Skip to main content
Infection and Immunity logoLink to Infection and Immunity
. 1989 Nov;57(11):3265–3269. doi: 10.1128/iai.57.11.3265-3269.1989

Surface location of a Bacteroides gingivalis glycylprolyl protease.

D Grenier 1, B C McBride 1
PMCID: PMC259793  PMID: 2807524

Abstract

Various immunological methods were used to localize a glycylprolyl protease previously isolated from Bacteroides gingivalis ATCC 33277. The results obtained by enzyme-linked immunosorbent assay, indirect immunofluorescence staining, and indirect immunogold labeling suggest that the glycylprolyl protease is present on the surface of the cell outer membrane and is specific to B. gingivalis strains. The enzyme was removed from the cell envelope by treatment of the whole cells with sodium dodecyl sulfate, Triton X-100, sodium deoxycholate, and proteinase K.

Full text

PDF
3267

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abiko Y., Hayakawa M., Murai S., Takiguchi H. Glycylprolyl dipeptidylaminopeptidase from Bacteroides gingivalis. J Dent Res. 1985 Feb;64(2):106–111. doi: 10.1177/00220345850640020201. [DOI] [PubMed] [Google Scholar]
  2. Burnette W. N. "Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate--polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Anal Biochem. 1981 Apr;112(2):195–203. doi: 10.1016/0003-2697(81)90281-5. [DOI] [PubMed] [Google Scholar]
  3. Carlsson J., Herrmann B. F., Höfling J. F., Sundqvist G. K. Degradation of the human proteinase inhibitors alpha-1-antitrypsin and alpha-2-macroglobulin by Bacteroides gingivalis. Infect Immun. 1984 Feb;43(2):644–648. doi: 10.1128/iai.43.2.644-648.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Carlsson J., Höfling J. F., Sundqvist G. K. Degradation of albumin, haemopexin, haptoglobin and transferrin, by black-pigmented Bacteroides species. J Med Microbiol. 1984 Aug;18(1):39–46. doi: 10.1099/00222615-18-1-39. [DOI] [PubMed] [Google Scholar]
  5. Darveau R. P., Hancock R. E. Procedure for isolation of bacterial lipopolysaccharides from both smooth and rough Pseudomonas aeruginosa and Salmonella typhimurium strains. J Bacteriol. 1983 Aug;155(2):831–838. doi: 10.1128/jb.155.2.831-838.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Foltmann B., Szecsi P. B., Tarasova N. I. Detection of proteases by clotting of casein after gel electrophoresis. Anal Biochem. 1985 May 1;146(2):353–360. doi: 10.1016/0003-2697(85)90551-2. [DOI] [PubMed] [Google Scholar]
  7. Fujimura S., Nakamura T. Isolation and characterization of a protease from Bacteroides gingivalis. Infect Immun. 1987 Mar;55(3):716–720. doi: 10.1128/iai.55.3.716-720.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. GIBBONS R. J., MACDONALD J. B. Degradation of collagenous substrates by Bacteroides melaninogenicus. J Bacteriol. 1961 Apr;81:614–621. doi: 10.1128/jb.81.4.614-621.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Grenier D., Chao G., McBride B. C. Characterization of sodium dodecyl sulfate-stable Bacteroides gingivalis proteases by polyacrylamide gel electrophoresis. Infect Immun. 1989 Jan;57(1):95–99. doi: 10.1128/iai.57.1.95-99.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Grenier D., Mayrand D. Functional characterization of extracellular vesicles produced by Bacteroides gingivalis. Infect Immun. 1987 Jan;55(1):111–117. doi: 10.1128/iai.55.1.111-117.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Grenier D., McBride B. C. Isolation of a membrane-associated Bacteroides gingivalis glycylprolyl protease. Infect Immun. 1987 Dec;55(12):3131–3136. doi: 10.1128/iai.55.12.3131-3136.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kilian M. Degradation of immunoglobulins A2, A2, and G by suspected principal periodontal pathogens. Infect Immun. 1981 Dec;34(3):757–765. doi: 10.1128/iai.34.3.757-765.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  14. Slots J., Genco R. J. Black-pigmented Bacteroides species, Capnocytophaga species, and Actinobacillus actinomycetemcomitans in human periodontal disease: virulence factors in colonization, survival, and tissue destruction. J Dent Res. 1984 Mar;63(3):412–421. doi: 10.1177/00220345840630031101. [DOI] [PubMed] [Google Scholar]
  15. Suido H., Neiders M. E., Barua P. K., Nakamura M., Mashimo P. A., Genco R. J. Characterization of N-CBz-glycyl-glycyl-arginyl peptidase and glycyl-prolyl peptidase of Bacteroides gingivalis. J Periodontal Res. 1987 Sep;22(5):412–418. doi: 10.1111/j.1600-0765.1987.tb01608.x. [DOI] [PubMed] [Google Scholar]
  16. Sundqvist G., Carlsson J., Herrmann B., Tärnvik A. Degradation of human immunoglobulins G and M and complement factors C3 and C5 by black-pigmented Bacteroides. J Med Microbiol. 1985 Feb;19(1):85–94. doi: 10.1099/00222615-19-1-85. [DOI] [PubMed] [Google Scholar]
  17. Tsutsui H., Kinouchi T., Wakano Y., Ohnishi Y. Purification and characterization of a protease from Bacteroides gingivalis 381. Infect Immun. 1987 Feb;55(2):420–427. doi: 10.1128/iai.55.2.420-427.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. White D., Mayrand D. Association of oral Bacteroides with gingivitis and adult periodontitis. J Periodontal Res. 1981 May;16(3):259–265. doi: 10.1111/j.1600-0765.1981.tb00974.x. [DOI] [PubMed] [Google Scholar]
  19. Yoshimura F., Nishikata M., Suzuki T., Hoover C. I., Newbrun E. Characterization of a trypsin-like protease from the bacterium Bacteroides gingivalis isolated from human dental plaque. Arch Oral Biol. 1984;29(7):559–564. doi: 10.1016/0003-9969(84)90078-5. [DOI] [PubMed] [Google Scholar]
  20. Zambon J. J., Reynolds H. S., Slots J. Black-pigmented Bacteroides spp. in the human oral cavity. Infect Immun. 1981 Apr;32(1):198–203. doi: 10.1128/iai.32.1.198-203.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES