group B polysaccharide to the polyclonal antiserum. While the dominant epitope expressed in the antiserum was alpha-L-Rhap(1----2)alpha-L-Rhap(1----2)alpha-L-Rhap, specificities associated with alpha-L-Rhap and alpha-L-Rhap(1----3)alpha-D-Galp(1----3)beta-D-Glcp-NAc(1----4)alp ha-L-Rhap were also identified. The dominant expression of the former epitope is consistent with its terminal location on the group antigen and also with highly branched multiantennary structure of this antigen. Antibodies specific for the alpha-L-trirhamnopyranoside epitope were purified by affinity chromatography, using the synthetic trisaccharide glucitol as the hapten. Oligosaccharide inhibition studies indicate that the specificity of these antibodies is identical to that of a murine monoclonal antibody induced by the same nonencapsulated strain of group B streptococci.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CURTIS S. N., KRAUSE R. M. ANTIGENIC RELATIONSHIPS BETWEEN GROUPS B AND G STREPTOCOCCI. J Exp Med. 1964 Oct 1;120:629–637. doi: 10.1084/jem.120.4.629. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Carey R. B., Eisenstein T. K., Shockman G. D., Greber T. F., Swenson R. M. Soluble group- and type-specific antigens from type III group B Streptococcus. Infect Immun. 1980 Apr;28(1):195–203. doi: 10.1128/iai.28.1.195-203.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Feldman R. G., Law S. M., Salisbury J. R. Detection of group B streptococcal antigen in necropsy specimens using monoclonal antibody and immunoperoxidase staining. J Clin Pathol. 1986 Feb;39(2):223–226. doi: 10.1136/jcp.39.2.223. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Heidelberger M., Davie J. M., Krause R. M. Cross-reactions of the group-specific polysaccharides of streptococcal groups B and G in anti-pneumococcal sera with especial reference to type 23 and its determinants. J Immunol. 1967 Oct;99(4):794–796. [PubMed] [Google Scholar]
  5. Jennings H. J., Lugowski C. Immunochemistry of groups A, B, and C meningococcal polysaccharide-tetanus toxoid conjugates. J Immunol. 1981 Sep;127(3):1011–1018. [PubMed] [Google Scholar]
  6. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  7. McCARTY M., LANCEFIELD R. C. Variation in the group-specific carbohydrate of group A streptococci. I. Immunochemical studies on the carbohydrates of variant strains. J Exp Med. 1955 Jul 1;102(1):11–28. doi: 10.1084/jem.102.1.11. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Michon F., Brisson J. R., Dell A., Kasper D. L., Jennings H. J. Multiantennary group-specific polysaccharide of group B Streptococcus. Biochemistry. 1988 Jul 12;27(14):5341–5351. doi: 10.1021/bi00414a059. [DOI] [PubMed] [Google Scholar]
  9. Michon F., Katzenellenbogen E., Kasper D. L., Jennings H. J. Structure of the complex group-specific polysaccharide of group B Streptococcus. Biochemistry. 1987 Jan 27;26(2):476–486. doi: 10.1021/bi00376a020. [DOI] [PubMed] [Google Scholar]
  10. Michon F., Pozsgay V., Brisson J. R., Jennings H. J. Substrate specificity of naringinase, an alpha-L-rhamnosidase from Penicillium decumbens. Carbohydr Res. 1989 Dec 1;194:321–324. doi: 10.1016/0008-6215(89)85033-5. [DOI] [PubMed] [Google Scholar]
  11. Pinto B. M., Bundle D. R. Preparation of glycoconjugates for use as artificial antigens: a simplified procedure. Carbohydr Res. 1983 Dec 23;124(2):313–318. doi: 10.1016/0008-6215(83)88466-3. [DOI] [PubMed] [Google Scholar]
  12. Porro M., Costantino P., Viti S., Vannozzi F., Naggi A., Torri G. Specific antibodies to diphtheria toxin and type 6A pneumococcal capsular polysaccharide induced by a model of semi-synthetic glycoconjugate antigen. Mol Immunol. 1985 Aug;22(8):907–919. doi: 10.1016/0161-5890(85)90077-x. [DOI] [PubMed] [Google Scholar]

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