Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1991 Dec;59(12):4670–4673. doi: 10.1128/iai.59.12.4670-4673.1991

Specific binding of neoglycoproteins to Toxoplasma gondii tachyzoites.

R Robert 1, P L de la Jarrige 1, C Mahaza 1, J Cottin 1, A Marot-Leblond 1, J M Senet 1
PMCID: PMC259094  PMID: 1937826

Abstract

Several studies have shown that protozoa bind to glycoproteins or neoglycoproteins. Here we report that Toxoplasma gondii binds strongly to bovine serum albumin-glucosamide. The binding was rapid, time dependent, partially reversible, saturable, and specific. Scatchard analysis showed about 40,000 molecules of bovine serum albumin-glucosamide per toxoplasma cell. The apparent dissociation constant was found to be 4.46 x 10(-8) M.

Full text

PDF
4672

Images in this article

4671Image
on p.4671

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ardoin P., Couzineau P., Baufine-Ducrocq H. Sur l'utilisation du sarcome T.G. 180 pour l'obtention d'une suspension riche en Toxoplasma gondii extracellulaires. C R Seances Soc Biol Fil. 1967;161(1):117–119. [PubMed] [Google Scholar]
  2. Chadee K., Johnson M. L., Orozco E., Petri W. A., Jr, Ravdin J. I. Binding and internalization of rat colonic mucins by the galactose/N-acetyl-D-galactosamine adherence lectin of Entamoeba histolytica. J Infect Dis. 1988 Aug;158(2):398–406. doi: 10.1093/infdis/158.2.398. [DOI] [PubMed] [Google Scholar]
  3. Cintra W. M., Silva-Filho F. C., De Souza W. The surface charge of Toxoplasma gondii: a cytochemical and electrophoretic study. J Submicrosc Cytol. 1986 Oct;18(4):773–781. [PubMed] [Google Scholar]
  4. Couvreur G., Sadak A., Fortier B., Dubremetz J. F. Surface antigens of Toxoplasma gondii. Parasitology. 1988 Aug;97(Pt 1):1–10. doi: 10.1017/s0031182000066695. [DOI] [PubMed] [Google Scholar]
  5. Crane M. S., Dvorak J. A. Influence of monosaccharides on the infection of vertebrate cells by Trypanosoma cruzi and Toxoplasma gondii. Mol Biochem Parasitol. 1982 May;5(5):333–341. doi: 10.1016/0166-6851(82)90040-8. [DOI] [PubMed] [Google Scholar]
  6. Foussard F., Gallois Y., Tronchin G., Robert R., Mauras G. Isolation of the pellicle of Toxoplasma gondii (Protozoa, Coccidia): characterization by electron microscopy and protein composition. Parasitol Res. 1990;76(7):563–565. doi: 10.1007/BF00932561. [DOI] [PubMed] [Google Scholar]
  7. Fraker P. J., Speck J. C., Jr Protein and cell membrane iodinations with a sparingly soluble chloroamide, 1,3,4,6-tetrachloro-3a,6a-diphrenylglycoluril. Biochem Biophys Res Commun. 1978 Feb 28;80(4):849–857. doi: 10.1016/0006-291x(78)91322-0. [DOI] [PubMed] [Google Scholar]
  8. Handman E., Goding J. W., Remington J. S. Detection and characterization of membrane antigens of Toxoplasma gondii. J Immunol. 1980 Jun;124(6):2578–2583. [PubMed] [Google Scholar]
  9. Hoshino-Shimizu S., Mineo J. R., Camargo M. E. Lectin used in the purification process of Toxoplasma gondii tachyzoites. J Parasitol. 1980 Dec;66(6):989–991. [PubMed] [Google Scholar]
  10. Johnson A. M., McDonald P. J., Neoh S. H. Monoclonal antibodies to Toxoplasma cell membrane surface antigens protect mice from toxoplasmosis. J Protozool. 1983 May;30(2):351–356. doi: 10.1111/j.1550-7408.1983.tb02929.x. [DOI] [PubMed] [Google Scholar]
  11. Johnson A. M. The antigenic structure of Toxoplasma gondii: a review. Pathology. 1985 Jan;17(1):9–19. doi: 10.3109/00313028509063716. [DOI] [PubMed] [Google Scholar]
  12. Jungery M., Boyle D., Patel T., Pasvol G., Weatherall D. J. Lectin-like polypeptides of P. falciparum bind to red cell sialoglycoproteins. Nature. 1983 Feb 24;301(5902):704–705. doi: 10.1038/301704a0. [DOI] [PubMed] [Google Scholar]
  13. Kasper L. H., Crabb J. H., Pfefferkorn E. R. Purification of a major membrane protein of Toxoplasma gondii by immunoabsorption with a monoclonal antibody. J Immunol. 1983 May;130(5):2407–2412. [PubMed] [Google Scholar]
  14. Kobiler D., Mirelman D. Lectin activity in Entamoeba histolytica trophozoites. Infect Immun. 1980 Jul;29(1):221–225. doi: 10.1128/iai.29.1.221-225.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Lev B., Ward H., Keusch G. T., Pereira M. E. Lectin activation in Giardia lamblia by host protease: a novel host-parasite interaction. Science. 1986 Apr 4;232(4746):71–73. doi: 10.1126/science.3513312. [DOI] [PubMed] [Google Scholar]
  16. Mauras G., Dodeur M., Laget P., Senet J. M., Bourrillon R. Partial resolution of the sugar content of Toxoplasma gondii membrane. Biochem Biophys Res Commun. 1980 Dec 16;97(3):906–912. doi: 10.1016/0006-291x(80)91462-x. [DOI] [PubMed] [Google Scholar]
  17. Petri W. A., Jr, Smith R. D., Schlesinger P. H., Murphy C. F., Ravdin J. I. Isolation of the galactose-binding lectin that mediates the in vitro adherence of Entamoeba histolytica. J Clin Invest. 1987 Nov;80(5):1238–1244. doi: 10.1172/JCI113198. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Ravdin J. I., Murphy C. F., Salata R. A., Guerrant R. L., Hewlett E. L. N-Acetyl-D-galactosamine-inhibitable adherence lectin of Entamoeba histolytica. I. Partial purification and relation to amoebic virulence in vitro. J Infect Dis. 1985 May;151(5):804–815. doi: 10.1093/infdis/151.5.804. [DOI] [PubMed] [Google Scholar]
  19. Robert R., Mauras G., Senet J. M., Hocquet P. Purification of Toxoplasma gondii suspensions from mice peritoneal exudate. Biomed Pharmacother. 1982 Mar;36(2):98–100. [PubMed] [Google Scholar]
  20. Rodriguez C., Afchain D., Capron A., Dissous C., Santoro F. Major surface protein of Toxoplasma gondii (p30) contains an immunodominant region with repetitive epitopes. Eur J Immunol. 1985 Jul;15(7):747–749. doi: 10.1002/eji.1830150721. [DOI] [PubMed] [Google Scholar]
  21. Rosales-Encina J. L., Meza I., López-De-León A., Talamás-Rohana P., Rojkind M. Isolation of a 220-kilodalton protein with lectin properties from a virulent strain of Entamoeba histolytica. J Infect Dis. 1987 Nov;156(5):790–797. doi: 10.1093/infdis/156.5.790. [DOI] [PubMed] [Google Scholar]
  22. Santoro F., Charif H., Capron A. The immunodominant epitope of the major membrane tachyzoite protein (P30) of Toxoplasma gondii. Parasite Immunol. 1986 Nov;8(6):631–639. doi: 10.1111/j.1365-3024.1986.tb00876.x. [DOI] [PubMed] [Google Scholar]
  23. Schrevel J., Philippe M., Bernard F., Monsigny M. Surface Plasmodium sugar-binding components evidenced by fluorescent neoglycoproteins. Biol Cell. 1986;56(1):49–55. doi: 10.1111/j.1768-322x.1986.tb00442.x. [DOI] [PubMed] [Google Scholar]
  24. Schulman S., Lee Y. C., Vanderberg J. P. Effects of neoglycoproteins on penetration of Plasmodium falciparum merozoites into erythrocytes in vitro. J Parasitol. 1984 Apr;70(2):213–216. [PubMed] [Google Scholar]
  25. Sethi K. K., Rahman A., Pelster B., Brandis H. Search for the presence of lectin-binding sites on Toxoplasma gondii. J Parasitol. 1977 Dec;63(6):1076–1080. [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES