Skip to main content
PMC home page
The Yale Journal of Biology and Medicine logoLink to The Yale Journal of Biology and Medicine
. 1983 Sep-Dec;56(5-6):709–716.

The metabolic pathways of Acholeplasma and Mycoplasma: an overview.

J D Pollack, V V Tryon, K D Beaman
PMCID: PMC2590559  PMID: 6206660

Abstract

The metabolism of the Mollicutes Acholeplasma and Mycoplasma may be characterized as restricted, for example, by virtue of the apparent absence of cytochrome pigments. Some Mollicutes have lowered ECA values during their logarithmic growth phase, which we speculate may be related to insufficient substrate phosphorylation or insufficient ATP synthesis linked to glycolysis. We found that PEP is carboxylated by preparations of A. laidlawii, but not by other Mollicutes; thus in this organism oxaloacetate from PEP may be a link to other pathways. We found phosphoribosylpyrophosphate in A. laidlawii, which suggests that ribosylation of purines and pyrimidines occurs in Mollicutes other than M. mycoides.

Full text

PDF
709

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beaman K. D., Pollack J. D. Adenylate energy charge in Acholeplasma laidlawii. J Bacteriol. 1981 Jun;146(3):1055–1058. doi: 10.1128/jb.146.3.1055-1058.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. CASTREJON-DIEZ J., FISHER T. N., FISHER E., Jr GLUCOSE METABOLISM OF TWO STRAINS OF MYCOPLASMA LAIDLAWII. J Bacteriol. 1963 Oct;86:627–636. doi: 10.1128/jb.86.4.627-636.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chapman A. G., Atkinson D. E. Adenine nucleotide concentrations and turnover rates. Their correlation with biological activity in bacteria and yeast. Adv Microb Physiol. 1977;15:253–306. doi: 10.1016/s0065-2911(08)60318-5. [DOI] [PubMed] [Google Scholar]
  4. Fenske J. D., Kenny G. E. Role of arginine deiminase in growth of Mycoplasma hominis. J Bacteriol. 1976 Apr;126(1):501–510. doi: 10.1128/jb.126.1.501-510.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gabridge M. G., Stahl Y. D. Role of adenine in pathogenesis of Mycoplasma pneumoniae infections of tracheal epithelium. Med Microbiol Immunol. 1978 May 26;165(1):43–55. doi: 10.1007/BF02121231. [DOI] [PubMed] [Google Scholar]
  6. Hamet M., Bonissol C., Cartier P. Enzymatic activities on purine pyrimidine metabolism in nine mycoplasma species contaminating cell cultures. Clin Chim Acta. 1980 Apr 11;103(1):15–22. doi: 10.1016/0009-8981(80)90225-9. [DOI] [PubMed] [Google Scholar]
  7. Lanham S. M., Lemcke R. M., Scott C. M., Grendon J. M. Isoenzymes in two species of Acholeplasma. J Gen Microbiol. 1980 Mar;117(1):19–31. doi: 10.1099/00221287-117-1-19. [DOI] [PubMed] [Google Scholar]
  8. Liska B., Smith P. F. Requirements of Acholeplasma laidlawii A, strain LA 1, for nucleic acid precursors. Folia Microbiol (Praha) 1974;19(2):107–117. doi: 10.1007/BF02872843. [DOI] [PubMed] [Google Scholar]
  9. McIvor R. S., Kenny G. E. Differences in incorporation of nucleic acid bases and nucleosides by various Mycoplasma and Acholeplasma species. J Bacteriol. 1978 Aug;135(2):483–489. doi: 10.1128/jb.135.2.483-489.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Mitchell A., Finch L. R. Pathways of nucleotide biosynthesis in Mycoplasma mycoides subsp. mycoides. J Bacteriol. 1977 Jun;130(3):1047–1054. doi: 10.1128/jb.130.3.1047-1054.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Mitchell A., Sin I. L., Finch L. R. Enzymes of purine metabolism in Mycoplasma mycoides subsp. mycoides. J Bacteriol. 1978 Jun;134(3):706–712. doi: 10.1128/jb.134.3.706-712.