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. 1988 Aug;56(8):2180–2188. doi: 10.1128/iai.56.8.2180-2188.1988

CS31A, a new K88-related fimbrial antigen on bovine enterotoxigenic and septicemic Escherichia coli strains.

J P Girardeau 1, M Der Vartanian 1, J L Ollier 1, M Contrepois 1
PMCID: PMC259542  PMID: 2899553

Abstract

The nature of the common surface antigen of six hemagglutinating and adhesive piliated Escherichia coli strains isolated from diarrheic or septicemic calves was studied. By electron microscopy studies, the E. coli surface antigen designated CS31A was found on bacterial cells and in purified form to consist of thin (2-nm) "fibrillar" fimbriae. E. coli 31A, which was cured of a 105-megadalton plasmid, failed to express CS31A fimbriae, but retained the ability to hemagglutinate and to adhere in vitro on intestinal cells. Conversely, E. coli K-12, harboring the 105-megadalton plasmid originating from strain 31A, produced CS31A fimbriae but was not able to hemagglutinate or adhere on intestinal cells. A single fimbrial subunit of 29 kilodaltons was observed when purified fimbriae from the 105-megadalton plasmid-containing E. coli K-12 strain was subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis or eluted by gel filtration after dissociation by 8.5 M guanidium hydrochloride from an S300 Sephacryl column. Western immunoblot analysis and the N-terminal sequence and amino acid composition of CS31A indicate structural and immunological relatedness between CS31A and K88 protein subunits.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Contrepois M. G., Girardeau J. P. Additive protective effects of colostral antipili antibodies in calves experimentally infected with enterotoxigenic Escherichia coli. Infect Immun. 1985 Dec;50(3):947–949. doi: 10.1128/iai.50.3.947-949.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Contrepois M., Dubourguier H. C., Parodi A. L., Girardeau J. P., Ollier J. L. Septicaemic Escherichia coli and experimental infection of calves. Vet Microbiol. 1986 Jul;12(2):109–118. doi: 10.1016/0378-1135(86)90073-8. [DOI] [PubMed] [Google Scholar]
  3. Contrepois M., Martel J. L., Bordas C., Hayers F., Millet A., Ramisse J., Sendral R. Fréquence des pili FY et K99 parmi des souches de Escherichia coli isolées de veaux diarrhéiques en France. Ann Rech Vet. 1985;16(1):25–28. [PubMed] [Google Scholar]
  4. Darfeuille-Michaud A., Forestier C., Joly B., Cluzel R. Identification of a nonfimbrial adhesive factor of an enterotoxigenic Escherichia coli strain. Infect Immun. 1986 May;52(2):468–475. doi: 10.1128/iai.52.2.468-475.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. De Rycke J. Röle des souches d'Escherichia coli non enterotoxinogènes (K99-, ST-) dans la pathologie-neonatale du veau. Ann Rech Vet. 1984;15(1):75–95. [PubMed] [Google Scholar]
  6. Dean A. G., Ching Y. C., Williams R. G., Harden L. B. Test for Escherichia coli enterotoxin using infant mice: application in a study of diarrhea in children in Honolulu. J Infect Dis. 1972 Apr;125(4):407–411. doi: 10.1093/infdis/125.4.407. [DOI] [PubMed] [Google Scholar]
  7. Eshdat Y., Silverblatt F. J., Sharon N. Dissociation and reassembly of Escherichia coli type 1 pili. J Bacteriol. 1981 Oct;148(1):308–314. doi: 10.1128/jb.148.1.308-314.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Evans D. G., Silver R. P., Evans D. J., Jr, Chase D. G., Gorbach S. L. Plasmid-controlled colonization factor associated with virulence in Esherichia coli enterotoxigenic for humans. Infect Immun. 1975 Sep;12(3):656–667. doi: 10.1128/iai.12.3.656-667.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Evans D. J., Jr, Evans D. G., DuPont H. L. Hemagglutination patterns of enterotoxigenic and enteropathogenic Escherichia coli determined with human, bovine, chicken, and guinea pig erythrocytes in the presence and absence of mannose. Infect Immun. 1979 Feb;23(2):336–346. doi: 10.1128/iai.23.2.336-346.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Gaastra W., de Graaf F. K. Host-specific fimbrial adhesins of noninvasive enterotoxigenic Escherichia coli strains. Microbiol Rev. 1982 Jun;46(2):129–161. doi: 10.1128/mr.46.2.129-161.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Gay C. C., McGuire T. C., Parish S. M. Seasonal variation in passive transfer of immunoglobulin G1 to newborn calves. J Am Vet Med Assoc. 1983 Sep 1;183(5):566–568. [PubMed] [Google Scholar]
  12. Girardeau J. P. A new in vitro technique for attachment to intestinal villi using enteropathogenic Escherichia coli. Ann Microbiol (Paris) 1980 Jul-Aug;131B(1):31–37. [PubMed] [Google Scholar]
  13. Girardeau J. P., Dubourguier H. C., Gouet P. Effect of glucose and amino acids on expression of K99 antigen in Escherichia coli. J Gen Microbiol. 1982 Oct;128(10):2243–2249. doi: 10.1099/00221287-128-10-2243. [DOI] [PubMed] [Google Scholar]
  14. Guinée P. A., Jansen W. H., Agterberg C. M. Detection of the K99 antigen by means of agglutination and immunoelectrophoresis in Escherichia coli isolates from calves and its correlation with entertoxigenicity. Infect Immun. 1976 May;13(5):1369–1377. doi: 10.1128/iai.13.5.1369-1377.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Guinée P. A., Mooi F. R., Jansen W. H. Preparation of specific Escherichia coli K88 antisera by means of purified K88ab and K88ad antigens. Zentralbl Bakteriol A. 1980 Nov;248(2):182–189. [PubMed] [Google Scholar]
  16. Hewick R. M., Hunkapiller M. W., Hood L. E., Dreyer W. J. A gas-liquid solid phase peptide and protein sequenator. J Biol Chem. 1981 Aug 10;256(15):7990–7997. [PubMed] [Google Scholar]
  17. Hill A. W. Factors influencing the outcome of Escherichia coli mastitis in the dairy cow. Res Vet Sci. 1981 Jul;31(1):107–112. [PubMed] [Google Scholar]
  18. Kado C. I., Liu S. T. Rapid procedure for detection and isolation of large and small plasmids. J Bacteriol. 1981 Mar;145(3):1365–1373. doi: 10.1128/jb.145.3.1365-1373.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Klemm P. Fimbrial adhesions of Escherichia coli. Rev Infect Dis. 1985 May-Jun;7(3):321–340. doi: 10.1093/clinids/7.3.321. [DOI] [PubMed] [Google Scholar]
  20. Klemm P., Mikkelsen L. Prediction of antigenic determinants and secondary structures of the K88 and CFA1 fimbrial proteins from enteropathogenic Escherichia coli. Infect Immun. 1982 Oct;38(1):41–45. doi: 10.1128/iai.38.1.41-45.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Levine M. M. Escherichia coli that cause diarrhea: enterotoxigenic, enteropathogenic, enteroinvasive, enterohemorrhagic, and enteroadherent. J Infect Dis. 1987 Mar;155(3):377–389. doi: 10.1093/infdis/155.3.377. [DOI] [PubMed] [Google Scholar]
  22. Levine M. M., Kaper J. B., Black R. E., Clements M. L. New knowledge on pathogenesis of bacterial enteric infections as applied to vaccine development. Microbiol Rev. 1983 Dec;47(4):510–550. doi: 10.1128/mr.47.4.510-550.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Levine M. M., Ristaino P., Marley G., Smyth C., Knutton S., Boedeker E., Black R., Young C., Clements M. L., Cheney C. Coli surface antigens 1 and 3 of colonization factor antigen II-positive enterotoxigenic Escherichia coli: morphology, purification, and immune responses in humans. Infect Immun. 1984 May;44(2):409–420. doi: 10.1128/iai.44.2.409-420.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Markwell M. A., Haas S. M., Bieber L. L., Tolbert N. E. A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples. Anal Biochem. 1978 Jun 15;87(1):206–210. doi: 10.1016/0003-2697(78)90586-9. [DOI] [PubMed] [Google Scholar]
