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. 1990 Jun;58(6):1986–1991. doi: 10.1128/iai.58.6.1986-1991.1990

Binding of colloidal gold-labeled salivary proline-rich proteins to Actinomyces viscosus type 1 fimbriae.

K P Leung 1, W E Nesbitt 1, W Fischlschweiger 1, D I Hay 1, W B Clark 1
PMCID: PMC258754  PMID: 1971260

Abstract

Salivary proline-rich proteins (PRPs), which were purified from parotid saliva, were adsorbed onto 15-nm-diameter gold particles to visualize specific binding of the salivary molecules to Actinomyces viscosus type 1 fimbriae. Negatively stained preparations incubated with PRP-gold conjugates but not bovine serum albumin-gold complexes bound specifically to bacteria possessing type 1 fimbriae, A. viscosus T14V-J1 and 5519. Binding of the PRP-gold probes to strains deficient in type 1 fimbriae, i.e., strains 5951 (type 2 fimbriae only) and 147 (no fimbriae), was negligible.

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1987

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Selected References

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