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. 1988 Nov;56(11):2866–2870. doi: 10.1128/iai.56.11.2866-2870.1988

Tn916 insertional inactivation of multiple genes on the chromosome of Streptococcus mutans GS-5.

J K Procino 1, L Marri 1, G D Shockman 1, L Daneo-Moore 1
PMCID: PMC259663  PMID: 2844674

Abstract

Streptococcus mutans GS-5 was transformed with the Escherichia coli plasmid pAM150 containing the cloned streptococcal transposon Tn916. Southern blot analyses with the tetracycline-resistant determinant of Tn916 showed that Tn916 was inserted into the chromosome of S. mutans at a variety of different sites. Tn916 insertions resulted in the inactivation of genes that code for various steps in the biosynthesis of several different amino acids. Two auxotrophs which contained a single copy of Tn916 were shown to revert to prototrophy at frequencies of about 10(-8). All of the revertant prototrophs were susceptible to tetracycline, indicating regeneration of the functional gene by excision of Tn916.

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These references are in PubMed. This may not be the complete list of references from this article.

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