Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1987 Dec;55(12):3006–3010. doi: 10.1128/iai.55.12.3006-3010.1987

Structural studies of the serotype-f polysaccharide antigen from Streptococcus mutans OMZ175.

R Linzer 1, M S Reddy 1, M J Levine 1
PMCID: PMC260020  PMID: 2824381

Abstract

The serotype f antigen of Streptococcus mutans has been described as a rhamnose-glucose polysaccharide associated with the bacterial cell wall. In this study, the structure of serotype f polysaccharide was examined by analyses of the methylated derivatives of the antigen and the periodate-oxidized antigen. Methylated derivatives were characterized with a gas chromatograph-mass spectrometer. The polysaccharide appeared to have a backbone of alternating 1,3- and 1,2,3-linked rhamnose units. Branching occurred at the 3-position of the 1,2,3-linked rhamnose. Side chains were composed of terminal alpha-linked glucose units. A small proportion of longer side chains containing 1,2- and 1,6-linked glucose units were noted in some preparations; however, these determinants were not reactive with serotype f antisera.

Full text

PDF
3010

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Coligan J. E., Schnute W. C., Jr, Kindt T. J. Immunochemical and chemical studies on streptococcal group-specific carbohydrates. J Immunol. 1975 Jun;114(6):1654–1658. [PubMed] [Google Scholar]
  2. HAKOMORI S. A RAPID PERMETHYLATION OF GLYCOLIPID, AND POLYSACCHARIDE CATALYZED BY METHYLSULFINYL CARBANION IN DIMETHYL SULFOXIDE. J Biochem. 1964 Feb;55:205–208. [PubMed] [Google Scholar]
  3. Hamada S., Gill K., Slade H. D. Chemical and immunological properties of the type f polysaccharide antigen of Streptococcus mutans. Infect Immun. 1976 Jul;14(1):203–211. doi: 10.1128/iai.14.1.203-211.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hamada S., Slade H. D. Purification and immunochemical characterization of type e polysaccharide antigen of Streptococcus mutans. Infect Immun. 1976 Jul;14(1):68–76. doi: 10.1128/iai.14.1.68-76.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Knox K. W., Campbell L. K., Bratthall D. Detection of antigens in enzymic lysates of cell wall from Streptococcus mutans strains. J Dent Res. 1983 Oct;62(10):1033–1037. doi: 10.1177/00220345830620100401. [DOI] [PubMed] [Google Scholar]
  6. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  7. Linzer R., Campbell L. K., Knox K. W. Characterization of polysaccharide antigens of Streptococcus mutans B13 grown under various conditions. Infect Immun. 1984 Apr;44(1):76–81. doi: 10.1128/iai.44.1.76-81.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Linzer R., Reddy M. S., Levine M. J. Structural studies of the rhamnose-glucose polysaccharide antigen from Streptococcus sobrinus B13 and 6715-T2. Infect Immun. 1985 Nov;50(2):583–585. doi: 10.1128/iai.50.2.583-585.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Stellner K., Saito H., Hakomori S. I. Determination of aminosugar linkages in glycolipids by methylation. Aminosugar linkages of ceramide pentasaccharides of rabbit erythrocytes and of Forssman antigen. Arch Biochem Biophys. 1973 Apr;155(2):464–472. doi: 10.1016/0003-9861(73)90138-0. [DOI] [PubMed] [Google Scholar]
  10. Tabak L. A., Reddy M. S., Levine M. J. Characterization of a pentasaccharide in salivary mucin from the stumptail monkey, Macaca arctoides. Arch Oral Biol. 1982;27(4):297–303. doi: 10.1016/0003-9969(82)90158-3. [DOI] [PubMed] [Google Scholar]
  11. Wachsmann D., Klein J. P., Scholler M., Ogier J., Ackermans F., Frank R. M. Serum and salivary antibody responses in rats orally immunized with Streptococcus mutans carbohydrate protein conjugate associated with liposomes. Infect Immun. 1986 May;52(2):408–413. doi: 10.1128/iai.52.2.408-413.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Wetherell J. F., Jr, Bleiweis A. S. Antigens of Streptococcus mutans: isolation of a serotype-specific and a cross-reactive antigen from walls of strain V-100 (serotype e). Infect Immun. 1978 Jan;19(1):160–169. doi: 10.1128/iai.19.1.160-169.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Wetherell J. R., Jr, Bleiweis A. S. Antigens of Streptococcus mutans: characterization of a polysaccharide antigen from walls of strain GS-5. Infect Immun. 1975 Dec;12(6):1341–1348. doi: 10.1128/iai.12.6.1341-1348.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES