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. 1989 Dec;57(12):3945–3948. doi: 10.1128/iai.57.12.3945-3948.1989

Spontaneous switching of the sucrose-promoted colony phenotype in Streptococcus sanguis.

G Tardif 1, M C Sulavik 1, G W Jones 1, D B Clewell 1
PMCID: PMC259931  PMID: 2530177

Abstract

Streptococcus sanguis on media containing 3% sucrose gives rise to characteristic hard cohesive colonies (designated Spp+). Populations of Spp+ bacteria (strain Challis) on sucrose media switch to a soft noncohesive phenotype (designated Spp-) at a frequency of 10(-4) to 10(-3). Spp- bacteria switch back to Spp+ bacteria at a similar frequency. Successive rounds of Spp variation were observed. The Spp phenotypic switch was associated with changes in extracellular glucosyltransferase activity.

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Selected References

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  1. Bayliss R., Clarke C., Oakley C. M., Somerville W., Whitfield A. G., Young S. E. The microbiology and pathogenesis of infective endocarditis. Br Heart J. 1983 Dec;50(6):513–519. doi: 10.1136/hrt.50.6.513. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Carlsson J., Grahnén H., Jonsson G., Wikner S. Establishment of Streptococcus sanguis in the mouths of infants. Arch Oral Biol. 1970 Dec;15(12):1143–1148. doi: 10.1016/0003-9969(70)90005-1. [DOI] [PubMed] [Google Scholar]
  3. Carlsson J., Newbrun E., Krasse B. Purification and properties of dextransucrase from Streptococcus sanguis. Arch Oral Biol. 1969 May;14(5):469–478. doi: 10.1016/0003-9969(69)90140-x. [DOI] [PubMed] [Google Scholar]
  4. Carlsson J. Zooglea-forming streptococci, resembling Streptococcus sanguis, isolated from dental plaque in man. Odontol Revy. 1965;16(4):348–358. [PubMed] [Google Scholar]
  5. Drucker D. B., Shakespeare A. P., Green R. M. In-vivo dental plaque-forming ability and relative cariogenicity of the bacteria Streptococcus mitis and Streptococcus sanguis I and II in mono-infected gnotobiotic rats. Arch Oral Biol. 1984;29(12):1023–1031. doi: 10.1016/0003-9969(84)90150-x. [DOI] [PubMed] [Google Scholar]
  6. Dummer P. M., Green R. M. A comparison of the ability of strains of streptococci to form dental plaque-like deposits in vitro with their cariogenicity in gnotobiotic rats. Arch Oral Biol. 1980;25(4):245–249. doi: 10.1016/0003-9969(80)90029-1. [DOI] [PubMed] [Google Scholar]
  7. Furuta T., Koga T., Nisizawa T., Okahashi N., Hamada S. Purification and characterization of glucosyltransferases from Streptococcus mutans 6715. J Gen Microbiol. 1985 Feb;131(2):285–293. doi: 10.1099/00221287-131-2-285. [DOI] [PubMed] [Google Scholar]
  8. Gibbons R. J., Nygaard M. Synthesis of insoluble dextran and its significance in the formation of gelatinous deposits by plaque-forming streptococci. Arch Oral Biol. 1968 Oct;13(10):1249–1262. doi: 10.1016/0003-9969(68)90081-2. [DOI] [PubMed] [Google Scholar]
  9. Grahame D. A., Mayer R. M. Purification, and comparison, of two forms of dextransucrase from Streptococcus sanguis. Carbohydr Res. 1985 Oct 15;142(2):285–298. doi: 10.1016/0008-6215(85)85030-8. [DOI] [PubMed] [Google Scholar]
