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. 1991 May;59(5):1697–1702. doi: 10.1128/iai.59.5.1697-1702.1991

Interaction of Mycobacterium avium complex with human macrophages: roles of membrane receptors and serum proteins.

L E Bermudez 1, L S Young 1, H Enkel 1
PMCID: PMC257904  PMID: 1826895

Abstract

Invasive, disease-associated members of the Mycobacterium avium complex are facultative intracellular pathogens of mammalian macrophages. The mechanism(s) by which M. avium is ingested by mononuclear phagocytes is unknown. We examined the role of membrane receptors on macrophages as well as the role of opsonic components of the serum (complement, fibronectin, C-reactive protein and fibrinogen in the attachment and ingestion of M. avium by human monocyte-derived macrophages. Preincubation of serum with antibodies against C3 and fibronectin, in contrast to preincubation of serum with antibodies against complement-reactive protein and fibrinogen, significantly reduced the binding of M. avium to macrophages in concentration-dependent manner (57 to 93% and 35 to 61% inhibition by anti-C3 and anti-fibronectin antibody, respectively, in a concentration range of 25 to 100 micrograms/ml). We also observed that incubation of macrophages with OKM1 anti-complement receptor type 3 (CR3) antibody in the presence of serum decreased the binding of M. avium to macrophages by 86% +/- 6%, while the same antibody inhibited binding by 63% +/- 7% in the absence of serum. In contrast, incubation of macrophages with anti-LFA-1, anti-p 150.95, anti-CR1, or anti-Mac-1 had no effect on the ability of M. avium to bind to the cell. In addition, incubation of macrophages with alpha-methyl-D-mannoside was also associated with decreased attachment of M. avium to macrophages. These results provide evidence for the role of three macrophage receptors (CR3, fibronectin, and mannosyl-fucosyl receptors) in the uptake of M. avium by human macrophages.

