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. 1991 Nov;59(11):4142–4146. doi: 10.1128/iai.59.11.4142-4146.1991

A new in vitro model of Entamoeba histolytica adhesion, using the human colon carcinoma cell line Caco-2: scanning electron microscopic study.

M C Rigothier 1, M H Coconnier 1, A L Servin 1, P Gayral 1
PMCID: PMC259008  PMID: 1937772

Abstract

The human colon carcinoma cell line Caco-2, which is widely used to study the adhesion and cytotoxicity of enterobacteria, was used to investigate the adhesion of the trophozoites of Entamoeba histolytica. We observed a high percentage of adhesion of amoebae to Caco-2 cells. Scanning electron microscopy showed that amoebial membrane structures were involved in adhesion and the cytolytic action. These differentiated cells should prove to be a useful model system for investigation of the pathogenic action of amoebae.

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Selected References

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