  10. Hamada S., Slade H. D. Biology, immunology, and cariogenicity of Streptococcus mutans. Microbiol Rev. 1980 Jun;44(2):331–384. doi: 10.1128/mr.44.2.331-384.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hamada S., Torii M., Kotani S., Tsuchitani Y. Adherence of Streptococcus sanguis clinical isolates to smooth surfaces and interactions of the isolates with Streptococcus mutans glucosyltransferase. Infect Immun. 1981 Apr;32(1):364–372. doi: 10.1128/iai.32.1.364-372.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Keevil C. W., West A. A., Bourne N., Marsh P. D. Inhibition of the synthesis and secretion of extracellular glucosyl- and fructosyltransferase in Streptococcus sanguis by sodium ions. J Gen Microbiol. 1984 Jan;130(1):77–82. doi: 10.1099/00221287-130-1-77. [DOI] [PubMed] [Google Scholar]
  13. Kuramitsu H. K. Characterization of extracellular glucosyltransferase activity of Steptococcus mutans. Infect Immun. 1975 Oct;12(4):738–749. doi: 10.1128/iai.12.4.738-749.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  15. Loesche W. J. Role of Streptococcus mutans in human dental decay. Microbiol Rev. 1986 Dec;50(4):353–380. doi: 10.1128/mr.50.4.353-380.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Niven C. F., Kiziuta Z., White J. C. Synthesis of a Polysaccharide from Sucrose by Streptococcus S.B.E. J Bacteriol. 1946 Jun;51(6):711–716. doi: 10.1128/jb.51.6.711-716.1946. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Plasterk R. H., Van de Putte P. Genetic switches by DNA inversions in prokaryotes. Biochim Biophys Acta. 1984 Jun 16;782(2):111–119. doi: 10.1016/0167-4781(84)90013-7. [DOI] [PubMed] [Google Scholar]
  18. Russell R. R. Use of triton X-100 to overcome the inhibition of fructosyltransferase by SDS. Anal Biochem. 1979 Aug;97(1):173–175. doi: 10.1016/0003-2697(79)90342-7. [DOI] [PubMed] [Google Scholar]
  19. Stibitz S., Aaronson W., Monack D., Falkow S. Phase variation in Bordetella pertussis by frameshift mutation in a gene for a novel two-component system. Nature. 1989 Mar 16;338(6212):266–269. doi: 10.1038/338266a0. [DOI] [PubMed] [Google Scholar]
  20. Tsumori H., Shimamura A., Mukasa H. Purification and properties of extracellular glucosyltransferase synthesizing 1,3-alpha-D-glucan from Streptococcus mutans serotype a. J Gen Microbiol. 1985 Mar;131(3):553–559. doi: 10.1099/00221287-131-3-553. [DOI] [PubMed] [Google Scholar]
  21. Ueda S., Kuramitsu H. K. Molecular basis for the spontaneous generation of colonization-defective mutants of Streptococcus mutans. Mol Microbiol. 1988 Jan;2(1):135–140. doi: 10.1111/j.1365-2958.1988.tb00014.x. [DOI] [PubMed] [Google Scholar]
  22. Van Houte J., Gibbons R. J., Pulkkinen A. J. Adherence as an ecological determinant for streptococci in the human mouth. Arch Oral Biol. 1971 Oct;16(10):1131–1141. doi: 10.1016/0003-9969(71)90042-2. [DOI] [PubMed] [Google Scholar]
  23. Zacharius R. M., Zell T. E., Morrison J. H., Woodlock J. J. Glycoprotein staining following electrophoresis on acrylamide gels. Anal Biochem. 1969 Jul;30(1):148–152. doi: 10.1016/0003-2697(69)90383-2. [DOI] [PubMed] [Google Scholar]
  24. de Stoppelaar J. D., van Houte J., de Moor C. E. The presence of dextran-forming bacteria, resembling Streptococcus bovis and Streptococcus anguis, in human dental plaque. Arch Oral Biol. 1967 Oct;12(10):1199–1202. doi: 10.1016/0003-9969(67)90069-6. [DOI] [PubMed] [Google Scholar]

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