  25. McGuire T. C., Pfeiffer N. E., Weikel J. M., Bartsch R. C. Failure of colostral immunoglobulin transfer in calves dying from infectious disease. J Am Vet Med Assoc. 1976 Oct 1;169(7):713–718. [PubMed] [Google Scholar]
  26. Mooi F. R., de Graaf F. K. Molecular biology of fimbriae of enterotoxigenic Escherichia coli. Curr Top Microbiol Immunol. 1985;118:119–138. doi: 10.1007/978-3-642-70586-1_7. [DOI] [PubMed] [Google Scholar]
  27. Morris J. A., Thorns C. J., Sojka W. J. Evidence for two adhesive antigens on the K99 reference strain Escherichia coli B41. J Gen Microbiol. 1980 May;118(1):107–113. doi: 10.1099/00221287-118-1-107. [DOI] [PubMed] [Google Scholar]
  28. Morrissey J. H. Silver stain for proteins in polyacrylamide gels: a modified procedure with enhanced uniform sensitivity. Anal Biochem. 1981 Nov 1;117(2):307–310. doi: 10.1016/0003-2697(81)90783-1. [DOI] [PubMed] [Google Scholar]
  29. Oakley B. R., Kirsch D. R., Morris N. R. A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels. Anal Biochem. 1980 Jul 1;105(2):361–363. doi: 10.1016/0003-2697(80)90470-4. [DOI] [PubMed] [Google Scholar]
  30. Ollier J. L., Girardeau J. P. Structures et fonctions hémagglutinantes des pili K99 dont la biosynthèse est glucose-dépendante ou constitutive et des pili F41. Ann Microbiol (Paris) 1983 May-Jun;134A(3):247–254. [PubMed] [Google Scholar]
  31. Orskov I., Birch-Andersen A., Duguid J. P., Stenderup J., Orskov F. An adhesive protein capsule of Escherichia coli. Infect Immun. 1985 Jan;47(1):191–200. doi: 10.1128/iai.47.1.191-200.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Smyth C. J. Two mannose-resistant haemagglutinins on enterotoxigenic Escherichia coli of serotype O6:K15:H16 or H-isolated from travellers' and infantile diarrhoea. J Gen Microbiol. 1982 Sep;128(9):2081–2096. doi: 10.1099/00221287-128-9-2081. [DOI] [PubMed] [Google Scholar]
  33. Stirm S., Orskov F., Orskov I., Birch-Andersen A. Episome-carried surface antigen K88 of Escherichia coli. 3. Morphology. J Bacteriol. 1967 Feb;93(2):740–748. doi: 10.1128/jb.93.2.740-748.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Wickstrom M. L., Gay C. C., Hodgson J. L., Widders P. R., Schaeffer D., Lee R., Corbeil L. B. Cross-reactive antibody in immunity to colisepticemia in calves. Vet Microbiol. 1987 Mar;13(3):259–271. doi: 10.1016/0378-1135(87)90088-5. [DOI] [PubMed] [Google Scholar]
  36. Ziegler E. J., McCutchan J. A., Braude A. I. Treatment of gram-negative bacteremia with antiserum to core glycolipid. I. The experimental basis of immunity to endotoxin. Eur J Cancer. 1979;15 (Suppl):71–76. [PubMed] [Google Scholar]
  37. Zimmerman C. L., Appella E., Pisano J. J. Rapid analysis of amino acid phenylthiohydantoins by high-performance liquid chromatography. Anal Biochem. 1977 Feb;77(2):569–573. doi: 10.1016/0003-2697(77)90276-7. [DOI] [PubMed] [Google Scholar]
  38. de Graaf F. K., Klaasen-Boor P., van Hees J. E. Biosynthesis of the K99 surface antigen is repressed by alanine. Infect Immun. 1980 Oct;30(1):125–128. doi: 10.1128/iai.30.1.125-128.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  39. de Graaf F. K., Roorda I. Production, purification, and characterization of the fimbrial adhesive antigen F41 isolated from calf enteropathogenic Escherichia coli strain B41M. Infect Immun. 1982 May;36(2):751–758. doi: 10.1128/iai.36.2.751-758.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]

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