Abstract
Spiroplasma strains of group IV were compared by two-dimensional protein analyses on polyacrylamide gels. Although considerable diversity was evident, the assemblages studied were less heterogeneous than the known strains of group I. Two electrophoretic techniques were used to identify spiroplasma proteins that had been used to immunize rabbits. These included monoclonal antibodies prepared against Spiroplasma citri. In the first technique, protein antigens were purified by immunoaffinity chromatography, then identified with SDS-PAGE. In the second technique, spiroplasma proteins were first separated by SDS-PAGE, then antigens were identified by antibody binding to blot-transferred proteins. Finally, two-dimensional protein electrophoresis has been used as a source of immunogens to characterize monospecific antibodies against individual S. citri proteins.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bové J. M., Mouches C., Carle-Junca P., Degorce-Dumas J. R., Tully J. G., Whitcomb R. F. Spiroplasmas of Group I: the Spiroplasma citri cluster. Yale J Biol Med. 1983 Sep-Dec;56(5-6):573–582. [PMC free article] [PubMed] [Google Scholar]
- Bowen B., Steinberg J., Laemmli U. K., Weintraub H. The detection of DNA-binding proteins by protein blotting. Nucleic Acids Res. 1980 Jan 11;8(1):1–20. doi: 10.1093/nar/8.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Buck D. W., Kennett R. H., McGarrity G. Monoclonal antibodies specific for cell culture mycoplasmas. In Vitro. 1982 Apr;18(4):377–381. doi: 10.1007/BF02796338. [DOI] [PubMed] [Google Scholar]
- Wroblewski H., Burlot R., Johansson K. E. Solubilization of Spiroplasma citri cell membrane proteins with the anionic detergent sodium lauroyl-sarcosinate (Sarkosyl). Biochimie. 1978;60(4):389–398. doi: 10.1016/s0300-9084(78)80672-5. [DOI] [PubMed] [Google Scholar]