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Selected References

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  1. Abou-Zeid C., Ratliff T. L., Wiker H. G., Harboe M., Bennedsen J., Rook G. A. Characterization of fibronectin-binding antigens released by Mycobacterium tuberculosis and Mycobacterium bovis BCG. Infect Immun. 1988 Dec;56(12):3046–3051. doi: 10.1128/iai.56.12.3046-3051.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Aslanzadeh J., Brown E. J., Quillin S. P., Ritchey J. K., Ratliff T. L. Characterization of soluble fibronectin binding to Bacille Calmette-Guérin. J Gen Microbiol. 1989 Oct;135(10):2735–2741. doi: 10.1099/00221287-135-10-2735. [DOI] [PubMed] [Google Scholar]
  3. Baughn R. E. Antibody-independent interactions of fibronectin, C1q, and human neutrophils with Treponema pallidum. Infect Immun. 1986 Nov;54(2):456–464. doi: 10.1128/iai.54.2.456-464.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bermudez L. E., Young L. S. Phagocytosis and intracellular killing of Mycobacterium avium complex by human and murine macrophages. Braz J Med Biol Res. 1987;20(2):191–201. [PubMed] [Google Scholar]
  5. Bermudez L. E., Young L. S. Tumor necrosis factor, alone or in combination with IL-2, but not IFN-gamma, is associated with macrophage killing of Mycobacterium avium complex. J Immunol. 1988 May 1;140(9):3006–3013. [PubMed] [Google Scholar]
  6. Bertram M. A., Inderlied C. B., Yadegar S., Kolanoski P., Yamada J. K., Young L. S. Confirmation of the beige mouse model for study of disseminated infection with Mycobacterium avium complex. J Infect Dis. 1986 Jul;154(1):194–195. doi: 10.1093/infdis/154.1.194. [DOI] [PubMed] [Google Scholar]
  7. Bullock W. E., Wright S. D. Role of the adherence-promoting receptors, CR3, LFA-1, and p150,95, in binding of Histoplasma capsulatum by human macrophages. J Exp Med. 1987 Jan 1;165(1):195–210. doi: 10.1084/jem.165.1.195. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. CUMMINS C. S. Chemical composition and antigenic structure of cell walls of Corynebacterium, Mycobacterium, Nocardia, Actinomyces and Arthrobacter. J Gen Microbiol. 1962 Apr;28:35–50. doi: 10.1099/00221287-28-1-35. [DOI] [PubMed] [Google Scholar]
  9. Calderone R. A., Linehan L., Wadsworth E., Sandberg A. L. Identification of C3d receptors on Candida albicans. Infect Immun. 1988 Jan;56(1):252–258. doi: 10.1128/iai.56.1.252-258.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Chang C. S., Chang K. P. Monoclonal antibody affinity purification of a Leishmania membrane glycoprotein and its inhibition of leishmania-macrophage binding. Proc Natl Acad Sci U S A. 1986 Jan;83(1):100–104. doi: 10.1073/pnas.83.1.100. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Cosio F. G. Human fibronectin is an opsonin for IgG antibody-coated sheep erythrocytes. J Lab Clin Med. 1984 Apr;103(4):613–619. [PubMed] [Google Scholar]
  12. Crowle A. J., Tsang A. Y., Vatter A. E., May M. H. Comparison of 15 laboratory and patient-derived strains of Mycobacterium avium for ability to infect and multiply in cultured human macrophages. J Clin Microbiol. 1986 Nov;24(5):812–821. doi: 10.1128/jcm.24.5.812-821.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hawkins C. C., Gold J. W., Whimbey E., Kiehn T. E., Brannon P., Cammarata R., Brown A. E., Armstrong D. Mycobacterium avium complex infections in patients with the acquired immunodeficiency syndrome. Ann Intern Med. 1986 Aug;105(2):184–188. doi: 10.7326/0003-4819-105-2-184. [DOI] [PubMed] [Google Scholar]
  14. Kishimoto T. K., Larson R. S., Corbi A. L., Dustin M. L., Staunton D. E., Springer T. A. The leukocyte integrins. Adv Immunol. 1989;46:149–182. doi: 10.1016/s0065-2776(08)60653-7. [DOI] [PubMed] [Google Scholar]
  15. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  16. Mosser D. M., Edelson P. J. The mouse macrophage receptor for C3bi (CR3) is a major mechanism in the phagocytosis of Leishmania promastigotes. J Immunol. 1985 Oct;135(4):2785–2789. [PubMed] [Google Scholar]
  17. Nakagawara A., Nathan C. F. A simple method for counting adherent cells: application to cultured human monocytes, macrophages and multinucleated giant cells. J Immunol Methods. 1983 Jan 28;56(2):261–268. doi: 10.1016/0022-1759(83)90418-0. [DOI] [PubMed] [Google Scholar]
  18. Schlesinger L. S., Bellinger-Kawahara C. G., Payne N. R., Horwitz M. A. Phagocytosis of Mycobacterium tuberculosis is mediated by human monocyte complement receptors and complement component C3. J Immunol. 1990 Apr 1;144(7):2771–2780. [PubMed] [Google Scholar]
  19. Schlesinger L. S., Horwitz M. A. Phagocytosis of leprosy bacilli is mediated by complement receptors CR1 and CR3 on human monocytes and complement component C3 in serum. J Clin Invest. 1990 Apr;85(4):1304–1314. doi: 10.1172/JCI114568. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Simpson W. A., Hasty D. L., Mason J. M., Beachey E. H. Fibronectin-mediated binding of group A streptococci to human polymorphonuclear leukocytes. Infect Immun. 1982 Aug;37(2):805–810. doi: 10.1128/iai.37.2.805-810.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Swartz R. P., Naai D., Vogel C. W., Yeager H., Jr Differences in uptake of mycobacteria by human monocytes: a role for complement. Infect Immun. 1988 Sep;56(9):2223–2227. doi: 10.1128/iai.56.9.2223-2227.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Wilson M. E., Pearson R. D. Roles of CR3 and mannose receptors in the attachment and ingestion of Leishmania donovani by human mononuclear phagocytes. Infect Immun. 1988 Feb;56(2):363–369. doi: 10.1128/iai.56.2.363-369.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Wright S. D., Silverstein S. C. Receptors for C3b and C3bi promote phagocytosis but not the release of toxic oxygen from human phagocytes. J Exp Med. 1983 Dec 1;158(6):2016–2023. doi: 10.1084/jem.158.6.2016. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Young L. S., Inderlied C. B., Berlin O. G., Gottlieb M. S. Mycobacterial infections in AIDS patients, with an emphasis on the Mycobacterium avium complex. Rev Infect Dis. 1986 Nov-Dec;8(6):1024–1033. doi: 10.1093/clinids/8.6.1024. [DOI] [PubMed] [Google Scholar]

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