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. 1991 Sep;59(9):3313–3315. doi: 10.1128/iai.59.9.3313-3315.1991

Identification of subregions of Bordetella pertussis filamentous hemagglutinin that stimulate human T-cell responses.

A Di Tommaso 1, M Domenighini 1, M Bugnoli 1, A Tagliabue 1, R Rappuoli 1, M T De Magistris 1
PMCID: PMC258172  PMID: 1715327

Abstract

Filamentous hemagglutinin (FHA), a 220-kDa protein that mediates the adhesion of Bordetella pertussis to eukaryotic cells, is a component of acellular vaccines against whooping cough. To identify the subregions of FHA that are immunogenic for T cells, 16 human T-cell clones were raised against purified FHA and tested for the recognition of recombinant and proteolytic fragments. The clones were found to map either in the carboxy-terminal or the amino-terminal part of the FHA molecule, but none of them recognized the central region, which contains a sequence that is homologous to that of the eukaryotic protein fibronectin. These data suggest that subregions of FHA that do not contain sequences that are potentially cross-reactive with self proteins may be sufficient to induce an immune response against the whole protein.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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