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. 1990 Jun;58(6):1909–1913. doi: 10.1128/iai.58.6.1909-1913.1990

Expression in Escherichia coli of a high-molecular-weight protective surface antigen found in nontypeable and type b Haemophilus influenzae.

W R Thomas 1, M G Callow 1, R J Dilworth 1, A A Audesho 1
PMCID: PMC258742  PMID: 2187812

Abstract

An Escherichia coli clone producing a high-molecular-weight surface antigen of Haemophilus influenzae type b (Hib) was isolated from a library of Hib DNA fragments cloned as lysogens in a lambda replacement vector. The antigen is found in sarcosyl-insoluble outer membrane protein preparations and was produced by all 36 H. influenzae isolates tested. Absorption studies indicated that the antigen is a surface determinant on all isolates tested. Antibodies to the antigen (D15) were found in eight of nine convalescent-phase sera from children with invasive Hib infection. Affinity-purified antibodies prepared against the cloned antigen gave protection against the development of bacteremia in a rat pup model.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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