Skip to main content
Infection and Immunity logoLink to Infection and Immunity
. 1990 Aug;58(8):2706–2709. doi: 10.1128/iai.58.8.2706-2709.1990

The cytolysin gene of Vibrio vulnificus: sequence and relationship to the Vibrio cholerae E1 Tor hemolysin gene.

K Yamamoto 1, A C Wright 1, J B Kaper 1, J G Morris Jr 1
PMCID: PMC258880  PMID: 2370116

Abstract

A cytolysin of ca. 56 kilodaltons has been suggested as a possible virulence factor in Vibrio vulnificus infections. We sequenced the DNA encoding cytolytic activity and found that the sequence contained two open reading frames, vvhA and vvhB. vvhA encoded the structural gene for the cytolysin and contained the N-terminal amino acid sequence previously reported for the protein. Regions of the vvhA gene showed homology to the structural gene for the Vibrio cholerae E1 Tor hemolysin.

Full text

PDF
2709

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alm R. A., Stroeher U. H., Manning P. A. Extracellular proteins of Vibrio cholerae: nucleotide sequence of the structural gene (hlyA) for the haemolysin of the haemolytic El Tor strain 017 and characterization of the hlyA mutation in the non-haemolytic classical strain 569B. Mol Microbiol. 1988 Jul;2(4):481–488. doi: 10.1111/j.1365-2958.1988.tb00054.x. [DOI] [PubMed] [Google Scholar]
  2. Blake P. A., Merson M. H., Weaver R. E., Hollis D. G., Heublein P. C. Disease caused by a marine Vibrio. Clinical characteristics and epidemiology. N Engl J Med. 1979 Jan 4;300(1):1–5. doi: 10.1056/NEJM197901043000101. [DOI] [PubMed] [Google Scholar]
  3. Devereux J., Haeberli P., Smithies O. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387–395. doi: 10.1093/nar/12.1part1.387. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gray L. D., Kreger A. S. Purification and characterization of an extracellular cytolysin produced by Vibrio vulnificus. Infect Immun. 1985 Apr;48(1):62–72. doi: 10.1128/iai.48.1.62-72.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hong G. F. A systemic DNA sequencing strategy. J Mol Biol. 1982 Jul 5;158(3):539–549. doi: 10.1016/0022-2836(82)90213-3. [DOI] [PubMed] [Google Scholar]
  6. Klontz K. C., Lieb S., Schreiber M., Janowski H. T., Baldy L. M., Gunn R. A. Syndromes of Vibrio vulnificus infections. Clinical and epidemiologic features in Florida cases, 1981-1987. Ann Intern Med. 1988 Aug 15;109(4):318–323. doi: 10.7326/0003-4819-109-4-318. [DOI] [PubMed] [Google Scholar]
  7. Kreger A., Lockwood D. Detection of extracellular toxin(s) produced by Vibrio vulnificus. Infect Immun. 1981 Aug;33(2):583–590. doi: 10.1128/iai.33.2.583-590.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Messing J., Crea R., Seeburg P. H. A system for shotgun DNA sequencing. Nucleic Acids Res. 1981 Jan 24;9(2):309–321. doi: 10.1093/nar/9.2.309. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Morris J. G., Jr, Black R. E. Cholera and other vibrioses in the United States. N Engl J Med. 1985 Feb 7;312(6):343–350. doi: 10.1056/NEJM198502073120604. [DOI] [PubMed] [Google Scholar]
  10. Morris J. G., Jr, Wright A. C., Roberts D. M., Wood P. K., Simpson L. M., Oliver J. D. Identification of environmental Vibrio vulnificus isolates with a DNA probe for the cytotoxin-hemolysin gene. Appl Environ Microbiol. 1987 Jan;53(1):193–195. doi: 10.1128/aem.53.1.193-195.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Nishibuchi M., Khaeomanee-iam V., Honda T., Kaper J. B., Miwatani T. Comparative analysis of the hemolysin genes of Vibrio cholerae non-01, V. mimicus, and V. hollisae that are similar to the tdh gene of V. parahaemolyticus. FEMS Microbiol Lett. 1990 Feb;55(3):251–256. doi: 10.1016/0378-1097(90)90004-a. [DOI] [PubMed] [Google Scholar]
  12. Okada K., Miake S., Moriya T., Mitsuyama M., Amako K. Variability of haemolysin(s) produced by Vibrio vulnificus. J Gen Microbiol. 1987 Oct;133(10):2853–2857. doi: 10.1099/00221287-133-10-2853. [DOI] [PubMed] [Google Scholar]
  13. Pollak S. J., Parrish E. F., 3rd, Barrett T. J., Dretler R., Morris J. G., Jr Vibrio vulnificus septicemia. Isolation of organism from stool and demonstration of antibodies by indirect immunofluorescence. Arch Intern Med. 1983 Apr;143(4):837–838. doi: 10.1001/archinte.143.4.837. [DOI] [PubMed] [Google Scholar]
  14. Rader A. E., Murphy J. R. Nucleotide sequences and comparison of the hemolysin determinants of Vibrio cholerae El Tor RV79(Hly+) and RV79(Hly-) and classical 569B(Hly-). Infect Immun. 1988 Jun;56(6):1414–1419. doi: 10.1128/iai.56.6.1414-1419.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Testa J., Daniel L. W., Kreger A. S. Extracellular phospholipase A2 and lysophospholipase produced by Vibrio vulnificus. Infect Immun. 1984 Aug;45(2):458–463. doi: 10.1128/iai.45.2.458-463.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Wright A. C., Morris J. G., Jr, Maneval D. R., Jr, Richardson K., Kaper J. B. Cloning of the cytotoxin-hemolysin gene of Vibrio vulnificus. Infect Immun. 1985 Dec;50(3):922–924. doi: 10.1128/iai.50.3.922-924.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Yamamoto K., Al-Omani M., Honda T., Takeda Y., Miwatani T. Non-O1 Vibrio cholerae hemolysin: purification, partial characterization, and immunological relatedness to El Tor hemolysin. Infect Immun. 1984 Jul;45(1):192–196. doi: 10.1128/iai.45.1.192-196.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES