Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1988 Apr;56(4):972–976. doi: 10.1128/iai.56.4.972-976.1988

Passive protection of chickens against Eimeria tenella infection by monoclonal antibody.

M S Crane 1, P K Murray 1, M J Gnozzio 1, T T MacDonald 1
PMCID: PMC259400  PMID: 3346078

Abstract

Monoclonal antibodies reactive with the surface of Eimeria tenella sporozoites were produced in mice. This paper concerns one of these monoclonal antibodies, designated 1073.10, which agglutinated sporozoites in vitro and lysed the parasite in the presence of complement. This treatment neutralized sporozoite infections when the treated parasites were injected into the ceca of normal chickens. Passive transfer of ammonium sulfate-precipitated 1073.10 ascites fluid into 2- to 3-day-old or 3-week-old chickens conferred protection against challenge infection with E. tenella. These studies show that serum antibody may play a role in immunity to coccidiosis and that the sporozoite surface epitope recognized by 1073.10 is a possible vaccine candidate antigen.

Full text

PDF
975

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Crane M. S., Gnozzio M. J., Murray P. K. Eimeria tenella (Eucoccidiorida): a quantitative assay for sporozoite infectivity in vivo. J Protozool. 1986 Feb;33(1):94–98. doi: 10.1111/j.1550-7408.1986.tb05566.x. [DOI] [PubMed] [Google Scholar]
  2. Crane M. S., Norman D. J., Gnozzio M. J., Tate A. C., Gammon M., Murray P. K. Eimeria tenella: quantitative in vitro and in vivo studies on the effects of mouse polyclonal and monoclonal antibodies on sporozoites. Parasite Immunol. 1986 Sep;8(5):467–480. doi: 10.1111/j.1365-3024.1986.tb00862.x. [DOI] [PubMed] [Google Scholar]
  3. Danforth H. D. Development of hybridoma-produced antibodies directed against Eimeria tenella and E. mitis. J Parasitol. 1982 Jun;68(3):392–397. [PubMed] [Google Scholar]
  4. Danforth H. D. Use of monoclonal antibodies directed against Eimeria tenella sporozoites to determine stage specificity and in vitro effect on parasite penetration and development. Am J Vet Res. 1983 Sep;44(9):1722–1727. [PubMed] [Google Scholar]
  5. Davis P. J., Parry S. H., Porter P. The role of secretory IgA in anti-coccidial immunity in the chicken. Immunology. 1978 May;34(5):879–888. [PMC free article] [PubMed] [Google Scholar]
  6. Davis P. J., Porter P. A mechanism for secretory IgA-mediated inhibition of the cell penetration and intracellular development of Eimeria tenella. Immunology. 1979 Mar;36(3):471–477. [PMC free article] [PubMed] [Google Scholar]
  7. Ey P. L., Prowse S. J., Jenkin C. R. Isolation of pure IgG1, IgG2a and IgG2b immunoglobulins from mouse serum using protein A-sepharose. Immunochemistry. 1978 Jul;15(7):429–436. doi: 10.1016/0161-5890(78)90070-6. [DOI] [PubMed] [Google Scholar]
  8. Grzych J. M., Capron M., Lambert P. H., Dissous C., Torres S., Capron A. An anti-idiotype vaccine against experimental schistosomiasis. Nature. 1985 Jul 4;316(6023):74–76. doi: 10.1038/316074a0. [DOI] [PubMed] [Google Scholar]
  9. Herlich H. Effect of chicken antiserum and tissue extracts on the oocysts, sporozoites, and merozoites of Eimeria tenella and E. acervulina. J Parasitol. 1965 Oct;51(5):847–851. [PubMed] [Google Scholar]
  10. Johnson J., Reid W. M. Anticoccidial drugs: lesion scoring techniques in battery and floor-pen experiments with chickens. Exp Parasitol. 1970 Aug;28(1):30–36. doi: 10.1016/0014-4894(70)90063-9. [DOI] [PubMed] [Google Scholar]