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Muhlrad A., Peleg I., Robertson J. A., Robinson I. M., Kahane I. Acetate kinase activity in mycoplasmas. J Bacteriol. 1981 Jul;147(1):271–273. doi: 10.1128/jb.147.1.271-273.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. O'Brien S. J., Simonson J. M., Grabowski M. W., Barile M. F. Analysis of multiple isoenzyme expression among twenty-two species of Mycoplasma and Acholeplasma. J Bacteriol. 1981 Apr;146(1):222–232. doi: 10.1128/jb.146.1.222-232.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Pollack J. D., Merola A. J., Platz M., Booth R. L., Jr Respiration-associated components of Mollicutes. J Bacteriol. 1981 Jun;146(3):907–913. doi: 10.1128/jb.146.3.907-913.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. RODWELL A. W. Nutrition and metabolism of Mycoplasma mycoides var. mycoides. Ann N Y Acad Sci. 1960 Jan 15;79:499–507. doi: 10.1111/j.1749-6632.1960.tb42716.x. [DOI] [PubMed] [Google Scholar]
  16. Reinards R., Kubicki J., Ohlenbusch H. D. Purification and characterization of NADH oxidase from membranes of Acholeplasma laidlawii, a copper-containing iron-sulfur flavoprotein. Eur J Biochem. 1981 Nov;120(2):329–337. doi: 10.1111/j.1432-1033.1981.tb05708.x. [DOI] [PubMed] [Google Scholar]
  17. SCHIMKE R. T., BARILE M. F. ARGININE METABOLISM IN PLEUROPNEUMONIA-LIKE ORGANISMS ISOLATED FROM MAMMALIAN CELL CULTURE. J Bacteriol. 1963 Aug;86:195–206. doi: 10.1128/jb.86.2.195-206.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. SMITH P. F. Amino acid metabolism by pleuropneumonialike organisms. I. General catabolism. J Bacteriol. 1955 Nov;70(5):552–556. doi: 10.1128/jb.70.5.552-556.1955. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. SMITH S. L., VANDEMARK P. J., FABRICANT J. RESPIRATORY PATHWAYS IN THE MYCOPLASMA. I. LACTATE OXIDATION BY MYCOPLASMA GALLISEPTICUM. J Bacteriol. 1963 Nov;86:893–897. doi: 10.1128/jb.86.5.893-897.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Sin I. L., Finch L. R. Adenine phosphoribosyltransferase in Mycoplasma mycoides and Escherichia coli. J Bacteriol. 1972 Oct;112(1):439–444. doi: 10.1128/jb.112.1.439-444.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Smith D. W., Hanawalt P. C. Macromolecular synthesis and thymineless death in Mycoplasma laidlawii B. J Bacteriol. 1968 Dec;96(6):2066–2076. doi: 10.1128/jb.96.6.2066-2076.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. TOURTELLOTTE M. E., JACOBS R. E. Physiological and serologic comparisons of PPLO from various sources. Ann N Y Acad Sci. 1960 Jan 15;79:521–530. doi: 10.1111/j.1749-6632.1960.tb42718.x. [DOI] [PubMed] [Google Scholar]
  23. TOURTELLOTTE M. E., MOROWITZ H. J., KASIMER P. DEFINED MEDIUM FOR MYCOPLASMA LAIDLAWII. J Bacteriol. 1964 Jul;88:11–15. doi: 10.1128/jb.88.1.11-15.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. VANDEMARK P. J., SMITH P. F. EVIDENCE FOR A TRICARBOXYLIC ACID CYCLE IN MYCOPLASMA HOMINIS. J Bacteriol. 1964 Dec;88:1602–1607. doi: 10.1128/jb.88.6.1602-1607.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. VANDEMARK P. J., SMITH P. F. NATURE OF BUTYRATE OXIDATION BY MYCOPLASMA HOMINIS. J Bacteriol. 1965 Feb;89:373–377. doi: 10.1128/jb.89.2.373-377.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. VANDEMARK P. J., SMITH P. F. RESPIRATORY PATHWAYS IN THE MYCOPLASMA. II. PATHWAY OF ELECTRON TRANSPORT DURING OXIDATION OF REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE BY MYCOPLASMA HOMINIS. J Bacteriol. 1964 Jul;88:122–129. doi: 10.1128/jb.88.1.122-129.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from The Yale Journal of Biology and Medicine are provided here courtesy of Yale Journal of Biology and Medicine

RESOURCES