Abstract
The objective of this study was to develop a rapid in vitro method for predicting the response of human tumors to anticancer drugs. In this study an attempt was made to correlate the drug effects on the relative incorporation of (3H) thymidine (ThdR) into DNA with the sensitivity of tumor cells to that drug. The results of the study indicate that following treatment of the cells with adriamycin (ADR) or 1-(2-chloroethyl)-3-(4-methyl cyclohexyl)-1 nitrosourea (MeCCNU), there was a significant inhibition of DNA synthesis in the drug-sensitive cells. However, the inhibition was relatively small in the drug-resistant cells. Following cytosine arabinoside (Ara-C) treatment, a dramatic recovery in the rate of DNA synthesis was seen in Ara-C-resistant cells but not in cells sensitive to Ara-C. Thus, the method described in this study appears to be capable of distinguishing whether a tumor cell line is sensitive or resistant to a given drug.
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Selected References
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- Bech-Hansen N. T., Sarangi F., Sutherland D. J., Ling V. Rapid assays for evaluating the drug sensitivity of tumor cells. J Natl Cancer Inst. 1977 Jul;59(1):21–27. doi: 10.1093/jnci/59.1.21. [DOI] [PubMed] [Google Scholar]
- Erickson L. C., Osieka R., Kohn K. W. Differential repair of 1-(2-chloroethyl)-3-(4-methylcyclohexyl)-1-nitrosourea-induced DNA damage in two human colon tumor cell lines. Cancer Res. 1978 Mar;38(3):802–808. [PubMed] [Google Scholar]
- Foster D. A. 1-beta-D-arabinofuranosylcytosine stimulates thymidine incorporation into the DNA of contact-inhibited cells. Exp Cell Res. 1981 Oct;135(2):427–431. doi: 10.1016/0014-4827(81)90183-x. [DOI] [PubMed] [Google Scholar]
- Inaba M., Kobayashi H., Sakurai Y., Johnson R. K. Active efflux of daunorubicin and adriamycin in sensitive and resistant sublines of P388 leukemia. Cancer Res. 1979 Jun;39(6 Pt 1):2200–2203. [PubMed] [Google Scholar]
- Inaba M., Sakurai Y. Enhanced efflux of actinomycin D, vincristine, and vinblastine in adriamycin-resistant subline of P388 leukemia. Cancer Lett. 1979 Dec;8(2):111–115. doi: 10.1016/0304-3835(79)90003-x. [DOI] [PubMed] [Google Scholar]
- Painter R. B. DNA synthesis inhibition in HeLa cells as a simple test for agents that damage human DNA. J Environ Pathol Toxicol. 1978 Sep-Oct;2(1):65–78. [PubMed] [Google Scholar]
- Painter R. B., Howard R. The Hela DNA-synthesis inhibition test as a rapid screen for mutagenic carcinogens. Mutat Res. 1982 Feb 22;92(1-2):427–437. doi: 10.1016/0027-5107(82)90241-x. [DOI] [PubMed] [Google Scholar]
- Painter R. B. Rapid test to detect agents that damage human DNA. Nature. 1977 Feb 17;265(5595):650–651. doi: 10.1038/265650a0. [DOI] [PubMed] [Google Scholar]
- Salmon S. E., Hamburger A. W., Soehnlen B., Durie B. G., Alberts D. S., Moon T. E. Quantitation of differential sensitivity of human-tumor stem cells to anticancer drugs. N Engl J Med. 1978 Jun 15;298(24):1321–1327. doi: 10.1056/NEJM197806152982401. [DOI] [PubMed] [Google Scholar]
- Volm M., Wayss K., Kaufmann M., Mattern J. Pretherapeutic detection of tumour resistance and the results of tumour chemotherapy. Eur J Cancer. 1979 Jul;15(7):983–993. doi: 10.1016/0014-2964(79)90282-2. [DOI] [PubMed] [Google Scholar]
- Wilkoff L. J., Dulmadge E. A. Resistance and cross-resistance of cultured leukemia P388 cells to vincristine, adriamycin, adriamycin analogs, and actinomycin D. J Natl Cancer Inst. 1978 Dec;61(6):1521–1524. [PubMed] [Google Scholar]
- Woodcock D. M., Cooper I. A. Aberrant double replication of segments of chromosomal DNA following DNA synthesis inhibition by cytosine arabinoside. Exp Cell Res. 1979 Oct 1;123(1):157–166. doi: 10.1016/0014-4827(79)90432-4. [DOI] [PubMed] [Google Scholar]