  11. Joyner L. P., Norton C. C. The effect of early cyclophosphamide treatment on the subsequent immunity of chickens to coccidial infections. Parasitology. 1974 Jun;68(3):323–330. doi: 10.1017/s0031182000045856. [DOI] [PubMed] [Google Scholar]
  12. LONG P. L., ROSE M. E., PIERCE A. E. EFFECTS OF FOWL SERA ON SOME STAGES IN THE LIFE CYCLE OF EIMERIA TENELLA. Exp Parasitol. 1963 Oct;14:210–217. doi: 10.1016/0014-4894(63)90025-0. [DOI] [PubMed] [Google Scholar]
  13. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  14. Lawn A. M., Rose M. E. Mucosal transport of Eimeria tenella in the cecum of the chicken. J Parasitol. 1982 Dec;68(6):1117–1123. [PubMed] [Google Scholar]
  15. Long P. L., Rose M. E. Immunity to coccidiosis: effect of serum antibodies on cell invasion by sporozoites of Eimeria in vitro. Parasitology. 1972 Dec;65(3):437–445. doi: 10.1017/s0031182000044061. [DOI] [PubMed] [Google Scholar]
  16. PIERCE A. E., LONG L. P., HORTON-SMITH C. Attempts to induce a passive immunity to Eimeria tenella in young fowls (Gallus domesticus). Immunology. 1963 Jan;6:37–47. [PMC free article] [PubMed] [Google Scholar]
  17. ROSE M. E., LONG P. L. Immunity to four species of Eimeria in fowls. Immunology. 1962 Jan;5:79–92. [PMC free article] [PubMed] [Google Scholar]
  18. Reagan K. J., Wunner W. H., Wiktor T. J., Koprowski H. Anti-idiotypic antibodies induce neutralizing antibodies to rabies virus glycoprotein. J Virol. 1983 Dec;48(3):660–666. doi: 10.1128/jvi.48.3.660-666.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Rose M. E., Hesketh P. Immunity to coccidia in chickens: adoptive transfer with peripheral blood lymphocytes and spleen cells. Parasite Immunol. 1982 May;4(3):171–185. doi: 10.1111/j.1365-3024.1982.tb00429.x. [DOI] [PubMed] [Google Scholar]
  20. Rose M. E., Hesketh P. Immunity to coccidiosis: T-lymphocyte- or B-lymphocyte-deficient animals. Infect Immun. 1979 Nov;26(2):630–637. doi: 10.1128/iai.26.2.630-637.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Rose M. E. Immunity to Eimeria maxima: reactions of antisera in vitro and protection in vivo. J Parasitol. 1974 Jun;60(3):528–530. [PubMed] [Google Scholar]
  22. Rose M. E. Immunity to coccidiosis: protective effect of transferred serum in Eimeria maxima infections. Parasitology. 1971 Feb;62(1):11–25. doi: 10.1017/s0031182000071249. [DOI] [PubMed] [Google Scholar]
  23. Rose M. E., Long P. L. Immunity to coccidiosis: gut permeability changes in response to sporozoite invasion. Experientia. 1969 Feb 15;25(2):183–184. doi: 10.1007/BF01899112. [DOI] [PubMed] [Google Scholar]
  24. Rose M. E., Long P. L. Immunity to coccidiosis: protective effects of transferred serum and cells investigated in chick embryos infected with Eimeria tenella. Parasitology. 1971 Oct;63(2):299–313. doi: 10.1017/s0031182000079610. [DOI] [PubMed] [Google Scholar]
  25. Sacks D. L., Esser K. M., Sher A. Immunization of mice against African trypanosomiasis using anti-idiotypic antibodies. J Exp Med. 1982 Apr 1;155(4):1108–1119. doi: 10.1084/jem.155.4.1108. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Schmatz D. M., Crane M. S., Murray P. K. Purification of Eimeria sporozoites by DE-52 anion exchange chromatography. J Protozool. 1984 Feb;31(1):181–183. doi: 10.1111/j.1550-7408.1984.tb04314.x. [DOI] [PubMed] [Google Scholar]
  27. Witlock D. R., Danforth H. D. Surface changes induced by immune serum on Eimeria tenella sporozoites and merozoites. J Protozool. 1982 Aug;29(3):441–445. doi: 10.1111/j.1550-7408.1982.tb05429.x. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES