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. 1987 Dec;55(12):3030–3034. doi: 10.1128/iai.55.12.3030-3034.1987

Influence of Staphylococcus aureus antibody on experimental endocarditis in rabbits.

D P Greenberg 1, J I Ward 1, A S Bayer 1
PMCID: PMC260024  PMID: 3679543

Abstract

To evaluate the potential protective benefit of antibody to whole cells of Staphylococcal aureus for the prevention of endocarditis, the rabbit endocarditis model was used. Methicillin-sensitive (17A) and methicillin-resistant (173) S. aureus strains were evaluated in rabbits with or without indwelling intracardiac catheters. All immunized rabbits developed significant homologous agglutinating antibody titers (the mean reciprocal titers were 15,300 to strain 17A and 1,150 to strain 173). After challenge, virtually no significant differences were observed between immunized and unimmunized animals with respect to (i) incidence of endocarditis, (ii) concentration of bacteria in infected vegetations, (iii) incidence of metastatic renal abscesses, or (iv) concentrations of bacteria in infected kidneys. The clearance of homologous S. aureus strains from blood cultures was similar for immunized and unimmunized animals at 10 to 90 min after intravenous challenge. In vivo adherence of homologous S. aureus strains to aortic valves and vegetations was similar in immunized and unimmunized animals when evaluated at 30 and 90 min postchallenge. Even without catheterization, the incidence of bacteremia and renal abscesses was the same in immunized and unimmunized rabbits. Whole-cell-induced S. aureus antibody did not prevent or modify any stage in the development of endocarditis in rabbits.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adler S. W., 2nd, Selinger D. S., Reed W. P. Effect of immunization on the genesis of pneumococcal endocarditis in rabbits. Infect Immun. 1981 Oct;34(1):55–61. doi: 10.1128/iai.34.1.55-61.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Archer G. L., Johnston J. L. Effect of type-specific active immunization on the development and progression of experimental Pseudomonas aeruginosa endocarditis. Infect Immun. 1979 Apr;24(1):167–173. doi: 10.1128/iai.24.1.167-173.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bayer A. S., Lam K. Efficacy of vancomycin plus rifampin in experimental aortic-valve endocarditis due to methicillin-resistant Staphylococcus aureus: in vitro-in vivo correlations. J Infect Dis. 1985 Jan;151(1):157–165. doi: 10.1093/infdis/151.1.157. [DOI] [PubMed] [Google Scholar]
  4. Bayer A. S., Tillman D. B., Concepcion N., Guze L. B. Clinical value of teichoic acid antibody titers in the diagnosis and management of the staphylococcemias. West J Med. 1980 Apr;132(4):294–300. [PMC free article] [PubMed] [Google Scholar]
  5. Cheung A. L., Bayer A. S., Peters J., Ward J. I. Analysis by gel electrophoresis, Western blot, and peptide mapping of protein A heterogeneity in Staphylococcus aureus strains. Infect Immun. 1987 Apr;55(4):843–847. doi: 10.1128/iai.55.4.843-847.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cooper G., Platt R. Staphylococcus aureus bacteremia in diabetic patients. Endocarditis and mortality. Am J Med. 1982 Nov;73(5):658–662. doi: 10.1016/0002-9343(82)90407-7. [DOI] [PubMed] [Google Scholar]
  7. Durack D. T., Gilliland B. C., Petersdorf R. G. Effect of immunization on susceptibility to experimental Streptococcus mutans and Streptococcus sanguis endocarditis. Infect Immun. 1978 Oct;22(1):52–56. doi: 10.1128/iai.22.1.52-56.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Espersen F., Clemmensen I. Isolation of a fibronectin-binding protein from Staphylococcus aureus. Infect Immun. 1982 Aug;37(2):526–531. doi: 10.1128/iai.37.2.526-531.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Espersen F., Frimodt-Møller N. Staphylococcus aureus endocarditis. A review of 119 cases. Arch Intern Med. 1986 Jun;146(6):1118–1121. [PubMed] [Google Scholar]
  10. Espersen F., Schiøtz P. O. Normally-occurring precipitating antibodies against Staphylococcus aureus in human serum and colostrum. Acta Pathol Microbiol Scand C. 1981 Apr;89(2):93–98. doi: 10.1111/j.1699-0463.1981.tb02670.x. [DOI] [PubMed] [Google Scholar]
  11. Finland M., Barnes M. W. Changing etiology of bacterial endocarditis in the antibacterial era. Experiences at Boston City Hospital 1933-1965. Ann Intern Med. 1970 Mar;72(3):341–348. doi: 10.7326/0003-4819-72-3-341. [DOI] [PubMed] [Google Scholar]
  12. Forsgren A. Significance of protein a production by staphylococci. Infect Immun. 1970 Nov;2(5):672–673. doi: 10.1128/iai.2.5.672-673.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Gould K., Ramirez-Ronda C. H., Holmes R. K., Sanford J. P. Adherence of bacteria to heart valves in vitro. J Clin Invest. 1975 Dec;56(6):1364–1370. doi: 10.1172/JCI108216. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Humphreys D. W., Wheat L. J., White A. Staphylococcal heat-stable opsonins. J Lab Clin Med. 1974 Jul;84(1):122–128. [PubMed] [Google Scholar]
  15. Karakawa W. W., Young D. A. Immunological specificity of heat-stable opsonins in immune and nonimmune sera and their interaction with non-encapsulated and encapsulated strains of Staphylococcus aureus. Infect Immun. 1979 Jul;25(1):175–186. doi: 10.1128/iai.25.1.175-186.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Ogawa S. K., Yurberg E. R., Hatcher V. B., Levitt M. A., Lowy F. D. Bacterial adherence to human endothelial cells in vitro. Infect Immun. 1985 Oct;50(1):218–224. doi: 10.1128/iai.50.1.218-224.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Perlman B. B., Freedman L. R. Experimental endocarditis. II. Staphylococcal infection of the aortic valve following placement of a polyethylene catheter in the left side of the heart. Yale J Biol Med. 1971 Oct;44(2):206–213. [PMC free article] [PubMed] [Google Scholar]
  18. Peterson P. K., Wilkinson B. J., Kim Y., Schmeling D., Douglas S. D., Quie P. G., Verhoef J. The key role of peptidoglycan in the opsonization of Staphylococcus aureus. J Clin Invest. 1978 Mar;61(3):597–609. doi: 10.1172/JCI108971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Scheld W. M., Thomas J. H., Sande M. A. Influence of preformed antibody on experimental Streptococcus sanguis endocarditis. Infect Immun. 1979 Sep;25(3):781–785. doi: 10.1128/iai.25.3.781-785.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Scheld W. M., Valone J. A., Sande M. A. Bacterial adherence in the pathogenesis of endocarditis. Interaction of bacterial dextran, platelets, and fibrin. J Clin Invest. 1978 May;61(5):1394–1404. doi: 10.1172/JCI109057. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Shayegani M. Failure of Immune Sera to Enhance Significantly Phagocytosis of Staphylococus aureus: Nonspecific Adsorption of Phagocytosis-Promoting Factors. Infect Immun. 1970 Dec;2(6):742–749. doi: 10.1128/iai.2.6.742-749.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Shayegani M., Hisatsune K., Mudd S. Cell Wall Component Which Affects the Ability of Serum to Promote Phagocytosis and Killing of Staphylococcus aureus. Infect Immun. 1970 Dec;2(6):750–756. doi: 10.1128/iai.2.6.750-756.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Slaughter L., Morris J. E., Starr A. Prosthetic valvular endocarditis. A 12-year review. Circulation. 1973 Jun;47(6):1319–1326. doi: 10.1161/01.cir.47.6.1319. [DOI] [PubMed] [Google Scholar]
  24. Thompson R. L. Staphylococcal infective endocarditis. Mayo Clin Proc. 1982 Feb;57(2):106–114. [PubMed] [Google Scholar]
  25. Toy P. T., Lai L. W., Drake T. A., Sande M. A. Effect of fibronectin on adherence of Staphylococcus aureus to fibrin thrombi in vitro. Infect Immun. 1985 Apr;48(1):83–86. doi: 10.1128/iai.48.1.83-86.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Verbrugh H. A., van Dijk W. C., Peters R., van Erne M. E., Daha M. R., Peterson P. K., Verhoef J. Opsonic recognition of staphylococci mediated by cell wall peptidoglycan: antibody-independent activation of human complement and opsonic activity of peptidoglycan antibodies. J Immunol. 1980 Mar;124(3):1167–1173. [PubMed] [Google Scholar]
  27. Watanakunakorn C., Tan J. S., Phair J. P. Some salient features of Staphylococcus aureus endocarditis. Am J Med. 1973 Apr;54(4):473–481. doi: 10.1016/0002-9343(73)90043-0. [DOI] [PubMed] [Google Scholar]
  28. Weinstein L., Schlesinger J. J. Pathoanatomic, pathophysiologic and clinical correlations in endocarditis (first of two parts). N Engl J Med. 1974 Oct 17;291(16):832–837. doi: 10.1056/NEJM197410172911609. [DOI] [PubMed] [Google Scholar]
  29. Wilson W. R., Jaumin P. M., Danielson G. K., Giuliani E. R., Washington JA I. I., Geraci J. E. Prosthetic valve endocarditis. Ann Intern Med. 1975 Jun;82(6):751–756. doi: 10.7326/0003-4819-82-6-751. [DOI] [PubMed] [Google Scholar]

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