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. 1990 Jun;58(6):1614–1619. doi: 10.1128/iai.58.6.1614-1619.1990

Identification of a bacteriophage containing a silent staphylococcal variant enterotoxin gene (sezA+).

M T Soltis 1, J J Mekalanos 1, M J Betley 1
PMCID: PMC258687  PMID: 2140340

Abstract

A variant enterotoxin gene, referred to as sezA+, has been identified. Staphylococcus aureus FRI1106, a staphylococcal enterotoxin type D producer (Sed+), contained HindIII fragments of 3.8 and 9.4 kilobase pairs (kbp) that hybridized in Southern blot analysis to a probe containing only staphylococcal enterotoxin type A structural gene sequences. Presumably, probe A-624 hybridized to the 9.4-kbp HindIII fragment because of the sequence homology between sea+ and sed+. This 9.4-kbp HindIII fragment, which was part of a staphylococcal plasmid, was isolated and ligated into an Escherichia coli plasmid vector; Sed+ E. coli recombinant clones were isolated. The 3.8-kbp HindIII fragment was shown to be part of a viable lysogenic bacteriophage, and it contained sezA+. This sezA(+)-containing fragment was cloned into E. coli, and its DNA sequence was determined. Examination of the nucleotide sequence revealed a 771-bp region that contained an open reading frame with 85 and 77% nucleotide and derived amino acid sequence identifies with sea+ and staphylococcal enterotoxin type A, respectively. This open reading frame has 83 to 50% nucleotide sequence identities with the other types of staphylococcal enterotoxin genes. sezA+ was shown to be transcribed into stable mRNA. However, the sezA+ mRNA was not translated into an enterotoxinlike protein because it lacks an appropriate translation initiation codon.

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Selected References

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  1. Altboum Z., Hertman I., Sarid S. Penicillinase plasmid-linked genetic determinants for enterotoxins B and C1 production in Staphylococcus aureus. Infect Immun. 1985 Feb;47(2):514–521. doi: 10.1128/iai.47.2.514-521.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bayles K. W., Iandolo J. J. Genetic and molecular analyses of the gene encoding staphylococcal enterotoxin D. J Bacteriol. 1989 Sep;171(9):4799–4806. doi: 10.1128/jb.171.9.4799-4806.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Betley M. J., Löfdahl S., Kreiswirth B. N., Bergdoll M. S., Novick R. P. Staphylococcal enterotoxin A gene is associated with a variable genetic element. Proc Natl Acad Sci U S A. 1984 Aug;81(16):5179–5183. doi: 10.1073/pnas.81.16.5179. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Betley M. J., Mekalanos J. J. Nucleotide sequence of the type A staphylococcal enterotoxin gene. J Bacteriol. 1988 Jan;170(1):34–41. doi: 10.1128/jb.170.1.34-41.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Betley M. J., Mekalanos J. J. Staphylococcal enterotoxin A is encoded by phage. Science. 1985 Jul 12;229(4709):185–187. doi: 10.1126/science.3160112. [DOI] [PubMed] [Google Scholar]
  6. Bohach G. A., Schlievert P. M. Conservation of the biologically active portions of staphylococcal enterotoxins C1 and C2. Infect Immun. 1989 Jul;57(7):2249–2252. doi: 10.1128/iai.57.7.2249-2252.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Bohach G. A., Schlievert P. M. Nucleotide sequence of the staphylococcal enterotoxin C1 gene and relatedness to other pyrogenic toxins. Mol Gen Genet. 1987 Aug;209(1):15–20. doi: 10.1007/BF00329830. [DOI] [PubMed] [Google Scholar]
  8. Bolivar F., Rodriguez R. L., Greene P. J., Betlach M. C., Heyneker H. L., Boyer H. W., Crosa J. H., Falkow S. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene. 1977;2(2):95–113. [PubMed] [Google Scholar]
  9. Couch J. L., Betley M. J. Nucleotide sequence of the type C3 staphylococcal enterotoxin gene suggests that intergenic recombination causes antigenic variation. J Bacteriol. 1989 Aug;171(8):4507–4510. doi: 10.1128/jb.171.8.4507-4510.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Couch J. L., Soltis M. T., Betley M. J. Cloning and nucleotide sequence of the type E staphylococcal enterotoxin gene. J Bacteriol. 1988 Jul;170(7):2954–2960. doi: 10.1128/jb.170.7.2954-2960.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Crass B. A., Bergdoll M. S. Toxin involvement in toxic shock syndrome. J Infect Dis. 1986 May;153(5):918–926. doi: 10.1093/infdis/153.5.918. [DOI] [PubMed] [Google Scholar]
  12. Devereux J., Haeberli P., Smithies O. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387–395. doi: 10.1093/nar/12.1part1.387. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Edwin C., Tatini S. R., Strobel R. S., Maheswaran S. K. Production of monoclonal antibodies to staphylococcal enterotoxin A. Appl Environ Microbiol. 1984 Dec;48(6):1171–1175. doi: 10.1128/aem.48.6.1171-1175.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Fast D. J., Schlievert P. M., Nelson R. D. Toxic shock syndrome-associated staphylococcal and streptococcal pyrogenic toxins are potent inducers of tumor necrosis factor production. Infect Immun. 1989 Jan;57(1):291–294. doi: 10.1128/iai.57.1.291-294.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Garbe P. L., Arko R. J., Reingold A. L., Graves L. M., Hayes P. S., Hightower A. W., Chandler F. W., Broome C. V. Staphylococcus aureus isolates from patients with nonmenstrual toxic shock syndrome. Evidence for additional toxins. JAMA. 1985 May 3;253(17):2538–2542. [PubMed] [Google Scholar]
  16. Gold L. Posttranscriptional regulatory mechanisms in Escherichia coli. Annu Rev Biochem. 1988;57:199–233. doi: 10.1146/annurev.bi.57.070188.001215. [DOI] [PubMed] [Google Scholar]
  17. Gold L., Pribnow D., Schneider T., Shinedling S., Singer B. S., Stormo G. Translational initiation in prokaryotes. Annu Rev Microbiol. 1981;35:365–403. doi: 10.1146/annurev.mi.35.100181.002053. [DOI] [PubMed] [Google Scholar]
  18. Goshorn S. C., Schlievert P. M. Nucleotide sequence of streptococcal pyrogenic exotoxin type C. Infect Immun. 1988 Sep;56(9):2518–2520. doi: 10.1128/iai.56.9.2518-2520.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Gren E. J. Recognition of messenger RNA during translational initiation in Escherichia coli. Biochimie. 1984 Jan;66(1):1–29. doi: 10.1016/0300-9084(84)90188-3. [DOI] [PubMed] [Google Scholar]
  20. Huang I. Y., Bergdoll M. S. The primary structure of staphylococcal enterotoxin B. 3. The cyanogen bromide peptides of reduced and aminoethylated enterotoxin B, and the complete amino acid sequence. J Biol Chem. 1970 Jul 25;245(14):3518–3525. [PubMed] [Google Scholar]
  21. Huang I. Y., Hughes J. L., Bergdoll M. S., Schantz E. J. Complete amino acid sequence of staphylococcal enterotoxin A. J Biol Chem. 1987 May 25;262(15):7006–7013. [PubMed] [Google Scholar]
  22. Janeway C. A., Jr, Yagi J., Conrad P. J., Katz M. E., Jones B., Vroegop S., Buxser S. T-cell responses to Mls and to bacterial proteins that mimic its behavior. Immunol Rev. 1989 Feb;107:61–88. doi: 10.1111/j.1600-065x.1989.tb00003.x. [DOI] [PubMed] [Google Scholar]
  23. Johns M. B., Jr, Khan S. A. Staphylococcal enterotoxin B gene is associated with a discrete genetic element. J Bacteriol. 1988 Sep;170(9):4033–4039. doi: 10.1128/jb.170.9.4033-4039.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Jones C. L., Khan S. A. Nucleotide sequence of the enterotoxin B gene from Staphylococcus aureus. J Bacteriol. 1986 Apr;166(1):29–33. doi: 10.1128/jb.166.1.29-33.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Lee A. C., Robbins R. N., Bergdoll M. S. Isolation of specific and common antibodies to staphylococcal enterotoxins A and E by affinity chromatography. Infect Immun. 1978 Aug;21(2):387–391. doi: 10.1128/iai.21.2.387-391.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Lipman D. J., Pearson W. R. Rapid and sensitive protein similarity searches. Science. 1985 Mar 22;227(4693):1435–1441. doi: 10.1126/science.2983426. [DOI] [PubMed] [Google Scholar]
  27. McLaughlin J. R., Murray C. L., Rabinowitz J. C. Unique features in the ribosome binding site sequence of the gram-positive Staphylococcus aureus beta-lactamase gene. J Biol Chem. 1981 Nov 10;256(21):11283–11291. [PubMed] [Google Scholar]
  28. Norrander J., Kempe T., Messing J. Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis. Gene. 1983 Dec;26(1):101–106. doi: 10.1016/0378-1119(83)90040-9. [DOI] [PubMed] [Google Scholar]
  29. Novick R. P., Brodsky R. Studies on plasmid replication. I. Plasmid incompatibility and establishment in Staphylococcus aureus. J Mol Biol. 1972 Jul 21;68(2):285–302. doi: 10.1016/0022-2836(72)90214-8. [DOI] [PubMed] [Google Scholar]
  30. Novick R. P., Murphy E., Gryczan T. J., Baron E., Edelman I. Penicillinase plasmids of Staphylococcus aureus: restriction-deletion maps. Plasmid. 1979 Jan;2(1):109–129. doi: 10.1016/0147-619x(79)90010-6. [DOI] [PubMed] [Google Scholar]
  31. Parsonnet J., Gillis Z. A., Pier G. B. Induction of interleukin-1 by strains of Staphylococcus aureus from patients with nonmenstrual toxic shock syndrome. J Infect Dis. 1986 Jul;154(1):55–63. doi: 10.1093/infdis/154.1.55. [DOI] [PubMed] [Google Scholar]
  32. Robbins R., Gould S., Bergdoll M. Detecting the enterotoxigenicity of Staphylococcus aureus strains. Appl Microbiol. 1974 Dec;28(6):946–950. doi: 10.1128/am.28.6.946-950.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Sandler P., Weisblum B. Erythromycin-induced stabilization of ermA messenger RNA in Staphylococcus aureus and Bacillus subtilis. J Mol Biol. 1988 Oct 20;203(4):905–915. doi: 10.1016/0022-2836(88)90116-7. [DOI] [PubMed] [Google Scholar]
  34. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Schlievert P. M. Staphylococcal enterotoxin B and toxic-shock syndrome toxin-1 are significantly associated with non-menstrual TSS. Lancet. 1986 May 17;1(8490):1149–1150. doi: 10.1016/s0140-6736(86)91859-3. [DOI] [PubMed] [Google Scholar]
  36. Schmidt J. J., Spero L. The complete amino acid sequence of staphylococcal enterotoxin C1. J Biol Chem. 1983 May 25;258(10):6300–6306. [PubMed] [Google Scholar]
  37. Stobberingh E. E., Schiphof R., Sussenbach J. S. Occurrence of a class II restriction endonuclease in Staphylococcus aureus. J Bacteriol. 1977 Aug;131(2):645–649. doi: 10.1128/jb.131.2.645-649.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  38. Thompson N. E., Razdan M., Kuntsmann G., Aschenbach J. M., Evenson M. L., Bergdoll M. S. Detection of staphylococcal enterotoxins by enzyme-linked immunosorbent assays and radioimmunoassays: comparison of monoclonal and polyclonal antibody systems. Appl Environ Microbiol. 1986 May;51(5):885–890. doi: 10.1128/aem.51.5.885-890.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  39. Weeks C. R., Ferretti J. J. Nucleotide sequence of the type A streptococcal exotoxin (erythrogenic toxin) gene from Streptococcus pyogenes bacteriophage T12. Infect Immun. 1986 Apr;52(1):144–150. doi: 10.1128/iai.52.1.144-150.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]

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